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Streptomyces bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta)

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Abstract

A novel endophytic actinomycete, designated strain NEAU-HZ10T was isolated from moss and characterised using a polyphasic approach. The strain was found to have morphological and chemotaxonomic characteristics typical of the genus Streptomyces. Strain NEAU-HZ10T formed grayish aerial mycelia, which differentiated into straight to flexuous chains of cylindrical spores. The cell wall peptidoglycan was found to contain ll-diaminopimelic acid. Predominant menaquinones were identified as MK-9(H6) and MK-9(H8). The polar lipid profile was found to consist of phosphatidylethanolamine, phosphatidylinositol and two unidentified phospholipids. The major fatty acids were identified as iso-C16:0, anteiso-C15:0 and C16:0. 16S rRNA gene sequence similarity studies showed that strain NEAU-HZ10T belongs to the genus Streptomyces and exhibits high sequence similarity to Streptomyces cocklensis DSM 42063T (98.9 %). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain NEAU-HZ10T clustered with S. cocklensis DSM 42063T, Streptomyces yeochonensis CGMCC 4.1882T (98.7 %), Streptomyces paucisporeus CGMCC 4.2025T (98.4 %) and Streptomyces yanglinensis CGMCC 4.2023T (98.1 %). However, a combination of DNA–DNA hybridisation results and some phenotypic characteristics indicated that strain NEAU-HZ10T can be distinguished from its phylogenetically closely related strains. Therefore, it is proposed that strain NEAU-HZ10T represents a novel species of the genus Streptomyces for which the name Streptomyces bryophytorum sp. nov. is proposed. The type strain is NEAU-HZ10T (= CGMCC 4.7151T = DSM 42138T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Key Technology R&D Program (No. 2012BAD19B06), the National Natural Science Foundation of China (Nos. 31471832, 31171913, 31500010, 31572070 and 31372006) and Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Chuang Li, Pinjiao Jin, Chongxi Liu, Zhaoxu Ma, Junwei Zhao, Jiansong Li, Xiangjing Wang & Wensheng Xiang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Junwei Zhao & Wensheng Xiang

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  1. Chuang Li
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  2. Pinjiao Jin
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  3. Chongxi Liu
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  4. Zhaoxu Ma
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  6. Jiansong Li
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Correspondence to Xiangjing Wang or Wensheng Xiang.

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Li, C., Jin, P., Liu, C. et al. Streptomyces bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta). Antonie van Leeuwenhoek 109, 1209–1215 (2016). https://doi.org/10.1007/s10482-016-0722-5

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