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Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta)

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Abstract

A novel endophytic actinomycete, strain NEAU-TX1-15T, was isolated from moss, collected from Wuchang, Heilongjiang province, north China. A polyphasic taxonomic study was carried out to establish the status of strain NEAU-TX1-15T. Morphological and chemotaxonomic properties of strain NEAU-TX1-15T are consistent with the description of the genus Actinoallomurus. Strain NEAU-TX1-15T was observed to form short spiral or looped spore chains on aerial hyphae. The cell wall peptidoglycan was found to contain lysine and meso-diaminopimelic acid. The major menaquinones were identified as MK-9(H6) and MK-9(H8). The only phospholipid identified was phosphatidylglycerol. The major fatty acid was identified as iso-C16:0. Analysis of the 16S rRNA gene sequence supports the assignment of the novel strain to the genus Actinoallomurus, as it exhibits 99.2 % gene sequence similarity to that of Actinoallomurus yoronensis NBRC 103686T. However, the low level of DNA–DNA relatedness allowed the strain to be differentiated from its close relative. Moreover, strain NEAU-TX1-15T could also be differentiated from A. yoronensis NBRC 103686T and other Actinoallomurus species showing high 16S rRNA gene sequence similarity (>98.0 %) by cultural and physiological characteristics. Therefore, the combination of phenotypic and chemotaxonomic data, and the DNA–DNA hybridization value, indicated that strain NEAU-TX1-15T represents a novel species of the genus Actinoallomurus for which the name Actinoallomurus bryophytorum sp. nov. is proposed. The type strain is NEAU-TX1-15T (=CGMCC 4.7200T = JCM 30340T).

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Acknowledgments

This work was supported in part by grants from the National Outstanding Youth Foundation (No. 31225024), the National Natural Science Foundation of China (No. 31471832, 31171913 and 31372006), the National Key Technology R&D Program (No. 2012BAD19B06), Chang Jiang Scholar Candidates Program for Provincial Universities in Heilongjiang (CSCP), the China Postdoctoral Science Foundation (2014M561319) and the Heilongjiang Postdoctoral Fund (LBH-Z14027).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People’s Republic of China

    Chuang Li, Pinjiao Jin, Weijia Zheng, Liyang Chu, Chongxi Liu, Jiansong Li, Wensheng Xiang & Xiangjing Wang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    Haiyan Wang & Wensheng Xiang

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  1. Chuang Li
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  2. Haiyan Wang
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  3. Pinjiao Jin
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  4. Weijia Zheng
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  5. Liyang Chu
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  8. Wensheng Xiang
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  9. Xiangjing Wang
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Correspondence to Wensheng Xiang or Xiangjing Wang.

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Li, C., Wang, H., Jin, P. et al. Actinoallomurus bryophytorum sp. nov., an endophytic actinomycete isolated from moss (Bryophyta). Antonie van Leeuwenhoek 108, 453–459 (2015). https://doi.org/10.1007/s10482-015-0498-z

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