Abstract
A novel Gram-stain negative, red, rod-shaped, non-motile and aerobic bacterial strain, designated Sy30T, was isolated from dry soils of an abandoned marine saltern at Weihai, China. 16S rRNA sequence analysis indicated that strain Sy30T belongs to the genus Pontibacter in the family Cytophagaceae, with sequence similarities ranging from 93.3 to 96.4 % with other type species of the genus Pontibacter. The predominant cellular fatty acids were iso-C15:0 and summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B). The major menaquinone was MK-7. The major polyamine was sym-homospermidine. The DNA G+C content was 47.7 mol%. The major polar lipids consisted of phosphatidylethanolamine, phosphoaminolipid and two unidentified polar lipid. Based on phenotypic, chemotaxonomic and phylogenetic analysis, strain Sy30T represents a novel species of the genus Pontibacter in the family Cytophagaceae, phylum Bacteroidetes, for which the name Pontibacter locisalis sp. nov. is proposed. The type strain is Sy30T (=KCTC 42498T = CICC AB 2015060T).
Similar content being viewed by others
References
Akagawa M, Yamasato K (1989) Synonymy of Alcafigenes aquamarinus, Alcaligenes faecalis subsp. homari, and Deleya aesta: Deleya aquamarina comb. nov. as the type species of the genus Deleya. Int J Syst Bacteriol 3:462–466
Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
Busse HJ, Auling G (1988) Polyamine patterns as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11:1–8
Cao HJ, NieY Zeng XC, Xu LH, He ZC, Luo XS, Wu RN (2014) Pontibacter yuliensis sp nov., isolated from soil. Int J Syst Evol Microbiol 64:968–972
Cowan ST, Steel KJ (1965) Manual for the Identification of Medical Bacteria, vol 11. Cambridge University Press, London, pp 1–8
Dai J, Xu MB, Peng F, Jiang F, Chen X, Wang Z, Fang CX (2014) Pontibacter soli sp nov., isolated from the soil of a Populus rhizosphere in Xinjiang, China. Antonie Van Leeuwenhoek 105:65–72
Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ (2014) Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64:1690–1696
Dwivedi V, Niharika N, Lal R (2013) Pontibacter lucknowensis sp nov., isolated from a hexachlorocyclohexane dump site. Int J Syst Evol Microbiol 63:309–313
Fautz E, Reichenbach H (1980) A simple test for flexirubin-type pigments. FEMS Microbiol Ecol 8:87–91
Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by highperformance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469
Joung Y, Kim H, Ahn TS, Joh K (2011) Pontibacter salisaro sp nov., Isolated from a clay tablet solar saltern in Korea. J Microbiol 49:290–293
Joung Y, Kim H, Lee BI, Kang H, Jang TY, Kwon OS, Joh K (2013) Pontibacter jeungdoensis sp nov., Isolated from a Solar Saltern in Korea. J Microbiol 51:531–535
Kang JY, Joung Y, Chun J, Kim H, Joh K, Jahng KY (2013) Pontibacter saemangeumensis sp nov., isolated from seawater. Int J Syst Evol Microbiol 63:565–569
Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721
Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64:2204–2209
Mahato NK, Tripathi C, Nayyar N, Singh AK, Lal R (2015) Pontibacter ummariensis sp. nov., isolated from a hexachlorocyclohexane contaminated soil. Int J Syst Evol Microbiol. doi:10.1099/ijsem.0.000840
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Nedashkovskaya OI, Kim SB, Suzuki M, Shevchenko LS, Lee, Lee KH, Park MS, Frolova GM, Oh HW, Bae KS, Park HY, Mikhailov VV (2005) Pontibacter actiniarum gen. nov., sp nov., a novel member of the phylum ‘Bacteroidetes’, and proposal of Reichenbachiella gen. nov as a replacement for the illegitimate prokaryotic generic name Reichenbachia Nedashkovskaya et al. 2003. Int J Syst Evol Microbiol 55:2583–2588
Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. MIDI Inc, Newark
Schlesner H, Bartels C, Sittig M, Dorsch M, Stackebrandt E (1990) Taxonomic and phylogenetic studies on a new taxon of budding, hyphal Proteobacteria, Hirschia baltica gen.nov., sp. nov. Int J Syst Bacteriol 40:443–451
Singh AK, Garg N, Sangwan N, Negi V, Kumar R, Vikram S, Lal R (2013) Pontibacter ramchanderi sp nov., isolated from hexachlorocyclohexane-contaminated pond sediment. Int J Syst Evol Microbiol 63:2829–2834
Singh AK, Garg N, Lata P, Kumar R, Negi V, Vikram S, Lal R (2014) Pontibacter indicus sp nov., isolated from hexachlorocyclohexane-contaminated soil. Int J Syst Evol Microbiol 64:254–259
Singh AK, Garg N, Lal R (2015) Pontibacter chinhatensis sp. nov., isolated from pond sediment containing discarded hexachlorocyclohexane isomer waste. Int J Syst Evol Microbiol 65:2248–2254
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhard P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, pp 607–654
Srinivasan S, Lee JJ, Lee SS, Kim M (2014) Pontibacter humi sp nov., isolated from mountain soil. Curr Microbiol 69:263–269
Subhash Y, Tushar L, Sasikala C, Ramana CV (2013) Erythrobacter odishensis sp nov and Pontibacter odishensis sp nov isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63:4524–4532
Subhash Y, Sasikala C, Ramana CV (2014) Pontibacter ruber sp nov and Pontibacter deserti sp nov., isolated from the desert. Int J Syst Evol Microbiol 64:1006–1011
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Wang Y, Zhang KD, Cai F, Zhang L, Tang YL, Dai J, Fang CX (2010) Pontibacter xinjiangensis sp nov., in the phylum ‘Bacteroidetes’, and reclassification of [Effluviibacter] roseus as Pontibacter roseus comb. nov. Int J Syst Evol Microbiol 60:99–103
Xu M, Xin Y, Yu Y, Zhang J, Zhou Y, Liu H, Tian J, Li Y (2010) Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60:2215–2220
Xu MB, Wang Y, Dai J, Jiang F, Rahman E, Peng F, Fang CX (2012) Pontibacter populi sp nov., isolated from the soil of a Euphrates poplar (Populus euphratica) forest. Int J Syst Evol Microbiol 62:665–670
Xu LH, Zeng XC, Nie Y, Luo XS, Zhou EM, Zhou LL, Pan YF, Li WJ (2014) Pontibacter diazotrophicus sp nov., a novel nitrogen-fixing bacterium of the family Cytophagaceae. PLoS One 9:1–9
Zhang L, Zhang QJ, Luo XS, Tang Y, Dai J, Li YW, Wang Y, Chen G, Fang CX (2008) Pontibacter korlensis sp nov., isolated from the desert of Xinjiang, China. Int J Syst Evol Microbiol 58:1210–1214
Zhang L, Zhu LF, Wei LF, Li CF, Wang Y, Shen XH (2013) Pontibacter toksunensis sp nov., isolated from soil, and emended descriptions of Pontibacter roseus and Pontibacter akesuensis. Int J Syst Evol Microbiol 63:4462–4468
Acknowledgments
This work was supported by the National Natural Science Foundation of China (31370057, 31370108), National Science and Technology Major Project of China (2013ZX10004217) and 2013 Shandong Provincial Second Group Projects on Resource Platforms for Marine Economic and Innovative Development Regions: Marine Microorganisms Preservation Platform (2150299). We thank China Center for type Culture Collection (CCTCC) for kindly providing the type strains, Pontibacter xinjiangensis CCTCC AB 207200T and Pontibacter korlensis CCTCC AB 206081T.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhou, YX., Xie, ZH., Zhao, JX. et al. Pontibacter locisalis Sy30T sp. nov. isolated from soil collected from an abandoned saltern. Antonie van Leeuwenhoek 109, 415–420 (2016). https://doi.org/10.1007/s10482-016-0646-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10482-016-0646-0