Abstract
A gram-stain-negative, endo-spore forming, facultatively anaerobic, motile, rod-shaped bacterial strain SM69T, isolated from soil samples of Rohtak, Haryana, India was characterized using polyphasic approach. White colonies were 2–3 mm, in diameter and growth occurred between 20 and 55 °C, pH 6.0–10.0 with 0–2.0% (w/v) NaCl. Based on 16S rRNA gene sequence similarity the strain is placed in the genus Paenibacillus as it is closely related to ‘Paenibacillus tyrfis MSt1T’ (99.7%) and P. elgii SD17T (99.6%). The cell wall peptidoglycan contained meso-diaminopimelic acid. The dominant fatty acids included anteiso-C15: 0 (50%), C16: 0 (12%) and anteiso-C17: 0 (10%). Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The size of the draft genome was 7,848,017 bp, with 53.1% G+C content. dDDH (51.6%) and ANI (93.5%) of strain SM69T with its close relatives indicates that it represents a novel species, for which the name Paenibacillus oleatilyticus sp. nov. (Type strain SM69T = MCC 3064T = JCM 33981T = KACC 21649T) is proposed.
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Data availability
The GenBank/EMBL/DDBJ accession numbers for the reference 16S rRNA gene sequence and whole genome sequences of strain SM69T are MN174065 and JAHNZO000000000, respectively.
Abbreviations
- P.:
-
Paenibacillus
- FAME:
-
Fatty acid methyl esters
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization.
- DPG:
-
Diphosphatidylglycerol
- PE:
-
Phosphatidylethanolamine
- PG:
-
Phosphatidylglycerol
- PL:
-
Unknown phospholipid
- APL:
-
Unknown amino phospholipid
- L:
-
Unknown lipid
- API:
-
Analytical profile index
- %T:
-
Transmittance
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Acknowledgements
We thank Professor Bernhard Schink for his valuable help in nomenclature of the species. We appreciated the help from Ms. Mily Prakash Modi for phenotypic and chemotaxonomic characterization. We thank Ms. Prachi Karodi for polar lipid analysis and Mrs. Sonia Thite for BIOLOG analysis. Special thanks to Dr. Tapan Chakrabarti for his valuable suggestions for writing this manuscript.
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Authors acknowledge the funding by Department of Biotechnology (DBT), Govt. of India under the grant number BT/Coord.II/01/03/2016 (NCMR) used for in-house laboratory facilities.
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NSC did the isolation of strain SM69T and conceptualized; SS carried out the polyphasic taxonomy experiments; SS, AAJ and TL did the phenotypic and chemotaxonomic analysis; NC, DD and TL did the genome data analysis and wrote the genome part of the manuscript; YS provided the project funding and in house facilities. NJ conceived of or designed study, led the investigation, did the formal analysis and wrote the first draft of the manuscript. AAJ, TL and NJ reviewed, edited and finalized the manuscript. All the authors reviewed and approved the final version of the paper.
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Chauhan, N.S., Joseph, N., Shaligram, S. et al. Paenibacillus oleatilyticus sp. nov., isolated from soil. Arch Microbiol 204, 516 (2022). https://doi.org/10.1007/s00203-022-03116-0
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DOI: https://doi.org/10.1007/s00203-022-03116-0