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Novosphingobium tardum sp. nov., isolated from sediment of a freshwater lake

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A Gram-negative, non-motive, aerobic and non-spore-forming strain 16-28-2T isolated from freshwater sediment of Taihu Lake was characterized by using a polyphasic approach. The optimum growth conditions were found to be as follows: 28 °C, pH 6.5 and 0–0.5 % NaCl in YG liquid medium. The major fatty acids were identified to be summed feature 3 (consisting of C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (consisting of C18:1 ω7c and/or C18:1 ω6c), C14:0 2-OH, C17:1 ω6c, C16:0 and C18:1 ω7c 11-methyl (>5 %). Strain 16-28-2T was found to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid as the major polar lipids; and ubiquinone 10 (Q-10) as the major respiratory quinone. DNA G+C content of strain 16-28-2T was 63.5 mol % (Tm). A phylogenetic study of 16S rRNA gene indicated that strain 16-28-2T is a member of the genus Novosphingobium, with the highest 16S rRNA gene sequence similarity of 96.3 % with Novosphingobium lentum MT1T and below 96 % with the other Novosphingobium species. On the basis of the phylogenetic, phenotypic analyses and biochemical characterization, we suggest that strain 16-28-2T is a novel species in the genus Novosphingobium, for which the name Novosphingobium tardum sp. nov. is proposed. The type strain of N. tardum is 16-28-2T (=CGMCC 1.12989T =NBRC 110956T).

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Acknowledgments

This work was supported by the project No.31070105 of the National Natural Science Foundation of China. It was also supported by China Geological Survey Grant (No. 12120113015200).

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Authors and Affiliations

  1. State Key Laboratory of Agrobiotechnology, Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, Center of Life Science, China Agricultural University, Room 1061, No. 2 Yuanmingyuanxilu, Haidian District, Beijing, 100193, China

    Nan Chen, Xue-Jian Yu, Jin-Shui Yang, Bao-Zhen Li & Hong-Li Yuan

  2. Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, 11340, México, DF, Mexico

    En-Tao Wang

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  1. Nan Chen
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  2. Xue-Jian Yu
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  3. Jin-Shui Yang
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  5. Bao-Zhen Li
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Correspondence to Hong-Li Yuan.

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Chen, N., Yu, XJ., Yang, JS. et al. Novosphingobium tardum sp. nov., isolated from sediment of a freshwater lake. Antonie van Leeuwenhoek 108, 51–57 (2015). https://doi.org/10.1007/s10482-015-0463-x

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  • DOI: https://doi.org/10.1007/s10482-015-0463-x

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