Abstract
A novel Gram-stain negative, yellow coloured, strictly aerobic, rod-shaped, non-motile bacterium designated as THW-SA1T, was isolated from lake water near Samsung apartment, Suwon, Republic of Korea. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain THW-SA1T belongs to the genus Novosphingobium and is closely related to Novosphingobium taihuense (97.8 %) and Novosphingobium subterraneum (97.1 %). The DNA–DNA relatedness values between strain THW-SA1T and the most closely related type strains were found to be less than 30.0 %. The DNA G+C content was determined to be 67.5 mol%. The strain grows optimally at 25–28 °C, at pH 7.0, and in the presence of 0.5 % NaCl. The predominant isoprenoid quinone was identified as ubiquinone Q-10. The polar lipid profile comprises diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sphingoglycolipid, phosphatidylcholine, some unidentified phospholipids and some unidentified polar lipids. Fatty acids characteristic for this genus, such as C16:1, C14:0 2-OH, C16:1 ω6c and/or C16:1 ω7c (summed feature 3) and C18:1 ω6c and/or C18:1 ω7c (summed feature 8) were also detected. On the basis of the phenotypic and genotypic analysis, the strain THW-SA1T is considered to represent a novel species of the genus Novosphingobium, for which the name Novosphingobium aquaticum sp. nov. is proposed. The type strain is THW-SA1T (=KCTC 42608T=CCTCC AB 2015114T).
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Acknowledgments
This work was conducted under the industrial infrastructure program (No. N0000888) for fundamental technologies which is funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).
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The NCBI GenBank accession number for the 16S rRNA gene sequence of strains THW-SA1T is KP410550.
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Fig. S1
Transmission electron micrographs of strain THW-SA1T. After negative staining with uranyl acetate. Bar indicated 200 nm (PDF 59 kb)
Fig. S2
The maximum likelihood tree based on 16S rRNA gene sequence analysis showing phylogenetic relationships between strain THW-SA1T and members of the genus Novosphingobium. Bootstrap values less than 60 % were not indicated. Sphingomonas jaspsi TDMA-16T was used as an out group. Scale bar, 0.02 substitutions per nucleotide position (PDF 191 kb)
Fig. S3
Two-dimensional TLC of the total polar lipids of strain THW-SA1T (a) and Novosphingobium taihuense KACC 18096T (b), stained for total polar lipids with 5 % ethanolic molybdatophosphoric acid. Abbreviations: DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PDE, phosphatidyldimethylethanolamine, SGL sphingoglycolipid, PC, phosphatidylcholine; PL1-3, unidentified phospholipids; L1-4, unidentified polarlipids (PDF 16 kb)
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Singh, H., Du, J., Yang, JE. et al. Novosphingobium aquaticum sp. nov., isolated from lake water in Suwon, Republic of Korea. Antonie van Leeuwenhoek 108, 851–858 (2015). https://doi.org/10.1007/s10482-015-0539-7
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DOI: https://doi.org/10.1007/s10482-015-0539-7