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Draconibacterium filum sp. nov., a new species of the genus of Draconibacterium from sediment of the east coast of the Korean Peninsula

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Abstract

A Gram-stain negative, long rod shaped, facultatively anaerobic bacterium, designated strain F2T, was isolated from coastal sediment of the Korean Peninsula. Strain F2T was found to grow at 10–40 °C (optimum 30 °C), at pH 6.0–8.5 (optimum pH 7.5) and at 0.0–8.0 % (w/v) NaCl (optimum 3.0 %). Phylogenetic analysis of the 16S rRNA gene sequence showed that strain F2T is closely related to Draconibacterium orientale FH5T (with 97.9 % 16S rRNA gene similarity) of the family Prolixibacteraceae of the phylum Bacteroidetes. The major isoprenoid quinone was identified as MK-7 and the main fatty acids as iso-C15:0 (24.1 %), anteiso-C15:0 (15.4 %), C16:0 (10.7 %), iso-C17:0 3-OH (7.6 %) and iso-C16:0 3-OH (5.9 %). The major polar lipids were identified as phosphatidylethanolamine, phosphatidylinositol and four unidentified polar lipids. The genomic DNA G+C content of strain F2T was determined to be 44.7 mol% and the DNA–DNA relatedness of strain F2T with D. orientale DSM 25947T was 34.6 ± 4.3 %. Nitrate reduction capability and cell morphology of strain F2T are distinct from those of the closest relative, D. orientale DSM 25947T. Based on these properties, we propose strain F2T represents a novel species within the genus Draconibacterium, with the name Draconibacterium filum sp. nov. The type strain of D. filum is F2T (=KCTC 32486T = JCM 19986T).

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Acknowledgments

This work was supported by the Korea Polar Research Institute (PP12010) of the Ministry of Land, Transportation and Maritime Affairs, Republic of Korea, the Basic Science Research Program (2012R1A1A2A10039384), and the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted from the Ministry of Trade, Industry & Energy, Republic of Korea (20132020000170).

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Authors and Affiliations

  1. Department of Microbiology, Chungbuk National University, Cheongju, 362-763, Republic of Korea

    Joo-Han Gwak, So-Jeong Kim, Man-Young Jung, Jong-Geol Kim & Sung-Keun Rhee

  2. Korea Basic Science Institute, Daejeon, 305-806, Republic of Korea

    Seong Woon Roh & Kyung June Yim

  3. Department of Biology, Jeju National University, Jeju, 690-756, Republic of Korea

    Kyung June Yim & Soo-Je Park

  4. Microbial Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Yong-Jae Lee & Song-Gun Kim

  5. University of Science and Technology, Yuseong-gu, Daejeon, 305-850, Republic of Korea

    Song-Gun Kim

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  1. Joo-Han Gwak
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Correspondence to Sung-Keun Rhee.

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10482_2015_396_MOESM1_ESM.docx

Fig. S1. Two-dimensional TLC showing the polar lipids profile of strain F2T stained with molybdatophosphoric acid (Merck). Solvents used were as follows: first direction, chloroform/methanol/water (65:25:4, v/v/v); second direction, chloroform/methanol/acetic acid/water (80:12:15:4, v/v/v/v). PE, phosphatidylethanolamine; PI, phosphatidylinositol; L1-4, unidentified polar lipids (DOCX 2413 kb)

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Gwak, JH., Kim, SJ., Jung, MY. et al. Draconibacterium filum sp. nov., a new species of the genus of Draconibacterium from sediment of the east coast of the Korean Peninsula. Antonie van Leeuwenhoek 107, 1049–1056 (2015). https://doi.org/10.1007/s10482-015-0396-4

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