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Draconibacterium halophilum sp. nov., A Halophilic Bacterium Isolated from Marine Sediment

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Abstract

A Gram-stain-negative, long-rod shaped, and facultatively anaerobic bacterium, designated as strain M1T, was isolated from the marine sediment of Jeju Island, South Korea. Strain M1T was found to be catalase- and oxidase-positive, light yellow-pigmented, non-motile, and non-flagellated, growing optimally at 30 °C, pH 7.0, and in the presence of 3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain M1T belongs to the genus Draconibacterium and is closely related to Draconibacterium orientale FH5T (97.2%), Draconibacterium sediminis JN14CK-3 T (96.5%), “Draconibacterium filum” F2T (96.5%) and Draconibacterium mangrovi GM2-18 T (96.3% sequence similarity). The values for digital DNA-DNA hybridization ranged from 37.6 to 38.3% against D. orientale FH5T, D. sediminis KN14CK-3 T, and D. mangrovi GM2-18 T, clearly indicating that strain M1T represents a distinct species of the genus Draconibacterium. Strain M1T has a 40.0% G + C content estimated by genome sequence, menaquinone 7 as the sole respiratory quinone, C15:0 anteiso and C15:0 iso as the major fatty acids, and phosphatidylethanolamine, an unidentified phospholipid, and unidentified lipids as the polar lipids. Based on the polyphasic characteristics, it is suggested that strain M1T be assigned to the genus Draconibacterium as the type strain of a novel species, for which the name Draconibacterium halophilum sp. nov. is proposed. The type strain is M1T (= KCTC 72809 T = VTCC 910107 T).

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Abbreviations

ANI:

Average nucleotide identity

MA:

Marine agar

MB:

Marine broth

dDDH:

Digital DNA-DNA hybridization

AAI:

Average amino acid identity

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Acknowledgements

This work was supported by grants from the National Research Foundation of Korea (No. 2020R1I1A3062110) and National Institute of Biological Resources funded by the Ministry of Environment (Nos. NIBR202102109 and NIBR202104104).

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Authors and Affiliations

  1. Department of Biology, Jeju National University, 102 Jejudaehak-ro, Jeju, 63243, South Korea

    Minji Kim & Soo-Je Park

  2. Microorganism Resources Division, National Institute of Biological Resources, Incheon, 22689, South Korea

    Ki-Eun Lee & In-Tae Cha

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  1. Minji Kim
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  2. Ki-Eun Lee
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Contributions

MK and SJP designed the experiments. MK, KEL, and SJP performed the experiments. MK, ITC, and SJP analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Soo-Je Park.

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The GenBank/EMBL/DDBJ accession numbers for the whole-genome sequence and 16S rRNA gene sequence of strain M1T are CP048409 and MK828353, respectively.

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Kim, M., Lee, KE., Cha, IT. et al. Draconibacterium halophilum sp. nov., A Halophilic Bacterium Isolated from Marine Sediment. Curr Microbiol 78, 2440–2446 (2021). https://doi.org/10.1007/s00284-021-02496-8

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  • DOI: https://doi.org/10.1007/s00284-021-02496-8

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