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Idiomarina atlantica sp. nov., a marine bacterium isolated from the deep sea sediment of the North Atlantic Ocean

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Abstract

An aerobic Gram-negative, slightly curved rod-shaped and non-motile bacterium, designated G5_TVMV8_7T, was isolated from the deep sea sediment sample of the North Atlantic Ocean. Strain G5_TVMV8_7T displayed growth at 4–45 °C (optimum, 25–35 °C), at pH 6.0–11.0 (optimum, 7.0–8.0) and in 1.0–15.0 % (w/v) NaCl (optimum, 3.0–5.0 %). Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain G5_TVMV8_7T belongs to the genus Idiomarina, sharing the highest similarity with Idiomarina salinarum ISL-52T (97.4 %) and I. homiensis PO-M2T (97.1 %) followed by other validly described species of the genus Idiomarina (96.9–93.5 %). The G + C content of the genomic DNA was found to be 50.2 mol %. The digital DNA–DNA hybridization value between strain G5_TVMV8_7T and I. salinarum ISL-52T was 17.70 ± 2.24 %. The average nucleotide identity values between strain G5_TVMV8_7T and I. salinarum ISL-52T was found to be 70.03 %. The major fatty acids were C16:0 (12.7 %), iso-C15:0 (11.5 %) and Sum In Feature 8 (C18:1 ω7c/ω6c) (13.5 %). The predominant respiratory quinone was identified as Q-8, and the polar lipids as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol including several unidentified polar lipids. Based on the genotypic and phenotypic results, a new species Idiomarina atlantica is proposed. The type strain is G5_TVMV8_7T (= MCCC 1A10513T = KCTC 42141T).

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Abbreviations

MCCC:

Marine microorganisms culture collection of China

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Acknowledgments

This work was financially supported by National Infrastructure of Microbial Resources of China (No. NIMR-2013-9 and NIMR-2014-9) and COMRA program (No. DY125-15-R-01).

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Authors and Affiliations

  1. State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, The Third Institute of State Oceanic Administration; Key Laboratory of Marine Genetic Resources of Fujian Province; Collaborative Innovation Center of Deep Sea Biology; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361005, China

    Juan Du, Qiliang Lai, Yang Liu, Yaping Du, Xiupian Liu, Fengqin Sun & Zongze Shao

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  1. Juan Du
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  7. Zongze Shao
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Correspondence to Zongze Shao.

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Du, J., Lai, Q., Liu, Y. et al. Idiomarina atlantica sp. nov., a marine bacterium isolated from the deep sea sediment of the North Atlantic Ocean. Antonie van Leeuwenhoek 107, 393–401 (2015). https://doi.org/10.1007/s10482-014-0337-7

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