Skip to main content
SpringerLink
Log in

Fictibacillus enclensis sp. nov., isolated from marine sediment

  • Original Paper
  • Published:
Antonie van Leeuwenhoek Aims and scope Submit manuscript

Abstract

A novel Gram-positive strain, designated NIO-1003T, was isolated from a marine sediment sample collected from the Chorao Island, Goa Provence, India. Strain NIO-1003T was found to be strictly aerobic, motile, endospore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1003T belongs to the genus Fictibacillus and to be most closely related to Fictibacillus rigui KCTC 13278T, Fictibacillus solisalsi KCTC 13181T and Fictibacillus barbaricus DSM 14730T with 98.2, 98.0 and 97.2 % similarity and 25, 28, 39 nucleotide differences respectively. Strain NIO-1003T was characterized by having cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor amounts of an aminophospholipid were detected. The major fatty acids were identified as ai-C15:0, iso-C15:0, ai-C17:0 and C16:0, supporting the grouping of strain NIO-1003T into the family Bacillaceae. The DNA G+C content of strain NIO-1003T was determined to be 42.6 mol%. On the basis of phenotypic properties, phylogeny and DNA–DNA hybridisation analysis, strain NIO-1003T is considered to represent a novel species of the genus Fictibacillus for which the name Fictibacillus enclensis sp. nov. is proposed. The type strain is NIO-1003T (= NCIM 5458T = DSM 25142T).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Ahmed I, Yokota A, Yamazoe A, Fujiwara T (2007) Proposal of Lysinibacillus boronitolerans gen. nov., sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov. Int J Syst Evol Microbiol 57:1117–1125

    Article  CAS  PubMed  Google Scholar 

  • Albert RA, Archambault J, Lempa M, Hurst B, Richardson C, Gruenloh S, Duran M, Worliczek HL, Huber BE et al (2007) Proposal of Viridibacillus gen. nov. and reclassification of Bacillus arvi, Bacillus arenosi and Bacillus neidei as Viridibacillus arvi gen. nov., comb. nov., Viridibacillus arenosi comb. nov. and Viridibacillus neidei comb. nov. Int J Syst Evol Microbiol 57:2729–2737

    Article  CAS  PubMed  Google Scholar 

  • Ash C, Priest FG, Collins MD (1993) Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. Antonie Van Leeuwenhoek 64:253–260

    Article  CAS  PubMed  Google Scholar 

  • Baik KS, Lim CH, Park SC, Kim EM, Rhee MS, Seong CN (2010) Bacillus rigui sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 60:2204–2209

    Article  CAS  PubMed  Google Scholar 

  • Claus D, Berkeley RCW (1986). Genus Bacillus Cohn 1872. In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 1105–1140. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specified tree topology. Syst Zool 20(4):406–416

    Article  Google Scholar 

  • Fortina MG, Pukall R, Schumann P, Mora D, Parini C, Manachini PL, Stackebrandt E et al (2001) Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson,1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov. Int J Syst Evol Microbiol 51:447–455

    Article  CAS  PubMed  Google Scholar 

  • Gillis M, De Ley J, De Cleene M (1970) The determination of molecular weight of bacterial genome DNA from renaturation rates. Eur J Biochem 12:143–153

    Google Scholar 

  • Glaeser SP, Wolfgang D, Busse H-J, Kämpfer P (2013) Fictibacillus phosphorivorans gen. nov., sp. nov. and proposal to reclassify Bacillus arsenicus, Bacillus barbaricus, Bacillus macauensis, Bacillus nanhaiensis, Bacillus rigui, Bacillus solisalsi and Bacillus gelatini in the genus Fictibacillus. Int J Syst Evol Microbiol 63:2934–2944

    Article  CAS  PubMed  Google Scholar 

  • Hatayama K, Shoun H, Ueda Y, Nakamura A (2006) Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura, 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov. Int J Syst Evol Microbiol 56:2545–2551

  • Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65:501–509

    Article  CAS  Google Scholar 

  • Heyndrickx M, Lebbe L, Kersters K, De Vos P, Forsyth G, Logan NA (1998) Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48:99–106

    Article  Google Scholar 

  • Huss VAR, Festl H, Schleifer KH (1983) Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192

    Article  CAS  PubMed  Google Scholar 

  • Jeon CO, Lim JM, Lee JM, Xu LH, Jiang CL, Kim CJ (2005) Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55:1891–1896

    Article  CAS  PubMed  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16(2):111–120

    Article  CAS  PubMed  Google Scholar 

  • Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver-loaded ion exchanger. J Liq Chromatogr 5:2359–2367

    Article  CAS  Google Scholar 

  • Leifson E (1960) Atlas of Bacterial Flagellation. Academic Press, London

    Google Scholar 

  • Ley De, Cattoir JH, Reynaerts A (1970) The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142

    Article  PubMed  Google Scholar 

  • Li W-J, Xu P, Schumann P, Zhang Y-Q, Pukall R, Xu L-H, Stackebrandt E, Jiang C-L (2007) Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57:1424–1428

    Article  PubMed  Google Scholar 

  • Liu H, Zhou Y, Liu R, Zhang K-Y, Lai R (2009) Bacillus solisalsi sp. nov., a halotolerant, halophilic bacterium isolated from soil around a salt lake. Int J Syst Evol Microbiol 59:1460–1464

    Article  CAS  PubMed  Google Scholar 

  • Loveland-Curtze J, Vanya IM, Jean EB (2011) Evaluation of a new fluorimetric DNA–DNA hybridization method. Can J Microbiol 57:250–255

    Article  CAS  PubMed  Google Scholar 

  • Marmur J (1961) A procedure for isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218

    Article  CAS  Google Scholar 

  • Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118

    Article  CAS  PubMed  Google Scholar 

  • Meier-Kolthoff JP, Göker M, Spröer C, Klenk HP (2013) When should a DDH experiment be mandatory in microbial taxonomy? Arch Microbiol 195:413–418

    Article  CAS  PubMed  Google Scholar 

  • Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal K, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  • Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA, Ivanova AE, Lysenko AM, Petrunyaka VV, Osipov GA et al (2001) Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int J Syst Evol Microbiol 51:433–446

    CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20:1–6

    Google Scholar 

  • Shida O, Takagi H, Kadowaki K, Komagata K (1996) Proposal for two new genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46:939–946

    Article  CAS  PubMed  Google Scholar 

  • Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155

    Google Scholar 

  • Staneck JL, Roberts GD (1974) Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28:226–231

    CAS  PubMed Central  PubMed  Google Scholar 

  • Tamaoka J, Komagata K (1984) Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  CAS  Google Scholar 

  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol 28(10):2731–2739

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Täubel M, Kämpfer P, Buczolits S, Lubitz W, Busse H-J (2003) Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int J Syst Evol Microbiol 53:725–730

    Article  PubMed  Google Scholar 

  • Tcherpakov M, Ben-Jacob E, Gutnick DL (1999) Paenibacillus dendritiformis sp. nov., proposal for a new pattern-forming species and its localization within a phylogenetic cluster. Int J Syst Bacteriol 49:239–246

    Article  CAS  PubMed  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Vaishampayan P, Miyashita M, Ohnishi A, Satomi M, Rooney A, La Duc MT, Venkateswaran K et al (2009) Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura, 2002 as Rummeliibacillus pycnus comb. nov. Int J Syst Evol Microbiol 59:1094–1099

    Article  CAS  PubMed  Google Scholar 

  • Wainø M, Tindall BJ, Schumann P, Ingvorsen K (1999) Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov. Int J Syst Bacteriol 49:821–831

    Article  PubMed  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Google Scholar 

  • Wisotzkey JD, Jurtshuk P Jr, Fox GE, Deinhard G, Poralla K (1992) Comparative sequence analysis on the 16S rRNA (rDNA) of Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus and proposal for creation of a new genus, Alicyclobacillus gen. nov. Int J Syst Bacteriol 42:263–269

    Article  CAS  PubMed  Google Scholar 

  • Yoon J-H, Yim DK, Lee J-S, Shin K-S, Sato HH, Lee ST, Park YK, Park Y-H (1998) Paenibacillus campinasensis sp. nov., a cyclodextrin-producing bacterium isolated in Brazil. Int J Syst Bacteriol 48:833–837

    Article  CAS  PubMed  Google Scholar 

  • Yoon J-H, Weiss N, Lee K-C, Lee I-S, Kang KH, Park Y-H (2001a) Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, and reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Evol Microbiol 51:2087–2093

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Lee KC, Weiss N, Kho YH, Kang KH, Park YH (2001b) Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov. and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina. Int J Syst Evol Microbiol 51:1079–1086

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang SJ, Oh TK et al (2007) Reclassification of Marinococcus albus Hao, 1985 as Salimicrobium album gen. nov., comb. nov. and Bacillus halophilus Ventosa et al. 1990 as Salimicrobium halophilum comb. nov., and description of Salimicrobium luteum sp. nov. Int J Syst Evol Microbiol 57:2406–2411

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

SGD acknowledges the financial supports received under the Start up Grant Nos. MLP-027426 from the CSIR- National Chemical Laboratory, Pune, India.

Author information

Authors and Affiliations

  1. NCIM-Resource Center, CSIR-National Chemical Laboratory, Pune, 411008, Maharashtra, India

    Syed G. Dastager & Rahul Mawlankar

  2. Microbial Type Culture Collection and Gene Bank (MTCC), CSIR-Institute of Microbial Technology, Sector-39A, Chandigarh, 160036, India

    Krishnamurthi Srinivasan

  3. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education and Laboratory for Conservation and Utilization of Bio-resources, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Shan-Kun Tang

  4. Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Taejon, 305-806, Republic of Korea

    Jae-Chan Lee

  5. Microbial Culture Collection (MCC), National Centre for Cell Science, Pune, 411007, Maharashtra, India

    V. Venkata Ramana & Yogesh S. Shouche

Authors
  1. Syed G. Dastager
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rahul Mawlankar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Krishnamurthi Srinivasan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shan-Kun Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jae-Chan Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. Venkata Ramana
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yogesh S. Shouche
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Syed G. Dastager.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 414 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dastager, S.G., Mawlankar, R., Srinivasan, K. et al. Fictibacillus enclensis sp. nov., isolated from marine sediment. Antonie van Leeuwenhoek 105, 461–469 (2014). https://doi.org/10.1007/s10482-013-0097-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10482-013-0097-9

Keywords

Search

Navigation