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Lactobacillus ginsenosidimutans sp. nov., isolated from kimchi with the ability to transform ginsenosides

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Abstract

Biotransformation of ginsenosides was examined using lactic acid bacteria isolated from several kinds of kimchi. A Gram-positive, facultatively anaerobic, non-motile, non-spore-forming, and rod-shaped lactic acid bacterial strain, designated EMML 3041T, was determined to have ginsenoside-converting activity and its taxonomic position was investigated using a polyphasic approach. Strain EMML 3041T displayed β-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to F2 via gypenoside XVII, ginsenoside Rb2 to compound Y via compound O, ginsenoside Rc to compound Mc via compound Mc1, and ginsenoside Rd to ginsenoside F2. On the basis of the 16S rRNA gene sequence similarity, strain EMML 3041T was shown to belong to the genus Lactobacillus and is closely related to Lactobacillus versmoldensis KU-3T (98.3 % sequence similarity). Polyphasic taxonomy study confirmed that the strain EMML 3041T represents a novel species, for which the name Lactobacillus ginsenosidimutans sp. nov. is proposed, with EMML 3041T (=KACC 14527T = JCM 16719T) as the type strain.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 program (No. PJ008193) of Rural Development Administration and by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2011-0031967), and by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment, Republic of Korea.

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Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Hae-Min Jung, Sung-Taik Lee & Sun-Chang Kim

  2. KI for Biocentury, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon, 305-701, Republic of Korea

    Qing-Mei Liu, Jin-Kwang Kim, Sun-Chang Kim & Wan-Taek Im

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  1. Hae-Min Jung
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  2. Qing-Mei Liu
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  3. Jin-Kwang Kim
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  4. Sung-Taik Lee
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  5. Sun-Chang Kim
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  6. Wan-Taek Im
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Correspondence to Wan-Taek Im.

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Hae-Min Jung and Qing-Mei Liu contributed equally to this study.

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Jung, HM., Liu, QM., Kim, JK. et al. Lactobacillus ginsenosidimutans sp. nov., isolated from kimchi with the ability to transform ginsenosides. Antonie van Leeuwenhoek 103, 867–876 (2013). https://doi.org/10.1007/s10482-012-9868-y

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