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Paenibacillus brassicae sp. nov., isolated from cabbage rhizosphere in Beijing, China

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Abstract

A novel Gram-positive, rod-shaped, motile, spore-forming, nitrogen-fixing bacterium, designated strain 112T, was isolated from cabbage rhizosphere in Beijing, China. The strain was found to grow at 10–40 °C and pH 4–11, with an optimum of 30 °C and pH 7.0, respectively. Phylogenetic analysis based on a fragment of the full-length 16S rRNA gene sequence revealed that strain 112T is a member of the genus Paenibacillus. High levels of 16S rRNA gene similarities were found between strain 112T, Paenibacillus sabinae DSM 17841T (97.82 %) and Paenibacillus forsythiae DSM 17842T (97.22 %). However, the DNA–DNA hybridization values between strain 112T and the type strains of these two species were 10.36 and 6.28 %, respectively. The predominant menaquinone was found to be menaquinone 7 (MK-7). The major fatty acids were determined to be anteiso-C15:0 and C16:0. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and unknown aminophospholipids. The cell wall peptidoglycan was found to contain meso-diaminopimelic acid. The DNA G+C content was determined to be 55.4 mol%. On the basis of its phenotypic characteristics, 16S rRNA gene sequence analysis and the value of DNA–DNA hybridization, strain 112T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus brassicae sp. nov. is proposed. The type strain is 112T (= ACCC 01125T = DSM 24983T).

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No.30970083), and Special Fund for Agro-scientific Research in the Public Interest (201203045).

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Authors and Affiliations

  1. Ministry of Agriculture Key Laboratory of Crop Nutrition and Fertilization, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Miao Gao & Jian-guang Sun

  2. Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, School of Life Science, Ningxia University, Yinchuan, 750021, People’s Republic of China

    Hui Yang & Yu-jiong Wang

  3. School of Life Science, Neimenggu University, Huhehaote, 010021, People’s Republic of China

    Ji Zhao, Jun Liu & Yan-hua Sun

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  1. Miao Gao
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  2. Hui Yang
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  3. Ji Zhao
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  4. Jun Liu
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  5. Yan-hua Sun
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  7. Jian-guang Sun
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Correspondence to Jian-guang Sun.

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Gao, M., Yang, H., Zhao, J. et al. Paenibacillus brassicae sp. nov., isolated from cabbage rhizosphere in Beijing, China. Antonie van Leeuwenhoek 103, 647–653 (2013). https://doi.org/10.1007/s10482-012-9849-1

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  • DOI: https://doi.org/10.1007/s10482-012-9849-1

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