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Molecular genetic diversity of the Saccharomyces yeasts in Taiwan: Saccharomyces arboricola, Saccharomyces cerevisiae and Saccharomyces kudriavzevii

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Abstract

Genetic hybridization, sequence and karyotypic analyses of natural Saccharomyces yeasts isolated in different regions of Taiwan revealed three biological species: Saccharomyces arboricola, Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Intraspecies variability of the D1/D2 and ITS1 rDNA sequences was detected among S. cerevisiae and S. kudriavzevii isolates. According to molecular and genetic analyses, the cosmopolitan species S. cerevisiae and S. kudriavzevii contain local divergent populations in Taiwan, Malaysia and Japan. Six of the seven known Saccharomyces species are documented in East Asia: S. arboricola, S. bayanus, S. cerevisiae, S. kudriavzevii, S. mikatae, and S. paradoxus.

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Acknowledgments

We would like to thank J.P. Sampaio for kindly supplying Portuguese strains of S. kudriavzevii. This work was supported by a Russian-Taiwanese joint grant from Russian Foundation for Basic Research, Russia (No. 10-04-92008) and the National Science Council, Taiwan (NSC 99-2923-B-134-001-MY3). Synthesis of oligonucleotide primers and sequencing of the D1/D2 and ITS1 rDNA fragments were performed using the equipment of the Centre for Collective Use of GosNIIgenetika, having partial financial support from the Ministry of Education and Science of the Russian Federation (State Contract no. 16.552.11.7029).

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  1. State Institute for Genetics and Selection of Industrial Microorganisms, 1-Dorozhnyi 1, Moscow, 117545, Russia

    Gennadi I. Naumov & Elena S. Naumova

  2. Department of Applied Science, National Hsinchu University of Education, 521 Nanda Rd., Hsinchu, 30014, Taiwan

    Ching-Fu Lee

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  1. Gennadi I. Naumov
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  3. Elena S. Naumova
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Correspondence to Gennadi I. Naumov.

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Naumov, G.I., Lee, CF. & Naumova, E.S. Molecular genetic diversity of the Saccharomyces yeasts in Taiwan: Saccharomyces arboricola, Saccharomyces cerevisiae and Saccharomyces kudriavzevii . Antonie van Leeuwenhoek 103, 217–228 (2013). https://doi.org/10.1007/s10482-012-9803-2

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