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Magnetic Droplet Manipulation Platforms for Nucleic Acid Detection at the Point of Care

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Abstract

This review summarizes recent developments in the use of magnetically actuated droplets in point-of-care molecular diagnostic platforms. We discuss the fundamentals of magnetic droplet manipulation and the various modes of actuation. The balance of forces acting on a droplet during transport and particle extraction, as well as the devices and instrumentation developed to perform these operations will be presented and discussed. Furthermore, we review some of the recent advances on the diagnostic applications of platforms utilizing magnetic manipulation for genetic assessment of biological samples.

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Acknowledgments

The authors thank the funding support from NIH (R01CA155305, U54CA151838 and R21CA173390) and NSF (1159771 and 0967375).

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Authors and Affiliations

  1. Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA

    Dong Jin Shin & Tza-Huei Wang

  2. Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA

    Tza-Huei Wang

  3. Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD, USA

    Tza-Huei Wang

  4. Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA

    Tza-Huei Wang

  5. 108 Latrobe, 3400 N Charles St, Baltimore, MD, 21218, USA

    Tza-Huei Wang

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  1. Dong Jin Shin
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Correspondence to Tza-Huei Wang.

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Associate Editor Tingrui Pan oversaw the review of this article.

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Shin, D.J., Wang, TH. Magnetic Droplet Manipulation Platforms for Nucleic Acid Detection at the Point of Care. Ann Biomed Eng 42, 2289–2302 (2014). https://doi.org/10.1007/s10439-014-1060-2

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