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Effects of X-rays on Tuta absoluta for use in inherited sterility programmes

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Abstract

Tuta absoluta is a key pest of tomato crops originating from South America. The consequences of X-radiation on this species were studied under laboratory controlled conditions. The effect of radiation on adult emergence was evaluated exposing male and female pupae to increasing X-rays. Adult emergence decreased as doses of X-radiation increased, with the appearance of deformities such as malformed wings and bent legs at doses ≥350 Gy. Besides, males and females obtained from irradiated pupae were out crossed with untreated counterparts to explore the effects of X-radiation on inherited sterility. (a) Irradiated male × untreated female crosses. Both fecundity and fertility of the untreated females were reduced by radiation, and the effect was stronger as the doses increased. Neither the longevity of parental males and F1 adults nor the sex ratios of the F1 and F2 generations were affected by X-radiation (F1 and F2: first and second generation of descendants of irradiated adults). Inherited sterility effects were manifested by a significant reduction in the F1 fecundity, F1 fertility, and the amount of larvae and pupae produced. Doses of 200–250 Gy could be used to induce inherited sterility in T. absoluta males. (b) Untreated male × irradiated female crosses. The minimum dose at which irradiated females were completely sterile was 200 Gy. The present study is the first study in T. absoluta that provides the starting point for implementing the inherited sterility in this species.

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References

  • Agresti A (1996) An introduction to categorical data analysis. Wiley & Sons, Inc, University of Florida, USA

    Google Scholar 

  • Arthur V (2004) Use of gamma radiation to control three Lepidopteran pests in Brazil. Irradiation as a phytosanitary treatment of food and agricultural commodities. In: Proceedings of the final research coordination meeting organized by the joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, 2002, Vienna, Austria, pp 45–50

  • Ayvaz A, Albayrak S, Tunçbilek AS (2007) Inherited sterility in Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae): effect of gamma radiation on insect fecundity, fertility and developmental period. J Stored Prod Res 43:234–239

    Article  CAS  Google Scholar 

  • Bakri A, Mehta K, Lance DR (2005) Sterilizing insects with ionizing radiation. In: Dyck VA, Hendrichs J, Robinson AS (eds) Sterile insect technique. Principles and practice in area-wide integrated pest management. Springer, Dordrecht, pp 233–268

  • Bloem S, Carpenter JE (2001) Evaluation of population suppression by irradiated Lepidoptera and their progeny. Fla Entomol 84:165–170

    Article  Google Scholar 

  • Bloem S, Bloem KA, Carpenter JE, Calkins CO (1999) Inherited sterility in codling moth (Lepidoptera: Tortricidae): effect of substerilizing doses of radiation on insect fecundity, fertility and control. Ann Entomol Soc Am 92:222–229

    Google Scholar 

  • Bloem S, Bloem KA, Carpenter JE, Calkins CO (2001) Season-long releases of partially sterile males for control of codling moth, Cydia pomonella (Lepidoptera: Tortricidae), in Washington apples. Environ Entomol 30:763–769

    Article  Google Scholar 

  • Bloem S, Carpenter JE, Hofmeyr JH (2003) Radiation biology and inherited Sterility in false codling moth (Lepidoptera: Tortricidae). J Econ Entomol 96:1724–1731

    Article  PubMed  Google Scholar 

  • Boshra SA (2007) Effect of high-temperature pre irradiation on reproduction and mating competitiveness of male Sitotroga cerealella (Olivier) and their F1 progeny. J Stored Prod Res 43:73–78

    Article  Google Scholar 

  • Botto EN, Ceriani SA, López SN, Saini ED, Cedola CV, Segade G, Viscarret M (2000) Control biológico de plagas hortícolas en ambientes protegidos. La experiencia Argentina hasta el presente. RIA 29:83–98

    Google Scholar 

  • Carpenter JE, Gross HR (1993) Suppression of feral Heliothis zea (Lepidoptera: Noctuidae) populations following the infusion of inherited sterility from released substerile males. Environ Entomol 22:1084–1091

    Google Scholar 

  • Carpenter JE, Young JR, Sparks AN (1986) Fall armyworm (Lepidoptera: Noctuidae): comparison of inherited deleterious effects in progeny from irradiated males and females. J Econ Entomol 79:47–49

    Google Scholar 

  • Carpenter JE, Young JR, Sparks AN, Cromroy HL, Chowdhury MA (1987) Corn earworn (Lepidoptera: Noctuidae): effects off substerilizing doses of radiation and inherited sterility on reproduction. J Econ Entomol 80:483–489

    Google Scholar 

  • Carpenter JE, Bloem S, Bloem KA (2001) Inherited sterility in Cactoblastis cactorum (Lepidoptera: Pyralidae). Fla Entomol 84:537–542

    Article  Google Scholar 

  • Carpenter JE, Bloem S, Marec F (2005) Inherited sterility in insects. In: Dyck VA, Hendrichs J, Robinson AS (eds) Sterile insect technique principles and practice in area-wide integrated pest management, Chap 2.4. Springer, Netherlands, pp 115–146

  • de Coelho MCF, Franca FH (1987) Biologia, quetotaxia da larva e descricao da pupa e adulto da traca-do-tomateiro. Pesqui Agropecu Bras 22:129–135

  • de Siqueira HA, Guedes RN, Picanço MC (2000) Insecticide resistance in populations of Tuta absoluta (Lepidoptera:Gelechiidae). Agric Forest Entomol 2:147–153

    Article  Google Scholar 

  • Desneux N, Wajnberg E, Wyckhuys KAG, Burgio G, Arpaia S, Narváez-Vasquez CA, González-Cabrera J, Catalán Ruescas D, Tabone E, Frandon J, Pizzol J, Poncet C, Cabello T, Urbaneja A (2010) Biological invasion of European tomato crops by Tuta absoluta: ecology, geographic expansion and prospects for biological control. J Pest Sci 83:197–215

    Article  Google Scholar 

  • Desneux N, Luna MG, Guillemaud T, Urbaneja A (2011) The invasive South American tomato pinworm, Tuta absoluta, continues to spread in Afro-Eurasia and beyond: the new threat to tomato world production. J Pest Sci 84:403–408

    Article  Google Scholar 

  • Dhouibi MH, Abderahmane CT (2001) The effect of substerilizing doses of gamma radiation on the pupae of the carob moth Ectomyelois ceratoniae (Lepidoptera: Pyralidae). In: Proceedings of the symposium evaluation of population, suppression by irradiated Lepidoptera and their progeny. FAO/IAEA Final Research Coordination Meeting, 28–30 May 1998. Penang, Malaysia, IAEA-D4- RC-561, pp 43–48

  • Haddi K, Berger M, Bielza P, Cifuentes D, Field LM, Gorman K, Rapisarda C, Williamson MS, Bass C (2012) Identification of mutations associated with pyrethroid resistance in the voltage-gated sodium channel of the tomato leaf miner (Tuta absoluta). Insect Biochem Mol Biol 30:1–8

    Google Scholar 

  • Henneberry TJ, Clayton TE (1988) Effects of gamma radiation on pink bollworm (Lepidoptera: Gelechiidae) pupae: adult emergence, reproduction, mating, and longevity of emerged adults and their F1 progeny. J Econ Entomol 81:322–326

    Google Scholar 

  • Knipling EF (1955) Possibilities in insect control or eradication through the use of sexually sterile males. J Econ Entomol 48:459–462

    Google Scholar 

  • LaChance LE (1967) The induction of dominant lethal mutations in insects by ionizing radiation and chemicals—as related to the sterile-male technique of insect control In: Wright JW, Pal R (eds) Genetics of insect vectors of disease. Elsevier, Amsterdam, pp 617–650

  • LaChance LE (1985) Genetic methods for the control of the lepidopteran species: status and potential. U. S. Department of Agriculture, Washington, DC

  • Lietti MM, Botto E, Alsogaray RA (2005) Insecticide resistance in Argentine populations of Tuta absoluta (Meyrick) (Lepidoptera: Gelechidae). Neotrop Entomol 34:113–119

    Article  Google Scholar 

  • Makee H, Saour G (1997) Inherited effects in F1 progeny of partially sterile male Pthorimaea operculella (Lepidoptera: Gelechiidae). J Econ Entomol 90:1097–1101

    Google Scholar 

  • Makee H, Saour G (2003) Noninherited sterility in irradiated Pthorimaea operculella females. J Appl Entomol 127:489–493

    Article  Google Scholar 

  • Makee H, Tafesh N, Marec F (2008) Analysis of radiation-induced W chromosome aberrations in the codling moth, Cydia pomonella (L.), by Xuorescence in situ hybridization techniques. J Pest Sci 81:143–151

    Article  Google Scholar 

  • Mansour M (2010) Effects of gamma radiation on the Mediterranean flour moth, Ephestia kuehniella, eggs and acceptability of irradiated eggs by Trichogramma cacoeciae females. J Pest Sci 83:243–249

    Article  Google Scholar 

  • Marec F, Kollárová I, Pavelka J (1999) Radiation- induced inherited sterility combined with a genetic sexing system in Ephestia kuehniella (Lepidoptera: Pyralidae). Ann Entomol Soc Am 92:250–259

    Google Scholar 

  • Marec F, Tothová A, Sahara K, Traut W (2001) Meiotic pairing of sex chromosome fragments and its relation to atypical transmission of a sex-linked marker in Ephestia kuehniella (Insecta: Lepidoptera). Heredity 87:659–671

    Article  PubMed  CAS  Google Scholar 

  • Mastrangelo T, Parker AG, Jessup A, Pereira R, Orozco-Dávila D, Islam A, Dammalage T, Walder JMM (2010) A new generation of X ray irradiators for insect sterilization. J Econ Entomol 103:85–94

    Article  PubMed  CAS  Google Scholar 

  • Mastro VC (1993) Gypsy-moth F1 sterility program: current status. In: Proceedings of the symposium: radiation induced F1 sterility in Lepidoptera for area wide control. FAO/IAEA, 9-13 September 1991, Arizona, pp 125–129

  • Mehta K, Parker A (2011) Characterization and dosimetry of a practical X-ray alternative to self-shielded gamma irradiators. Radiat Phys Chem 80:107–113

    Article  CAS  Google Scholar 

  • Nguyen Thi QH, Nguyen Thanh TT (2001) Radiation-induced F1 sterility in Plutella xylostella (Lepidoptera: Plutellidae): potential for population suppression in the field. Fla Entomol 84:199–208

    Article  Google Scholar 

  • North DT (1975) Inherited sterility in Lepidoptera. Annu Rev Entomol 20:167–182

    Article  PubMed  CAS  Google Scholar 

  • North DT, Holt GG (1969) Population suppression by transmission of inherited sterility to progeny of irradiated cabbage loopers, Trichoplusia ni. Can Entomol 101:513–520

    Article  Google Scholar 

  • North DT, Holt GG (1971) Inherited sterility and its use in population suppression of Lepidoptera, pp 87–97. In: Proceedings of the symposium application of induced sterility for control of lepidoptera populations. FAO/IAEA, Viena, 1970

  • Ostergen G, Morris R, Wakoning T (1958) A study in Hyacinthus on chromosome size and breakability by X-rays. Hereditas 44:1–17

    Article  Google Scholar 

  • Pereyra PC, Sánchez NE (2006) Effect of two Solanaceous plants on developmental and population parameters of the tomato leaf miner, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Neotrop Entomol 35:671–676

    Article  PubMed  Google Scholar 

  • Proverbs MD (1962) Progress on the use of induced sexual sterility for the control of the codling moth Carpocapsa pomonella (L.) (Lepidoptera: Olethreutidae). P Entomol Soc Ont 92:5–11

    Google Scholar 

  • Proverbs MD, Newton JR, Logan DM (1978) Suppression of codling moth, Laspeyresia pomonella (Lepidoptera: Olethreutidae), by release of sterile and partially sterile moths. Can Entomol 110:1095–1102

    Article  Google Scholar 

  • Salazar ER, Araya JE (1997) Detección de resistencia a insecticidas en la polilla del tomate. Simiente 67:8–22

    Google Scholar 

  • Seth RK, Sharma VP (2001) Inherited sterility by substerilizing radiation in Spodoptera litura (Lepidoptera: Noctuidae): bio-efficacy and potential for pest suppression. Fla Entomol 84:183–193

    Article  Google Scholar 

  • Sparrow AH, Sparrow RC, Thompson KH, Schairer LA (1965) The use of nuclear and chromosomal variables in determining and predicting radiosensitivities. Radiat Bot 5:101–132

    Article  Google Scholar 

  • StatPoint (2011) Statistical software. StatPoint Technologies, Inc., Warrenton

  • StatSoft (2000) Statistica for Windows. Computer program manual. StatSoft Inc., Tulsa

  • Suomalainen E (1969) On the sex chromosome trivalent in some Lepidoptera females. Chromosoma 28:298–308

    Article  Google Scholar 

  • Sutrisno S, Hoedaya MS, Sutardi D, Rahayu A (1993) Radiation induced F1 sterility in diamondback moth, Plutella xylostella (L.), and tropical armyworm, Spodoptera lituria F. In: Proceedings of the symposium Radiation induced F1 sterility in Lepidoptera for area wide control. FAO/IAEA, 9–13 September 1991, Arizona, pp 23–36

  • Tobías CA (1952) The dependence of some biological effects of radiation on the rate of energy loss. In: Proceedings of the symposium on radiobiology. Wiley, New York, pp 357–392

  • Tothová A, Marec F (2001) Chromosomal principle of radiation-induced F1 sterility in Ephestia kuehniella (Lepidoptera: Pyralidae). Genome 44:172–184

    PubMed  Google Scholar 

  • Traut W, Marec F (1997) Sex chromosome differentiation in some species of Lepidoptera (Insecta). Chromosome Res 5:283–291

    Article  PubMed  CAS  Google Scholar 

  • Wolf KW (1996) The structure of condensed chromosomes in mitosis and meiosis of insects. Int J Insect Morphol Embryol 25:37–62

    Article  Google Scholar 

  • Wolf KW, Novák K, Marec F (1997) Kinetic organization of metaphase I bivalents in spermatogenesis of Lepidoptera and Trichoptera species with small chromosome numbers. Heredity 79:135–143

    Article  Google Scholar 

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Acknowledgments

We thank Ana María López for helpful comments on earlier version of our manuscript. We also thank Andrea Andorno and Carmen Hernández for technical support. Thanks to reviewers for their comments that helped us to improve our manuscript. This investigation received financial support from Agencia Nacional de Promoción Científica y Tecnológica (Argentina) and IAEA (International Atomic Energy Agency).

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Authors and Affiliations

  1. Insectario de Investigaciones para Lucha Biológica-IMYZA-CNIA-INTA Castelar, Las Cabañas y De los Reseros s/n C.C.25, 1712, Castelar, Buenos Aires, Argentina

    Cynthia L. Cagnotti, Mariana M. Viscarret, María B. Riquelme, Eduardo N. Botto & Silvia N. López

  2. Laboratorio de Genética de Insectos de Importancia Económica-IGEAF-CNIA-INTA Castelar, Nicolás Repetto y De los Reseros s/n C.C.25, 1712, Castelar, Buenos Aires, Argentina

    Leonela Z. Carabajal & Diego F. Segura

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  1. Cynthia L. Cagnotti
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  2. Mariana M. Viscarret
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  3. María B. Riquelme
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  4. Eduardo N. Botto
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  5. Leonela Z. Carabajal
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Correspondence to Cynthia L. Cagnotti.

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Communicated by N. Desneux.

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Cagnotti, C.L., Viscarret, M.M., Riquelme, M.B. et al. Effects of X-rays on Tuta absoluta for use in inherited sterility programmes. J Pest Sci 85, 413–421 (2012). https://doi.org/10.1007/s10340-012-0455-9

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  • DOI: https://doi.org/10.1007/s10340-012-0455-9

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