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Analysis of radiation-induced W chromosome aberrations in the codling moth, Cydia pomonella (L.), by fluorescence in situ hybridization techniques

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Abstract

The structure of the female sex-determining W chromosome was examined in wild-type females and in three mutant female lines of the codling moth, Cydia pomonella. The mutant lines were isolated from progeny of irradiated females using the abnormal appearance of sex chromatin body in female somatic polyploid nuclei as a cytogenetic marker. A detailed cytogenetic analysis was carried out using three different fluorescence in situ hybridization techniques: genomic in situ hybridization (GISH), comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH) with the codling moth W-chromosome painting probe. The FISH techniques enabled us to identify the W chromosome and its fragments in mitotic and meiotic chromosome complements and thus helped us to resolve complicated W chromosome aberrations induced by irradiation.

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References

  • Anisimov AI, Lazurkina NV, Shvedov AN (1989) Influence of radiation-induced genetic damage on the suppressive effect of inherited sterility in the codling moth (Lepidoptera: Tortricidae). Ann Entomol Soc Am 82:769–777

    Google Scholar 

  • Bloem S, Bloem KA, Carpenter JE, Calkins CO (1999) Inherited sterility in codling moth (Lepidoptera: Tortricidae): effect of sub-sterilising doses of radiation on field competitiveness. Environ Entomol 28:669–674

    Google Scholar 

  • Bloem S, Bloem KA, Carpenter JE, Calkins CO (2001) Season-long releases of partially sterile males for control of codling moth, Cydia pomonella (Lepidoptera: Tortricidae), in Washington apples. Environ Entomol 30:763–769

    Article  Google Scholar 

  • Dyck VA, Gardiner MGT (1992) Sterile-insect release program to control the codling moth Cydia pomonella (L.) (Lepidoptera, Olethreutidae) in British Columbia, Canada. Acta Phytopathol Entomol Hung 27:219–222

    Google Scholar 

  • Fuková I, Nguyen P, Marec F (2005) Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes. Genome 48:1083–1092

    Article  PubMed  Google Scholar 

  • Fukova I, Traut W, Vitkova M, Nguyen P, Kubickova S, Marec F (2007) Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin. Chromosoma 116:135–145 (Epub 2006)

    Article  PubMed  CAS  Google Scholar 

  • LaChance LE (1985) Genetic methods for the control of lepidopteran species: status and the potential. ARS-28, US Department of Agriculture, Washington DC, 40 pp

  • Makee H, Tafesh N (2006) Sex chromatin body as a marker of radiation-induced sex chromosome aberrations in the potato tuber moth, Phthorimaea operculella (Lepidoptera: Gelechiidae). J Pest Sci 79:75–82

    Article  Google Scholar 

  • Makee H, Tafesh N (2007) Sex chromatin body as a cytogenetic marker of W chromosome aberrations in Cydia pomonella females. In: Vreysen MJB, Robinson AS, Hendrichs J (eds) Area-wide control of insect pests. From research to field implementation. Springer, Dordrecht, pp 113–117

    Chapter  Google Scholar 

  • Marec F (1991) Genetic control of pest Lepidoptera: construction of a balanced lethal strain in Ephestia kuehniella. Entomol Exp Appl 61:271–283

    Article  Google Scholar 

  • Marec F, Mirchi R (1990) Genetic control of pest Lepidoptera: gamma-ray induction of translocations between sex chromosomes of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). J Stored Prod Res 26:109–116

    Article  Google Scholar 

  • Marec F, Traut W (1994) Sex chromosome pairing and sex chromatin bodies in W–Z translocation strains of Ephestia kuehniella (Lepidoptera). Genome 37:426–435

    Article  PubMed  CAS  Google Scholar 

  • Marec F, Neven LG, Robinson AS, Vreysen M, Goldsmith MR, Nagaraju J, Franz G (2005) Development of genetic sexing strains in Lepidoptera: from traditional to transgenic approaches. J Econ Entomol 98:248–259

    PubMed  Google Scholar 

  • Ohnuma A (1988) Synthesis of a balanced sex-linked lethal strain in silkworm (in Japanese). Reports of the Silk Science Research Institute vol 36, Tokyo, Japan, pp 17–25

  • Proverbs MD, Newton JR, Campbell CJ (1982) Codling moth—a pilot program of control by sterile insect release in British Columbia. Can Entomol 114:363–376

    Article  Google Scholar 

  • Savage JRK (2004) On the nature of visible chromosomal gaps and breaks. Cytogenet Genome 104:46–55

    Article  CAS  Google Scholar 

  • Strunnikov VA (1975) Sex control in silkworms. Nature 255:111–113

    Article  PubMed  CAS  Google Scholar 

  • Traut W (1976) Pachytene mapping in the female silkworm, Bombyx mori L. (Lepidoptera). Chromosoma 58:275–284

    Article  PubMed  CAS  Google Scholar 

  • Traut W, Marec F (1996) Sex chromatin in Lepidoptera. Q Rev Biol 71:239–256

    Article  PubMed  CAS  Google Scholar 

  • Traut W, Weith A, Traut G (1986) Structural mutants of the W chromosome in Ephestia (Insecta, Lepidoptera). Genetica 70:69–79

    Article  Google Scholar 

  • Traut W, Eickhof K, Schorch JC (2001) Identification and analysis of sex chromosomes by comparative genomic hybridization (CGH). Methods Cell Sci 23:157–163

    Article  Google Scholar 

  • Weith A, Traut W (1980) Synaptonemal complexes with associated chromatin in a moth, Ephestia kuehniella (L.). Chromsoma 78:275–291

    Article  Google Scholar 

  • Weith A, Traut W (1986) Synaptic adjustment, non-homolgous pairing of homologous segmentsin sex chromosome mutants of Ephestia kuehniella (Insecta, Lepidoptera). Chromsoma 94:125–131

    Article  Google Scholar 

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Acknowledgments

We wish to thank Dr. I.Othman and Dr. N. Mir Ali for their help and support; Ms. Hanady Haroon for technical assistance (The Atomic Energy Commission of Syria, Damascus, Syria), Ms. Iva Fuková for help with laboratory procedures, Ms. Marie Korchová for technical assistance (Institute of Entomology, Ceske Budejovice, Czech Republic), and Dr. A.I. Anisimov (Saint Petersburg, Russia) for introducing H. Makee to codling moth rearing in his lab. This work was funded by Research Contracts No. 10829/R given to H. Makee and No. 12055/R given to F.M. by the International Atomic Energy Agency, Vienna, Austria, and from the Entomology Institute project Z50070508. F. Marec acknowledges support from grants A6007307 of the Grant Agency of the Academy of Sciences of the Czech Republic (till 2005) and 206/06/1860 of the Grant Agency of the Czech Republic (since 2006), both Prague.

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Authors and Affiliations

  1. Atomic Energy Commission, P.O. Box 6091, Damascus, Syria

    H. Makee & N. Tafesh

  2. Biologicy Centre, ASCR, Institute of Entomology, and Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic

    F. Marec

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  1. H. Makee
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  2. N. Tafesh
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  3. F. Marec
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Correspondence to H. Makee.

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Communicated by M. Traugott.

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Makee, H., Tafesh, N. & Marec, F. Analysis of radiation-induced W chromosome aberrations in the codling moth, Cydia pomonella (L.), by fluorescence in situ hybridization techniques. J Pest Sci 81, 143–151 (2008). https://doi.org/10.1007/s10340-008-0199-8

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  • DOI: https://doi.org/10.1007/s10340-008-0199-8

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