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A New Stationary Phase for Analysis of Hydrophobic Compounds by RP-LC

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Abstract

Hydrophobic selectivity is a key factor that has made C18-bonded phases the main separation materials for HPLC since its development. However, hydrophobic compounds tend to be strongly retained in these phases meaning that large amounts of organic mobile phases are required. In this work, a new stationary phase was prepared from the immobilization of poly(dimethyl-co-alkylmethylsiloxane) (PDAS) over silica particles using microwave radiation. The optimized conditions for preparation of the stationary phase used 720 W of radiation for 10 min. The chromatographic performance and methylene selectivity in the separation of hydrophobic compounds by this new Si(PDAS) phase were similar to those obtained by typical C18-bonded phases. However, the lower hydrophobicity of the organic layer in the Si(PDAS) phase resulted in separation about 40 % faster than typical C18 phases, requiring more aqueous mobile phases. The Si(PDAS) phase showed lower silanophilic activity than that of typical C18 phases due to a more effective polymeric coating of the silica surface.

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Acknowledgments

The authors are thankful to the Instituto de Química at the Universidade Estadual de Campinas (UNICAMP, São Paulo, Brazil) for some spectroscopic characterizations and for the column packing. The authors thank FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for fellowships and financial support. This work is a collaboration research project of members of the Rede Mineira de Química (RQ-MG) supported by FAPEMIG (Project: CEX-RED-00010-14).

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Authors and Affiliations

  1. Química, Faculdade de Ciências Integradas do Pontal, Universidade Federal de Uberlândia, Ituiutaba, MG, 38304-402, Brazil

    Giselle O. Carvalho & Anizio M. Faria

  2. Instituto de Química, Universidade Estadual de Campinas, Campinas, SP, 13083-970, Brazil

    Carla G. A. da Silva

Authors
  1. Giselle O. Carvalho
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  2. Carla G. A. da Silva
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  3. Anizio M. Faria
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Correspondence to Anizio M. Faria.

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Carvalho, G.O., da Silva, C.G.A. & Faria, A.M. A New Stationary Phase for Analysis of Hydrophobic Compounds by RP-LC. Chromatographia 79, 19–27 (2016). https://doi.org/10.1007/s10337-015-2993-9

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  • DOI: https://doi.org/10.1007/s10337-015-2993-9

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