Abstract
A RP-LC method is presented, which is sensitive and selective for the simultaneous determination of enalapril–lercanidipine and enalapril–nitrendipine binary mixtures in their pharmaceutical dosage forms. The analyte peaks were detected using the LC method with the mobile phase ratio of methanol: water (70:30 v/v, pH 3.0) and a 1.0 mL min−1 flow rate. The detection wavelength was selected at 210 nm using photo diode array detector and column temperature was optimized to 30 °C. Linearity was obtained at different concentration ranges for all working pharmaceutically active compounds between 0.5 and 25 μg mL−1. The proposed methods were extensively validated according to USP 27 requirements and ICH guidelines. The methods were applied to the analysis of pharmaceutical dosage forms containing binary mixtures of enalapril–lercanidipine and enalapril–nitrendipine. Moreover, the proposed methods were applied for the degradation studies of the selected compounds. Degradation studies were conducted using stress conditions such as UV light, acidic and alkaline hydrolysis, oxidation and heat in oven, to evaluate the ability of the separation of the response of standard compounds from their degradation products.
Similar content being viewed by others
References
Frishman WH, Cheng-Lai A, Nawarskas J (2005) Current cardiovascular drugs, 4th edn. Current Medicine LLC, Philadelphia
Brown NJ, Vaughan DE (1998) Angiotensin-converting enzyme inhibitors. Circulation 97:1411–1420
Prisant ML (2005) Hypertension in the Elderly. Humana Press, New Jersey
Cocolas GH (1998) In: Delgado JN, Remers WA (eds) Textbook of organic medicinal and pharmaceutical chemistry, 10th edn. Lippincott-Raven, Philadelphia
Sweetman S (2009) Martindale: the complete drug reference, 36th edn. The Pharmaceutical Press, London
Sanborn KD (ed) (2007) Physicians Desk Reference, 61st edn. Medical Economics Company Inc., Montvale
Benedict CR (1999) Centrally acting antihypertensive drugs: re-emergence of sympathetic inhibition in the treatment of hypertension. Curr Hypertens Rep 1:305–312
McClellan KJ, Jarvis B (2000) Lercanidipine: a review of its use in hypertension. Drugs 60:1123–1140
Bianchi G, Passoni A, Griffini PL (1989) Effects of a new calcium antagonist, Rec 15/2375, on cardiac contractility of conscious rabbits. Pharmacol Res 21:193–200
Gilman AG, Goodman LS, Gilman AZ, Hardman JG, Molinoff PB, Limbird LE (1996) Goodman and Gillman’s the pharmacological basis of therapeutics. MacMillan, New York
Santiago TM, Lopez LM (1990) Nitrendipine: a new dihydropyridine calcium channel antagonist for the treatment of hypertension. Ann Pharmacother 4:167–175
Uslu B, Ozkan SA (2002) Determination of lamivudine and zidovudine in binary mixture using first derivative spectrophotometric, first derivative of the ratio spectra and HPLC-UV methods. Anal Chim Acta 466:175–185
Savaser A, Goraler S, Tasoz A, Uslu B, Lingeman H, Ozkan SA (2007) Simultaneous RP-LC Determination of Abacavir, Lamivudine and Zidovudine in pharmaceutical tablets, human serum and in drug dissolution studies. Chromatographia 65:259–265
Kurbanoglu S, Gumustas M, Ozkan SA (2013) Simultaneous estimation and validation of some binary mixtures of antihypertensive drugs by RP-LC methods using two new generation silica columns. J Pharm Biomed Anal 72:198–201
Caglayan MG, Palabiyik IM, Bor M, Onur F Optimisation and validation of liquid chromatographic and partial least-squares-1 methods for simultaneous determination of enalapril maleate and nitrendipine in pharmaceutical preparations. Chemical Papers doi:10.2478/s11696-011-0078-2
Gumustas M, Sanlı S, Sanlı N, Ozkan SA (2010) Determination of pKa values of some antihypertensive drugs by liquid chromatography and simultaneous assay of lercanidipine and enalapril in their binary mixtures. Talanta 82:1528–1537
El-Wasseef DR, El-Sherbiny DT, Abu El-Enin MA, El-Ashry SM (2011) Simultaneous in vitro HPLC determination of enalapril maleate and lercanidipine HCL. J Liq Chromatogr Rel Tech 34:48–60
Kaila HO, Ambasana MA, Thakkar RS, Saravaia HT, Shah AK (2011) A stability-indicating high performance liquid chromatographic assay for the simultaneous determination of atenolol and lercanidipine hydrochloride in tablets. Indian J Pharm Sci 73:376–380
Shang DW, Wang XP, Zhao XT, Huang FM, Tian GZ, Lu W, Zhou TY (2011) Simultaneous determination of nitrendipine and hydrochlorothiazide in spontaneously hypertensive rat plasma using HPLC with on-line solid-phase extraction. J Chromatogr B 879:3459–3464
Arayne MS, Sultana N, Tabassum A, Ali SN, Naveed S (2012) Simultaneous LC determination of rosuvastatin, lisinopril, captopril, and enalapril in API, pharmaceutical dosage formulations, and human serum. Med Chem Res 21:4542–4548
Thomas AB, Chaudhari AA, Nanda RK, Kothapalli LP, Chavan UB, Deshpande AD (2009) Simultaneous determination of Enalapril and Losartan in pharmaceutical preparations by UV spectrophotometry and LC. Chromatographia 69:1485–1487
Shetkar PB, Shinde VM (1997) Simultaneous determination of enalapril maleate and hydrochlorothiazide in tablets by reversed phase HPLC. Anal Lett 30:1143–1152
ICH GuidelinesTopic Q1AR (2000) Stability testing of new drug substances and products. International Conference on harmonization IFPMA, Geneva
Bliesner DM (2005) Validating chromatographic methods. Wiley, New Jersey
ICH Guidelines Topic Q2A (1995) Validation of analytical procedures, methodology. International Conference on Harmonization, Brussels, Belgium
The United States Pharmacopeia 27 (2004) The official compendia of standards. United States Pharmacopeial Convention, Rockville
Ozkan SA (2012) Electroanalytical methods in pharmaceutical analysis and their validation, 1st edn. HNB Pub, New York
Author information
Authors and Affiliations
Corresponding author
Additional information
Published in the topical collection New Developments for the Sensitive and Validated Drug Analysis Using Chromatographic Methods with guest editor Sibel A. Ozkan.
Rights and permissions
About this article
Cite this article
Kurbanoglu, S., Gumustas, M., Uslu, B. et al. A Sensitive and Selective RP-LC Method for the Simultaneous Determination of the Antihypertensive Drugs, Enalapril, Lercanidipine, Nitrendipine and Their Validation. Chromatographia 76, 1477–1485 (2013). https://doi.org/10.1007/s10337-013-2465-z
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10337-013-2465-z