Skip to main content
SpringerLink
Log in

A Generic High-Performance Liquid Chromatographic Method for Simultaneous Determination of Six Cardiovascular Drugs: Method Optimization and Application to Various Pharmaceutical Formulations

  • Published:
Pharmaceutical Chemistry Journal Aims and scope

Cardiovascular drugs are the main components of many formulations in marketplace. Herein, a fast high-performance liquid chromatographic assay was developed for the determination of pharmaceutical preparations containing antihypertensive agents including Atenolol (ATE), Hydrochlorothiazide (HCT), Ramipril (RAM), Amlodipine (AML), Losartan (LOS) and Telmisartan (TEL). Optimum separation was achieved using RP-C8 Waters Symmetry column (3.5 μm, 4.6 mm × 150 mm) and a mobile phase composed of 25 mM potassium phosphate buffer (pH 4.5 ± 0.1) and acetonitrile in gradient elution mode at 1.0 mL/min flow rate and 230 nm detection wavelength. Analysis was achieved in less than 9 min. The method was fully validated according to ICH guidelines. Linearity was achieved over the range of 10.0 – 60.0 μg/mL for ATE, LOS, Tel, 5.0 – 50.0 μg/mL for HCT, AML, and 1.0 – 11.0 μg/mL for RAM with acceptable accuracy, precision and limits of detection (< 2.0 μg/mL). Method robustness was assessed using small changes in buffer pH, acetonitrile ratio of the mobile phase, flow rate and detection wavelength. The proposed method was successfully applied to the analysis of their dosage forms combinations. Statistical comparison between the proposed and official methods was performed. The optimized method offers an economic chromatographic tool for the routine analysis of cardiovascular drugs in pharmaceutical quality control laboratories.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

References

  1. R. D. Howland, M. J. Mycek, R. A. Harvey, and P. C. Champe, Lippincott’s Illustrated Reviews: Pharmacology, Lippincott Williams & Wilkins, Philadelphia (2006).

    Google Scholar 

  2. S. C. Sweetman, Martindale : The Complete Drug Reference, Pharmaceutical Press, London (2009).

    Google Scholar 

  3. A. C. Moffat, M. D. Osselton, B.Widdop, and J.Watts, Clarke’s analysis of drugs and poisons, Pharmaceutical Press, London (2011), Vol. 3.

  4. K. K. Chaitanya, D. G. Sankar, and D. S. Israel, Global Treat. Pharm. Sci., 4, 1144 – 1152 (2013).

    CAS  Google Scholar 

  5. L. Li, C. Lai, X. Xuan, et al., J. Chromatogr. Sci., 54(8), 1415 – 1420 (2016).

    Article  CAS  PubMed  Google Scholar 

  6. P. S. Tungenwar, S. Ahmad, V. M. Shastry, and T. Mujawar, J. Pharm. BioSci., 5(2), 14 – 19 (2017).

    Article  CAS  Google Scholar 

  7. A. R. Tengli, B. Gurupadayya, and N. Soni, Int. J. Chem. Anal. Sci., 4(1), 33 – 38 (2013).

    Article  CAS  Google Scholar 

  8. M. Baing, V. Vaidya, R. Sane, et al., Chromatographia, 64(5), 293 – 296 (2006).

    Article  CAS  Google Scholar 

  9. D. D. Rao, N. V. Satyanarayana, S. S. Sait, et al., Chromatographia, 70(3 – 4), 647 – 651 (2009).

    Google Scholar 

  10. M. K. Shabana, D. Chapala, and P. S. Babu, Int. Res. J. Pharm., 4(8), 177 – 183 (2013).

    Article  Google Scholar 

  11. I. Salama, Bull. Fac. Pharm. Cairo Univ., 49(1), 19 – 24 (2011).

    CAS  Google Scholar 

  12. V. P. Kurade, M. G. Pai, and R. Gude, Indian J. Pharm. Sci., 71(2), 148 – 151 (2009).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. P. Wal, R. Tiwari, A. Wal, and G. Tiwari, Int. J. Appl. Pharm., 10(4), 51 (2018).

    Article  CAS  Google Scholar 

  14. R. Dubey and M. Ghosh, Sci. Pharm., 83(1), 107 – 124 (2015).

    Article  CAS  PubMed  Google Scholar 

  15. V. Gupta, R. Jain, O. Lukram, et al., Talanta, 83(3), 709 – 716 (2011).

    Article  CAS  PubMed  Google Scholar 

  16. S. Magiera, J. Chromatogr. B, 938, 86 – 95 (2013).

    Article  CAS  Google Scholar 

  17. A. K. Palakurthi, T. Dongala, and S. R. Yadlapalli, Sep. Sci. Plus, 3(6), 191 – 199 (2020).

    Article  CAS  Google Scholar 

  18. K. S. Lakshmi and S. Lakshmi, J. Anal. Methods Chem., 2012, 108281 (2012).

    PubMed  PubMed Central  Google Scholar 

  19. R. A. Shaalan, T. S. Belal, F. A. El Yazbi, and S. M. Elonsy, Bull. Fac. Pharm. Cairo Univ., 52(2), 225 – 237 (2014).

    Google Scholar 

  20. H. M. Ahmed, T. S. Belal, R. A. Shaalan, et al., Acta Chromatogr., 32(4), 219 – 227 (2020).

    Article  CAS  Google Scholar 

  21. A. Modroiu, G. Hancu, R. A. Vlad, et al., Curr. Issues Pharm. Med. Sci., 29, 42 – 46 (2016).

    Article  CAS  Google Scholar 

  22. British Pharmacopoeia, British Pharmacopoeial Commission, The Stationery Office, London (2016).

  23. ICH Harmonized Tripartite Guidelines, Validation of Analytical Procedures: Text and Methodology Q2(R1), International Conference on Harmonization of Technical Requirments for Registeration of Pharmaceuticals for Human Use, Geneva, (2005).

  24. S. Moldoveanu and V. David, Selection of HPLC Method in Chemical Analysis, Elsevier (2016).

  25. Validation of Chromatographic Methods, Center for Drug Evaluation and Research (CDER), Washington (1994).

  26. J. Nikelly, HPLC and CE: Principles and Practice, Academic Press, San Diego (1997).

Download references

Author information

Authors and Affiliations

  1. General Secretariat of Mental Health and Addiction Treatment, Cairo, Egypt

    Salma Saeed

  2. Analytical Chemistry Department, FacultyofPharmacy, Cairo University, Cairo, Egypt

    Ahmed H. Nadim, Ali M. Yehia & Azza A. Moustafa

  3. School of Life and Medical Sciences, University of Hertfordshire Hosted By Global Academic Foundation, New Capital, Garden City, Cairo, R5 New, Egypt

    Ali M. Yehia

Authors
  1. Salma Saeed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ahmed H. Nadim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ali M. Yehia
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Azza A. Moustafa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ahmed H. Nadim.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Saeed, S., Nadim, A.H., Yehia, A.M. et al. A Generic High-Performance Liquid Chromatographic Method for Simultaneous Determination of Six Cardiovascular Drugs: Method Optimization and Application to Various Pharmaceutical Formulations. Pharm Chem J 57, 138–145 (2023). https://doi.org/10.1007/s11094-023-02861-w

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11094-023-02861-w

Keywords

Search

Navigation