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Purification, characterization, and application of a thermostable dextranase from Talaromyces pinophilus

  • Biotechnology Methods - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Dextranase can hydrolyze dextran to low-molecular-weight polysaccharides, which have important medical applications. In the study, dextranase-producing strains were screened from various soil sources. The strain H6 was identified as Talaromyces pinophilus by a standard ITS rDNA analysis. Crude dextranase was purified by ammonium sulfate fractionation and Sepharose 6B chromatography, which resulted in a 6.69-fold increase in the specific activity and an 11.27% recovery. The enzyme was 58 kDa, lower than most dextranase, with an optimum temperature of 45 °C and an optimum pH of 6.0, and identified as an endodextranase. It was steady over a pH range from 3.0 to 10.0 and had reasonable thermal stability. The dextranase activity was increased by urea, which enhanced its activity to 115.35% and was conducive to clinical dextran production. Therefore, T. pinophilus H6 dextranase could show its superiority in practical applications.

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Acknowledgements

The study was supported by the National Natural Science Foundation of China (Grant No. 81573399).

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Authors and Affiliations

  1. Department of Pharmaceutical Engineering, School of Biological and Medical Engineering, Hefei University of Technology, Hefei, 230009, People’s Republic of China

    Yu-Qi Zhang, Ruo-Han Li, Hong-Bin Zhang, Min Wu & Xue-Qin Hu

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  1. Yu-Qi Zhang
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  2. Ruo-Han Li
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  3. Hong-Bin Zhang
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Correspondence to Hong-Bin Zhang.

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Zhang, YQ., Li, RH., Zhang, HB. et al. Purification, characterization, and application of a thermostable dextranase from Talaromyces pinophilus . J Ind Microbiol Biotechnol 44, 317–327 (2017). https://doi.org/10.1007/s10295-016-1886-8

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  • DOI: https://doi.org/10.1007/s10295-016-1886-8

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