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Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method

  • Environmental Microbiology
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Sugarcane ethanol production occurs in non-sterile conditions, and microbial contamination can decrease productivity. In this study, we assessed the microbial diversity of contaminants of ethanol production in an industrial facility in Brazil. Samples obtained at different stages were analyzed by pyrosequencing-based profiling of bacterial and archaeal 16S rRNA genes and the fungal internal transcribed spacer region. A total of 355 bacterial groups, 22 archaeal groups, and 203 fungal groups were identified, and community changes were related to temperature changes at certain stages. After fermentation, Lactobacillus and unclassified Lactobacillaceae accounted for nearly 100 % of the bacterial sequences. Predominant Fungi groups were “unclassified Fungi,” Meyerozyma, and Candida. The predominant Archaea group was unclassified Thaumarchaeota. This is the first work to assess the diversity of Bacteria, and Archaea and Fungi associated with the industrial process of sugarcane-ethanol production using culture-independent techniques.

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Acknowledgments

O.Y.A.C. was supported by a CNPq fellowship. This work was supported by grant from CNPq, FAD-DF, Embrapa.

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Authors and Affiliations

  1. Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, Brasília, DF, 70790-160, Brazil

    Ohana Yonara Assis Costa, Daiva Domenech Tupinambá, Jessica Carvalho Bergmann, Cristine Chaves Barreto & Betania Ferraz Quirino

  2. Embrapa-Agroenergy, Parque Estação Biológica, s/n, Brasília, DF, 70770-901, Brazil

    Betulia Morais Souto & Betania Ferraz Quirino

  3. Department of Cellular Biology, Universidade de Brasilia, Brasília, DF, 70910-900, Brazil

    Cynthia Maria Kyaw & Ricardo Henrique Kruger

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  1. Ohana Yonara Assis Costa
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Costa, O.Y.A., Souto, B.M., Tupinambá, D.D. et al. Microbial diversity in sugarcane ethanol production in a Brazilian distillery using a culture-independent method. J Ind Microbiol Biotechnol 42, 73–84 (2015). https://doi.org/10.1007/s10295-014-1533-1

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