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Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine

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Abstract

The aim of this study was to investigate the effect of laser photobiomodulation (PBM) on the viability of the transverse rectus abdominis musculocutaneous (TRAM) flap in rats subjected to the action of nicotine. We evaluated 60 albino Wistar rats, divided into six groups of ten animals. Group 1 (saline) underwent the surgical technique to obtain a TRAM flap; group 2 (laser 830 nm) underwent the surgical technique and was irradiated with a laser 830 nm; group 3 (laser 660 nm) underwent the surgical technique and was irradiated with a laser 660 nm; group 4 was treated with nicotine subcutaneously (2 mg/kg/2×/day/4 weeks) and underwent surgery; group 5 (nicotine + laser 830 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 830 nm; group 6 (nicotine + laser 660 nm) was exposed to nicotine, underwent the surgical technique, and was irradiated with a laser 660 nm. The application of PBM occurred immediately after surgery and on the two following days. The percentage of necrosis was assessed using the AxioVision® software. The number of mast cells (toluidine blue staining) was evaluated, and immunohistochemistry was performed to detect vascular endothelial growth factor expression (anti-VEGF-A), fibroblasts (anti-basic FGF), and neoformed vessels (anti-CD34). PBM with a wavelength of 830 nm increased the viability of the TRAM flap, with a smaller area of necrosis, increased number of mast cells, and higher expression of VEGF and CD34. PBM increases the viability of musculocutaneous flaps treated with to nicotine.

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Authors and Affiliations

  1. Post-Graduate Program in Rehabilitation and Functional Performance of Ribeirão Preto Medical School of the University of São Paulo (FMRP/USP), Av. dos Bandeirantes, 3900, Ribeirão Preto, SP, 14049-900, Brazil

    Lais Mara Siqueira das Neves, João Eduardo de Araújo & Elaine Caldeira de Oliveira Guirro

  2. Post-Graduate Program in Rehabilitation Sciences, Federal University of Santa Catarina - Campus Araranguá – UFSC/SC, Florianópolis, Brazil

    Gabriella de Paula Marcondes Ferreira Leite & Alexandre Marcio Marcolino

  3. Post-Graduate Program in Interdisciplinary Health Sciences, Federal University of Sao Paulo - Santos Campus - UNIFESP/SP, Sao Paulo, Brazil

    Carlos Eduardo Pinfildi

  4. Post-Graduate Program in Pathology, Ribeirão Preto Medical School – FMRP/USP, Sao Paulo, Brazil

    Sérgio Britto Garcia

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  1. Lais Mara Siqueira das Neves
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  2. Gabriella de Paula Marcondes Ferreira Leite
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  3. Alexandre Marcio Marcolino
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  4. Carlos Eduardo Pinfildi
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  6. João Eduardo de Araújo
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  7. Elaine Caldeira de Oliveira Guirro
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Corresponding author

Correspondence to Elaine Caldeira de Oliveira Guirro.

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Financial support

This study was funded by Foundation of Sao Paulo State Research (FAPESP) Grants 2013/07602-6 and 2013/07732-7.

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

This study was approved by the Ethics Committee for the Use of Animals (CEUA) at the Faculty of Medicine of Ribeirão Preto/University of São Paulo, under protocol number 46/2013.

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das Neves, L.M.S., Leite, G.d.P.M.F., Marcolino, A.M. et al. Laser photobiomodulation (830 and 660 nm) in mast cells, VEGF, FGF, and CD34 of the musculocutaneous flap in rats submitted to nicotine. Lasers Med Sci 32, 335–341 (2017). https://doi.org/10.1007/s10103-016-2118-1

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  • DOI: https://doi.org/10.1007/s10103-016-2118-1

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