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Low-level laser therapy (670 nm) on viability of random skin flap in rats

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Abstract

This study investigated the effects of 670 nm laser, at different fluences, on the viability of skin flap in rats. One hundred male animals were used. The animals were divided into control group; group treated with 3 J/cm2; group treated with 6 J/cm2; group treated with 12 J/cm2 and group treated with 24 J/cm2. The skin flap was made on the backs of all animals studied, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was done immediately after the surgery and on days 1, 2, 3 and 4 after surgery. The percentage of necrosis of the flap was calculated at the 7th postoperative day. Additionally, a sample of each flap was collected to enable us to count the blood vessels. Treated animals showed a statistically significant smaller area of necrosis than did the control group. The necrosis in the treated groups was 41.82% (group 2), 36.51% (group 3), 29.45% (group 4) and 20.37% (group 5). We also demonstrated that laser irradiation at 670 nm, at all doses used, had a stimulatory effect on angiogenesis. Our study showed that the 670 nm laser was efficient to increase the viability of the skin flap, at all fluences used, with a tendency of reaching better results at higher doses.

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Authors and Affiliations

  1. Department of Physiotherapy, Federal University of São Carlos, Sao Carlos, SP, Brazil

    Paulo Sérgio Bossini, Renan Fangel, Rafael Malfará Habenschus & Nivaldo Antonio Parizotto

  2. Department of Health Science, Federal University of Sao Paulo, Santos, SP, Brazil

    Ana Claudia Renno

  3. Department of Statistic, Federal University of São Carlos, Sao Carlos, SP, Brazil

    Benedito Benze

  4. Department of Physiology and Pathology, Estadual University of the State of Sao Paulo (UNESP), Araraquara, SP, Brazil

    José Antônio Zuanon & Carlos Benatti Neto

  5. Department of Physiotherapy, Federal University of São Paulo, Av. Ana Costa, 95, Santos, SP, 15011-000, Brazil

    Ana Claudia Renno

Authors
  1. Paulo Sérgio Bossini
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  2. Renan Fangel
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  3. Rafael Malfará Habenschus
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  4. Ana Claudia Renno
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  5. Benedito Benze
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  8. Nivaldo Antonio Parizotto
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Correspondence to Ana Claudia Renno.

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Bossini, P.S., Fangel, R., Habenschus, R.M. et al. Low-level laser therapy (670 nm) on viability of random skin flap in rats. Lasers Med Sci 24, 209–213 (2009). https://doi.org/10.1007/s10103-008-0551-5

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  • DOI: https://doi.org/10.1007/s10103-008-0551-5

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