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Identification and characterization of 2-keto-3-deoxy-l-rhamnonate dehydrogenase belonging to the MDR superfamily from the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans: implications to l-rhamnose metabolism in archaea

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Abstract

We identified the non-phosphorylated l-rhamnose metabolic pathway (Rha_NMP) genes that are homologous to those in the thermoacidophilic archaeon Thermoplasma acidophilum in the genome of the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans. However, unlike previously known 2-keto-3-deoxy-l-rhamnonate (l-KDR) dehydrogenase (KDRDH) which belongs to the short chain dehydrogenase/reductase superfamily, the putative KDRDHs in S. thermosulfidooxidans (Sulth_3557) and T. acidophilum (Ta0749) belong to the medium chain dehydrogenase/reductase (MDR) superfamily. We demonstrated that Sulth_3559 and Sulth_3557 proteins from S. thermosulfidooxidans function as l-rhamnose dehydrogenase and KDRDH, respectively. Sulth_3557 protein is an NAD+-specific KDRDH with optimal temperature and pH of 50 °C and 9.5, respectively. The K m and V max values for l-KDR were 2.0 mM and 12.8 U/mg, respectively. Sulth_3557 also showed weak 2,3-butanediol dehydrogenase activity. Phylogenetic analysis suggests that Sulth_3557 and its homologs form a new subfamily in the MDR superfamily. The results shown in this study imply that thermoacidophilic archaea metabolize l-rhamnose to pyruvate and l-lactate by using the MDR-family KDRDH similarly to that of the thermoacidophilic bacterium S. thermosulfidooxidans.

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Acknowledgments

This work was supported by the Marine Biotechnology Program of Korean Ministry of Land, Transport and Maritime Affairs and the BK21 Program of Korean Ministry of Education.

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Authors and Affiliations

  1. Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, 790-784, Korea

    Jungdon Bae, Suk Min Kim & Sun Bok Lee

  2. Graduate School of Engineering Mastership, Pohang University of Science and Technology, San 31, Hyoja Dong, Pohang, 790-784, Korea

    Sun Bok Lee

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  1. Jungdon Bae
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  2. Suk Min Kim
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Correspondence to Sun Bok Lee.

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Communicated by A. Oren.

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Bae, J., Kim, S.M. & Lee, S.B. Identification and characterization of 2-keto-3-deoxy-l-rhamnonate dehydrogenase belonging to the MDR superfamily from the thermoacidophilic bacterium Sulfobacillus thermosulfidooxidans: implications to l-rhamnose metabolism in archaea. Extremophiles 19, 469–478 (2015). https://doi.org/10.1007/s00792-015-0731-8

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