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Occurrence and distribution of capB in Antarctic microorganisms and study of its structure and regulation in the Antarctic biodegradative Pseudomonas sp. 30/3

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Abstract

The analysis of the cold-shock domain (CSD)-encoding genes, capB and cspA, by PCR amplification showed presence of capB in all 18 Antarctic Pseudomonas isolates, but the absence of cspA. Nucleotide sequence analysis of capB ORF from a biodegradative Pseudomonas 30/3 and its regulatory sequences including the promoter and 5′-UTR was determined and compared with the other CSD-encoding genes. Expression analysis using translational gene fusion of the putative capB promoter and its flanking sequence from Pseudomonas sp. 30/3 with lacZ′ exhibited a significant increase in β-galactosidase activity at 15 and 6°C. Unlike the expression of E. coli CspA, Pseudomonas sp. 30/3 showed a slow but steady increase of the CapB expression at 6°C. Subcellular localization of CapB at 6°C showed accumulation in and around the nucleoid whereas at 22 or 30°C, it was identified around the nucleoid as well as in the cytosol. Our study attempts to elucidate the detailed structure of capB from Pseudomonas 30/3 and the role of 5′UTR in the transcriptional regulation along with the possible role of CapB in transcription and translation suited for the cold adaptation of this bacterium in Antarctic environment.

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Acknowledgments

This study was supported in part the UAB Faculty Development award and the Department of Biology, and the Foundation of Research, Science and Technology, New Zealand (C09X0018). The logistic support was provided by Antarctica New Zealand; 2008 Tawani International Scientific Expedition (Tawani Foundation, Chicago, IL); and Antarctic Maitri (NCAOR, India) and Novolazarevskaya (Russia) stations. We thank Col (Ret) James Pritzker for supporting the Tawani Expedition; Rasik Ravindra (Director, NCAOR, India), Cdr. Arun Chaturvedi, Cdr. Pradip Malhotra and Ashit Swain for field support. We thank Edward Phillips at UAB High Resolution Imaging Shared Facility for the fluorescent microscopy study.

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Author notes

  1. Gitika Panicker

    Present address: Center for Disease Control and Prevention, Atlanta, GA, USA

  2. Teruaki Nakatsuji

    Present address: VA Medical Center, University of California at San Diego, San Diego, CA, USA

Authors and Affiliations

  1. Department of Biology, University of Alabama at Birmingham, 1300 University Boulevard, CH103, Birmingham, AL, 34294-1170, USA

    Gitika Panicker, Nazia Mojib, Teruaki Nakatsuji & Asim K. Bej

  2. Landcare Research, Private Bag 3127, Hamilton, New Zealand

    Jackie Aislabie

  3. Department of Biology, University of Alabama at Birmingham, 1300 University Blvd., CH103, Birmingham, AL, 35294-1170, USA

    Asim K. Bej

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  1. Gitika Panicker
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Correspondence to Asim K. Bej.

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Communicated by A. Oren.

G. Panicker and N. Mojib contributed equally to this paper.

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Panicker, G., Mojib, N., Nakatsuji, T. et al. Occurrence and distribution of capB in Antarctic microorganisms and study of its structure and regulation in the Antarctic biodegradative Pseudomonas sp. 30/3. Extremophiles 14, 171–183 (2010). https://doi.org/10.1007/s00792-009-0296-5

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