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Complete genomic characterization of milk vetch dwarf virus isolates from cowpea and broad bean in Anhui province, China

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Abstract

Cowpea and broad bean plants showing severe stunting and leaf rolling symptoms were observed in Hefei city, Anhui province, China, in 2014. Symptomatic plants from both species were shown to be infected with milk vetch dwarf virus (MDV) by PCR. The complete genomes of MDV isolates from cowpea and broad bean were sequenced. Each of them had eight genomic DNAs that differed between the two isolates by 10.7% in their overall nucleotide sequences. In addition, the MDV genomes from cowpea and broad bean were associated with two and three alphasatellite DNAs, respectively. This is the first report of MDV on cowpea in China and the first complete genome sequences of Chinese MDV isolates.

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Acknowledgements

We thank Prof. M. J. Adams, Stevenage, Herts, UK, for correcting the English in the manuscript.

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Authors and Affiliations

  1. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Chenhua Zhang & Jianping Chen

  2. State Key Laboratory Breeding Base for Sustainable Control of Plant Pest and Disease, Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang Province, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China

    Chenhua Zhang, Hongying Zheng, Kelei Han, Xijiao Song, Jianping Chen & Fei Yan

  3. Institute of Plant Protection and Agricultural Product Quality and Safety, Anhui Academy of Agricultural Sciences, Hefei, 230031, China

    Dankan Yan & Dongfang Zhang

  4. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, 410425, China

    Yong Liu

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  1. Chenhua Zhang
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  2. Hongying Zheng
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  3. Dankan Yan
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  4. Kelei Han
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  7. Dongfang Zhang
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  9. Fei Yan
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Corresponding authors

Correspondence to Dongfang Zhang, Jianping Chen or Fei Yan.

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This article does not contain any studies with human participants or animals performed by any of the authors and is in compliance with ethical standards for research.

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The authors declare that they have no conflict of interest.

Funding

This study was funded by Special Fund for Agro-Scientific Research in the Public Interest (grant number: 201303028) and the International Science & Technology Cooperation Program of China (grant number: 2015DFA30700).

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Zhang, C., Zheng, H., Yan, D. et al. Complete genomic characterization of milk vetch dwarf virus isolates from cowpea and broad bean in Anhui province, China. Arch Virol 162, 2437–2440 (2017). https://doi.org/10.1007/s00705-017-3348-7

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  • DOI: https://doi.org/10.1007/s00705-017-3348-7

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