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Identification of further diversity among posaviruses

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Abstract

Recently, there have been reports of new members of posavirus-like viruses in the order Picornavirales. In this study, using a metagenomics approach, 11 posavirus-like sequences (>7,000 nucleotides) were detected in 155 porcine fecal samples. Phylogenetic analysis revealed that the newly identified virus sequences, together with other posavirus-like viruses, form distinct clusters within the order Picornavirales, composed of eight genogroups and unassigned sequences based on amino acid sequences of the helicase and RNA-dependent RNA polymerase regions, with <40 % and <50 % sequence identity, respectively. We propose further classifications of highly diverse posavirus populations based on newly identified sequences from Japanese pig feces.

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Acknowledgments

This work was supported by grants from the Ministry of Health, Labour and Welfare of Japan and JSPS KAKENHI grant number 15K07718.

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    Authors and Affiliations

    1. Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan

      Kaori Sano, Yuki Naoi, Mai Kishimoto, Shinobu Tsuchiaka, Tsutomu Omatsu, Tetsuya Furuya, Yukie Katayama, Mami Oba, Junsuke Shirai, Tetsuya Mizutani & Makoto Nagai

    2. Kurayoshi Livestock Hygiene Service Center, Kurayoshi, Tottori, 683-0017, Japan

      Tsuneyuki Masuda & Hiroshi Yamasato

    3. Rokko Livestock Hygiene Service Center, Hokota, Ibaraki, 311-1593, Japan

      Hitomi Tanabe

    4. Ishikawa Nanbu Livestock Hygiene Service Center, Kanazawa, Ishikawa, 920–3101, Japan

      Mika Ito

    5. Tochigi Prefectural South District Animal Hygiene Service Center, Tochigi, Tochigi, 328-0002, Japan

      Kazutaka Niira

    6. Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, 208-0011, Japan

      Kei Haga & Kazuhiko Katayama

    7. Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan

      Keigo Asano, Motohiko Ishida & Makoto Nagai

    8. Kenhoku Livestock Hygiene Service Center, Mito, Ibaraki, 310-0002, Japan

      Yoshinao Ouchi

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    1. Kaori Sano
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    2. Yuki Naoi
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    Corresponding author

    Correspondence to Makoto Nagai.

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    K. Sano, Y. Naoi and M. Kishimoto contributed equally to this work.

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    Sano, K., Naoi, Y., Kishimoto, M. et al. Identification of further diversity among posaviruses. Arch Virol 161, 3541–3548 (2016). https://doi.org/10.1007/s00705-016-3048-8

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    • DOI: https://doi.org/10.1007/s00705-016-3048-8

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