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Reproduction biology and chloroplast inheritance in Bromeliaceae: a case study in Fosterella (Pitcairnioideae)

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Abstract

We applied a series of intra- and interspecific in situ cross-pollination experiments under greenhouse conditions to evaluate the breeding systems in four Fosterella species (Pitcairnioideae s.str.; Bromeliaceae). Viable hybrids were produced between each pair of the investigated species, suggesting that reproduction barriers may be low also under natural conditions. Seed germination rates proved to be high in each crossing treatment, indicating a high viability of the artificial hybrids. Large numbers of seeds were produced after both closed and open pollination treatments, suggesting that autogamy may be a major reproductive strategy in the genus. Our results support the concept that self-compatibility is an appropriate way to avoid natural hybridization in Bromeliaceae and could assist in maintaining species integrity in the presence of pollen flow. Paternity was verified in all crosses by genotyping parents and offspring with a set of polymorphic nuclear microsatellite markers. To study the mode of chloroplast inheritance, we developed a novel set of 24 chloroplast microsatellite markers using 454 pyrosequencing technology, and applied four of these markers for genotyping parents and offspring from all crosses. Our results clearly demonstrated a maternal inheritance of plastids.

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Acknowledgments

Special thanks are due to the gardener team of the University of Kassel for their professional cultivation of Fosterella plants and their help during the crossing experiments and the growing of seedlings. The plant material used in the present study was kindly provided by the Botanical Gardens of Berlin, Frankfurt, Hannover, Heidelberg, Kiel (all Germany), Dr. Nicole Schütz (Stuttgart State Museum of Natural History, Germany), Dr. Jule Peters (University of Kassel), Dr. Ivón Ramírez-Morillo (Centro de Investigación Científica de Yucatán, Mexico) and Christoph Nowicki (University of Applied Sciences, Eberswalde). We thank Dr. Bruno Huettel from the Max-Planck Genome Centre Cologne for supplying the primary 454 sequence data of Fosterella rusbyi. We appreciated the valuable comments given by two anonymous reviewers on an earlier version of the manuscript.

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    1. Department of Sciences, Plant Molecular Systematics, University of Kassel, Heinrich-Plett-Str. 40, 34132, Kassel, Germany

      Natascha D. Wagner, Tina Wöhrmann, Veronika Öder, Avigdor Burmeister & Kurt Weising

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    1. Natascha D. Wagner
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    Handling editor: Christoph Oberprieler.

    N. D. Wagner and T. Wöhrmann contributed equally to this work and are considered as joint first authors.

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    Wagner, N.D., Wöhrmann, T., Öder, V. et al. Reproduction biology and chloroplast inheritance in Bromeliaceae: a case study in Fosterella (Pitcairnioideae). Plant Syst Evol 301, 2231–2246 (2015). https://doi.org/10.1007/s00606-015-1226-x

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