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Shoot organogenesis from roots of seabuckthorn (Hippophaë rhamnoides L.): structure, initiation and effects of phosphorus and auxin

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Abstract

Key message

Shoots from roots formed on seedlings in vitro in the groove of lateral roots as on plants growing in a natural habitat. The finding has ecological and applied relevance.

Abstract

Shoot organogenesis from roots (root suckers, shoots from roots, SfRs) allows vegetative propagation of the small tree seabuckthorn (Hippophaë rhamnoides L.) in addition to sexual propagation and is an important trait associated with the root system. Using an in vitro system, we studied initiation, localization and development of SfRs and interacting roles of phosphorus (P) and indole-3-acetic acid (IAA). After transfer of seedlings to the W4 medium (WPM medium with added IAA, benzyl adenine and giberellic acid), SfRs protruded after 2 weeks from the primary root initially at the groove of lateral roots (LR) as seen by scanning electron microscopy and light microscopy. This is also the location of SfRs on plants growing in natural conditions, which suggests a similar developmental pathway. To localize SfR initiation, staining by the DNA-binding fluorochrome 4′,6-diamidino-2-phenylindole in root cross sections revealed a high density of small cells as in meristems in the pericycle area between endodermis and vascular tissue. During 8–10 weeks, SfRs emerged also in other positions on the primary root, concomitantly with senescence of existing LRs and suppression of new LR formation. Since SfR formed in relation to LRs, we hypothesized that P and IAA play a role in SfR formation. Highest production of SfRs occurred in W4 medium after pre-treatment with high P in the presence of IAA while LR production in WPM was stimulated by IAA in low and middle P. SfRs developed in vitro on seedlings originating from three subspecies of H. rhamnoides showing this trait to be common. This experimental system allowed studies of organogenesis of SfRs and LRs in response to plant growth regulators, P and IAA and may be further implemented in basic studies and in applied clonal propagation of seabuckthorn.

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Abbreviations

BA:

Benzyl adenine

DAPI:

4′,6-Diamidino-2-phenylindole

GA:

Gibberrelic acid (GA3)

IAA:

Indole-3-acetic acid

LR:

Lateral root

PGR:

Plant growth regulator

PM:

Plain medium, agar-phytagel in water

SfR:

Shoot-from-root

WPM:

Woody plant medium

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Acknowledgments

A PhD scholarship to SRUS from the Higher Education Commission, Pakistan through Lasbela University of Agriculture Water and Marine Sciences, Uthal, Pakistan and a postdoctoral scholarship to TP from the Swedish Institute are gratefully acknowledged. Funding from Stiftelsen Oscar och Lili Lamms Minne and Formas, the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning to P-OL is gratefully acknowledged. We thank Galina Timermane for supplying seeds of cv. Sunny and cv. Gold Rain, and Guo Hai for seeds of H. r. ssp sinensis. We thank Natalia Demidova, Northern Research Institute of Forestry, Russia, and Kimmo Rumpunen, Swedish University of Agricultural Sciences, Sweden, for providing information regarding cvs. BHi10726 and BHi32415.

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  1. Tatiana Plaksina

    Present address: The Lisavenko Research Institute of Horticulture for Siberia, 49 Zmeinogorskiy Tract, Barnaul, Russia

Authors and Affiliations

  1. Department of Plant Biology, Uppsala BioCenter, Linnean Centre of Plant Biology in Uppsala, Swedish University of Agricultural Sciences, PO Box 7080, 750 07, Uppsala, Sweden

    Syed Rehmat Ullah Shah, Tatiana Plaksina, Sridevy Sriskandarajah & Per-Olof Lundquist

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  1. Syed Rehmat Ullah Shah
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Correspondence to Per-Olof Lundquist.

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Shah, S.R.U., Plaksina, T., Sriskandarajah, S. et al. Shoot organogenesis from roots of seabuckthorn (Hippophaë rhamnoides L.): structure, initiation and effects of phosphorus and auxin. Trees 29, 1989–2001 (2015). https://doi.org/10.1007/s00468-015-1278-0

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