Human Genetics

, Volume 133, Issue 4, pp 403–416

Recollections of a scientific journey published in human genetics: from chromosome territories to interphase cytogenetics and comparative genome hybridization

Review Paper

DOI: 10.1007/s00439-014-1425-5

Cite this article as:
Cremer, T., Cremer, C. & Lichter, P. Hum Genet (2014) 133: 403. doi:10.1007/s00439-014-1425-5

Abstract

In line with the intentions of an issue celebrating the 50th anniversary of Human Genetics, we focus on a series of frequently cited studies published in this journal during the 1980s and 1990s. These studies have contributed to the rise of molecular cytogenetics. They yielded evidence that chromosomes occupy distinct territories in the mammalian cell nucleus, first obtained with laser-UV-microbeam experiments and thereafter with chromosome painting, and contributed to the development of interphase cytogenetics and comparative genome hybridization. We provide a personal account of experimental concepts, which were developed by us and others, and describe some of the unforeseeable turns and obstacles, which we had to overcome on the way towards an experimental realization. We conclude with a perspective on current developments and goals of molecular cytogenetics.

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Thomas Cremer
    • 1
  • Christoph Cremer
    • 2
  • Peter Lichter
    • 3
  1. 1.LMU BiozentrumMartinsriedGermany
  2. 2.Institute of Molecular Biology (IMB)MainzGermany
  3. 3.Division of Molecular GeneticsGerman Cancer Research Center (DKFZ)HeidelbergGermany