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Sequencing, assembly, annotation, and gene expression: novel insights into the hormonal control of carrot root development revealed by a high-throughput transcriptome

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Abstract

Previous studies have indicated that hormonal control is essential for plant root growth. The root of the carrot is an edible vegetable with a high nutritional value. However, molecular mechanisms underlying hormone-mediated root growth of carrot have not been illustrated. Therefore, the present study collected carrot root samples from four developmental stages, and performed transcriptome sequencing to understand the molecular functions of plant hormones in carrot root growth. A total of 160,227 transcripts were generated from our transcriptome, which were assembled into 32,716 unigenes with an average length of 1,453 bp. A total of 4,818 unigenes were found to be differentially expressed between the four developmental stages. In total, 87 hormone-related differentially expressed genes were identified, and the roles of the hormones are extensively discussed. Our results suggest that plant hormones may regulate carrot root growth in a phase-dependent manner, and these findings will provide valuable resources for future research on carrot root development.

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Acknowledgments

The research was supported by the New Century Excellent Talents in University (NCET-11-0670); Jiangsu Natural Science Foundation (BK20130027); China Postdoctoral Science Foundation (2014M551609), Priority Academic Program Development of Jiangsu Higher Education Institutions Project.

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The authors declare that there are no competing interests.

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Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Guang-Long Wang, Xiao-Ling Jia, Zhi-Sheng Xu, Feng Wang & Ai-Sheng Xiong

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  1. Guang-Long Wang
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  2. Xiao-Ling Jia
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  3. Zhi-Sheng Xu
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  4. Feng Wang
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  5. Ai-Sheng Xiong
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Correspondence to Ai-Sheng Xiong.

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Communicated by S.Hohmann.

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Wang, GL., Jia, XL., Xu, ZS. et al. Sequencing, assembly, annotation, and gene expression: novel insights into the hormonal control of carrot root development revealed by a high-throughput transcriptome. Mol Genet Genomics 290, 1379–1391 (2015). https://doi.org/10.1007/s00438-015-0999-5

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