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Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus

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Abstract

To determine if double-stranded RNA (dsRNA) viruses exist and are potential virulence factors in Trichomonas gallinae, virus purification via ultracentrifugation was attempted for 12 T. gallinae isolates recovered from wild birds. Following purification, virus-like particles were not observed by transmission electron microscopy, nor were dsRNA segments visualized in agarose gels after electrophoresis of extracted RNA from any of the 12 T. gallinae isolates. However, virus particles and dsRNA segments were detected from a previously determined virus-infected T. vaginalis isolate as a control using identical purification procedures. Subsequent reverse transcription-polymerase chain reaction analysis of the dsRNA of the virus in this isolate revealed a novel sequence of the RNA-dependent RNA polymerase gene of T. vaginalis viruses.

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Acknowledgements

This investigation was supported primarily by Cooperative Agreement No. 2001-96130032-CA, Veterinary Services, APHIS, USDA; Cooperative Agreement 01ERAG0013, United States Geological Survey, Biological Resources Division, USDI; and sponsorship of SCWDS by the fish and wildlife agencies of Alabama, Arkansas, Florida, Georgia, Kentucky, Kansas, Louisiana, Maryland, Mississippi, Missouri, North Carolina, Ohio, Puerto Rico, South Carolina, Tennessee, Virginia, and West Virginia. Support from the states to SCWDS was provided in part by the Federal Aid to Wildlife Restoration Act (50 Stat. 917). Additional support was given by Quail Unlimited’s Dove Sportsman Society. We thank Danny Watson of the Kentucky Department of Fish Wildlife Resources, Autumn Smith of the Caesar Kleberg Research Institute-Texas A&M University, Jeff Cann from the California Department of Fish and Game, and Bill Mannan from the School of Renewable Natural Resources, University of Arizona for collection of isolates. Additionally, we thank Mary Ard, University of Georgia, for assistance with the electronic magnification.

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Author notes

  1. Richard W. Gerhold

    Present address: Department of Poultry Science, The University of Georgia, Athens, GA, 30602, USA

  2. John F. Alderete

    Present address: School of Molecular Biosciences, Washington State University, Pullman, WA, 99164, USA

Authors and Affiliations

  1. Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, The University of Georgia, Athens, GA, 30602, USA

    Richard W. Gerhold & Andrew B. Allison

  2. Poultry Diagnostic and Research Center, College of Veterinary Medicine, The University of Georgia, Athens, GA, 30602, USA

    Holly Sellers & Erich Linnemann

  3. Department of Microbiology, The University of Texas Health Science Center, San Antonio, TX, 78229, USA

    T.-H. Chang

  4. Department of Poultry Science, The University of Georgia, Athens, GA, 30602-6772, USA

    Richard W. Gerhold

Authors
  1. Richard W. Gerhold
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  2. Andrew B. Allison
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  3. Holly Sellers
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  4. Erich Linnemann
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  5. T.-H. Chang
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  6. John F. Alderete
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Correspondence to Richard W. Gerhold.

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Gerhold, R.W., Allison, A.B., Sellers, H. et al. Examination for double-stranded RNA viruses in Trichomonas gallinae and identification of a novel sequence of a Trichomonas vaginalis virus. Parasitol Res 105, 775–779 (2009). https://doi.org/10.1007/s00436-009-1454-5

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  • DOI: https://doi.org/10.1007/s00436-009-1454-5

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