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Viral metagenomics reveals significant viruses in the genital tract of apparently healthy dairy cows

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Abstract

The virome in genital tract secretion samples collected from 80 dairy cattle in Shanghai, China, was characterized. Viruses detected included members of the families Papillomaviridae, Polyomaviridae, Hepeviridae, Parvoviridae, Astroviridae, Picornaviridae, and Picobirnaviridae. A member of a new species within the genus Dyoxipapillomavirus and six circular Rep-encoding single-stranded DNA (ssDNA) (CRESS-DNA) viral genomes were fully sequenced and phylogenetically analyzed. The prevalence of bovine polyomaviruses 1 and 2 was measured by PCR to be 10% (8/80) and 6.25% (5/80), respectively. PCR screening also indicated that the novel papillomavirus ujs-21015 and bovine herpesvirus 6 were present in three and two out of the 80 samples, respectively.

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Acknowledgments

This work was partly supported by National Key Research and Development Programs of China no. 2017YFC1200201, Jiangsu Provincial Key Research and Development Projects no. BE2017693, National Natural Science Foundation of China nos. 81741062 and 31572525, Taizhou Science and Technology Support Project no. TS201623, Key Research and Development Plan of Zhenjiang no. SH2016060, and Blood Systems Research Institute.

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Author notes

  1. Yu Ling and Xiaodan Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Microbiology, School of Medicine, Jiangsu University, 310 Xuefu Road, Zhenjiang, 202103, Jiangsu, China

    Yu Ling, Shixing Yang, Quan Shen, Xiaochun Wang & Wen Zhang

  2. Zhenjiang Center for Disease Prevention and Control, Zhenjiang, 212003, Jiangsu, China

    Xiaodan Zhang

  3. Institute of Animal Husbandry, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu, 611130, Sichuan, China

    Guilan Qi

  4. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Li Jingjiao, Li Cui & Xiuguo Hua

  5. Blood Systems Research Institute, San Francisco, CA, 94118, USA

    Xutao Deng & Eric Delwart

  6. Department of Laboratory Medicine, University of California, San Francisco, San Francisco, CA, 94118, USA

    Eric Delwart

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  1. Yu Ling
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Correspondence to Xiuguo Hua or Wen Zhang.

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Ling, Y., Zhang, X., Qi, G. et al. Viral metagenomics reveals significant viruses in the genital tract of apparently healthy dairy cows. Arch Virol 164, 1059–1067 (2019). https://doi.org/10.1007/s00705-019-04158-4

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