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Morphological phenotyping and genetic analyses of a new chemical-mutagenized population of tobacco (Nicotiana tabacum L.)

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Abstract

Main conclusion

A novel tobacco mutant library was constructed, screened, and characterized as a crucial genetic resource for functional genomics and applied research.

A comprehensive mutant library is a fundamental resource for investigating gene functions, especially after the completion of genome sequencing. A new tobacco mutant population induced by ethyl methane sulfonate mutagenesis was developed for functional genomics applications. We isolated 1607 mutant lines and 8610 mutant plants with altered morphological phenotypes from 5513 independent M2 families that consisted of 69,531 M2 plants. The 2196 mutations of abnormal phenotypes in the M2 putative mutants were classified into four groups with 17 major categories and 51 subcategories. More than 60% of the abnormal phenotypes observed fell within the five major categories including plant height, leaf shape, leaf surface, leaf color, and flowering time. The 465 M2 mutants exhibited multiple phenotypes, and 1054 of the 2196 mutations were pleiotropic. Verification of the phenotypes in advanced generations indicated that 70.63% of the M3 lines, 84.87% of the M4 lines, and 95.75% of the M5 lines could transmit original mutant phenotypes of the corresponding M2, M3, and M4 mutant plants. Along with the increased generation of mutants, the ratios of lines inheriting OMPs increased and lines with emerging novel mutant phenotypes decreased. Genetic analyses of 18 stably heritable mutants showed that two mutants were double recessive, five were monogenic recessive, eight presented monogenic dominant inheritance, and three presented semi-dominant inheritance. The pleiotropy pattern, saturability evaluation, research prospects of genome, and phenome of the mutant populations were also discussed. Simultaneously, this novel mutant library provided a fundamental resource for investigating gene functions in tobacco.

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Abbreviations

EMS:

Ethyl methane sulfonate

LD:

Lethal dose

NMP:

Novel mutant phenotype

OMP:

Original mutant phenotype

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Acknowledgements

We thank the members of The Agricultural Science and Technology Innovation Programs (ASTIP-TRIC01, ASTIP-TRIC02) who assisted with the mutant collection and characterization. This work was financially supported by the China Tobacco Genome Project [Grant Nos. 110201101009 (JY-03), 110201201004 (JY-04), and 110201301005 (JY-05)] and The Agricultural Science and Technology Innovation Program (ASTIP-TRIC02).

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  1. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, No. 11 Keyuanjingsi Road, Laoshan District, Qingdao, 266101, China

    Dawei Wang, Shaomei Wang, Jiangtao Chao, Xinru Wu, Yuhe Sun, Fengxia Li, Jing Lv, Xiaoming Gao, Guanshan Liu & Yuanying Wang

  2. Key Laboratory for Tobacco Gene Resources, State Tobacco Monopoly Administration, Qingdao, 266101, China

    Dawei Wang, Jiangtao Chao, Xinru Wu, Yuhe Sun, Fengxia Li, Jing Lv, Xiaoming Gao, Guanshan Liu & Yuanying Wang

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  1. Dawei Wang
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Wang, D., Wang, S., Chao, J. et al. Morphological phenotyping and genetic analyses of a new chemical-mutagenized population of tobacco (Nicotiana tabacum L.). Planta 246, 149–163 (2017). https://doi.org/10.1007/s00425-017-2690-z

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