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Identification of a novel homolog for a calmodulin-binding protein that is upregulated in alloplasmic wheat showing pistillody

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Abstract

Intracellular signaling pathways between the mitochondria and the nucleus are important in both normal and abnormal development in plants. The homeotic transformation of stamens into pistil-like structures (a phenomenon termed pistillody) in cytoplasmic substitution (alloplasmic) lines of bread wheat (Triticum aestivum) has been suggested to be induced by mitochondrial retrograde signaling, one of the forms of intracellular communication. We showed previously that the mitochondrial gene orf260 could alter the expression of nuclear class B MADS-box genes to induce pistillody. To elucidate the interactions between orf260 and nuclear homeotic genes, we performed a microarray analysis to compare gene expression patterns in the young spikes of a pistillody line and a normal line. We identified five genes that showed higher expression levels in the pistillody line. Quantitative expression analysis using real-time PCR indicated that among these five genes, Wheat Calmodulin-Binding Protein 1 (WCBP1) was significantly upregulated in young spikes of the pistillody line. The amino acid sequence of WCBP1 was predicted from the full-length cDNA sequence and found to encode a novel plant calmodulin-binding protein. RT-PCR analysis indicated that WCBP1 was preferentially expressed in young spikes at an early stage and decreased during spike maturation, indicating that it was associated with spikelet/floret development. Furthermore, in situ hybridization analysis suggested that WCBP1 was highly expressed in the pistil-like stamens at early to late developmental stages. These results indicate that WCBP1 plays a role in formation and development of pistil-like stamens induced by mitochondrial retrograde signaling.

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Abbreviations

CaM:

Calmodulin

CMS:

Cytoplasmic male sterility

Ctg:

Contig

CS:

Chinese Spring

MRS:

Mitochondrial retrograde signaling

N26:

Norin 26

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Acknowledgments

We are grateful to the National Bioresource Project—Wheat (NBRP–KOMUGI) for providing wheat materials. This work was supported in part by a Grant-in-Aid for Scientific Research (B) (No. 21380009) and a Grant-in-Aid for Scientific Research on Innovative Area (No. 24113517) from the Ministry of Education, Science and Culture of Japan (to K. M.).

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Authors and Affiliations

  1. Department of Bioscience, Fukui Prefectural University, 4-1-1 Matsuoka-kenjojima, Eiheiji-cho, Yoshida-gun, Fukui, 910-1195, Japan

    Mika Yamamoto, Naoki Shitsukawa, Maki Yamada, Keisuke Kato & Koji Murai

  2. Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501, Japan

    Shigeo Takumi

  3. Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Yokohama, 244-0813, Japan

    Kanako Kawaura & Yasunari Ogihara

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  1. Mika Yamamoto
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  2. Naoki Shitsukawa
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  3. Maki Yamada
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  4. Keisuke Kato
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  5. Shigeo Takumi
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  6. Kanako Kawaura
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  7. Yasunari Ogihara
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  8. Koji Murai
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Corresponding author

Correspondence to Koji Murai.

Additional information

The nucleotide sequence for WCBP1 cDNA reported in this paper has been submitted to the DDBJ/EMBL/GenBank database under the accession number AB716956. The wheat oligomicroarray with 37,826 probes is registered as GPL9805 in GEO at NCBI. A complete set of microarray data from this study was deposited to the GEO repository under the accession number GSM920888-920891.

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Yamamoto, M., Shitsukawa, N., Yamada, M. et al. Identification of a novel homolog for a calmodulin-binding protein that is upregulated in alloplasmic wheat showing pistillody. Planta 237, 1001–1013 (2013). https://doi.org/10.1007/s00425-012-1812-x

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