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Formation of hyaluronic acid–ellagic acid microfiber hybrid hydrogels and their applications

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Abstract

Microfiber assemblies prepared from ellagic acid (EA) were functionalized with histidine (His) and dispersed in hyaluronic acid (HA) hydrogel microstructures. Swelling studies indicated that the hybrids had a relatively lower water uptake compared to HA and was pH dependent. The percentage swelling ratio for EA–His–HA hybrids was 48 % when 0.04 mg/mL of HA was incorporated and increased to 70 % when 1.2 mg/mL HA was integrated. Release studies using the dye crystal violet (CV) as a model drug showed that the rates were concentration-dependent. Further the hybrids were found to be thermally stable compared to HA. Cellular toxicity assays performed with normal rat kidney (NRK) cells indicated biocompatibility and adherence of the hybrids to the cells. Thus, we have developed a new family of hybrid hydrogels which readily formed on the EA–His functionalized microfibers and may have potential applications in drug delivery or tissue regeneration applications.

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Acknowledgments

This work was conducted, in part, using equipment from the Queens College Facilities for Imaging, Cell and Molecular Biology. The authors thank Dr. Areti Tsiola for the use of the NanoDrop Spectrometer, as well as Dr. Patrick Brock and Dr. Barbara Balestra from the Geology Department at Queens College for the use of the scanning electron microscope. These studies were funded, in part, by the Fordham Dean’s Office (S.B., N.N., and S.F.), Fordham University Faculty research grant (I.B.), and grants from the PSC-CUNY Research Award Program (K.F.). S.B. also thanks the Campion Institute/Office of Prestigious Fellowships at Fordham University for their support.

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Authors and Affiliations

  1. Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, NY, 10458, USA

    Stacey N. Barnaby, Nako Nakatsuka, Stephen H. Frayne & Ipsita A. Banerjee

  2. Department of Biology, The City University of New York and The Graduate Center, Queens College, Flushing, NY, 11367, USA

    Karl R. Fath

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  1. Stacey N. Barnaby
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  2. Nako Nakatsuka
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Correspondence to Ipsita A. Banerjee.

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Barnaby, S.N., Nakatsuka, N., Frayne, S.H. et al. Formation of hyaluronic acid–ellagic acid microfiber hybrid hydrogels and their applications. Colloid Polym Sci 291, 515–525 (2013). https://doi.org/10.1007/s00396-012-2739-2

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