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Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria

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Abstract

A Gram-positive, moderately halophilic, endospore-forming bacterium, designated MerVT, was isolated from a sediment sample of a saline lake located in Ain Salah, south of Algeria. The cells were rod shaped and motile. Isolate MerVT grew at salinity interval of 0.5–25% NaCl (optimum, 5–10%), pH 6.0–12.0 (optimum, 8.0), and temperature between 10 and 40 °C (optimum, 30 °C).The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, a phospholipid, and two lipids, and MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C15:0 and anteiso C17:0. The DNA G+C content was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MerVT was most closely related to Virgibacillus halodenitrificans (gene sequence similarity of 97.0%). On the basis of phenotypic, chemotaxonomic properties, and phylogenetic analyses, strain MerVT (=DSM = 28944T) should be placed in the genus Virgibacillus as a novel species, for which the name Virgibacillus ainsalahensis is proposed.

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Authors and Affiliations

  1. Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria

    Meriam Amziane, Amel Darenfed-Bouanane, Ahmed Abderrahmani, Okba Selama, Lydia Jouadi & Farida Nateche

  2. Aix Marseille Université, IRD, Université de Toulon, CNRS, Mediterranean Institute of Oceanography (MIO), UM 110, Case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France

    Jean-Luc Cayol & Marie-Laure Fardeau

Authors
  1. Meriam Amziane
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  2. Amel Darenfed-Bouanane
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  3. Ahmed Abderrahmani
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  4. Okba Selama
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  5. Lydia Jouadi
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  6. Jean-Luc Cayol
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  8. Marie-Laure Fardeau
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Correspondence to Marie-Laure Fardeau.

Additional information

The GenBank/EMBL/DDBJaccesDDBJ accession number for the 16S rRNA genes sequence of strainT is KF146340.

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Amziane, M., Darenfed-Bouanane, A., Abderrahmani, A. et al. Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria. Curr Microbiol 74, 219–223 (2017). https://doi.org/10.1007/s00284-016-1171-0

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  • DOI: https://doi.org/10.1007/s00284-016-1171-0

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