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Virgibacillus litoralis sp. nov., a moderately halophilic bacterium isolated from saline soil

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Abstract

A Gram-positive, moderately halophilic, endospore-forming, catalase- and oxidase-positive, motile, rod-shaped, aerobic bacterium, designated strain JSM 089168T, was isolated from saline soil collected from Naozhou Island, Leizhou Bay, South China Sea. The organism was able to grow with 2–25% (w/v) total salts (optimum, 5–10%), at pH 6.0–10.0 (optimum, pH 8.0) and 10–45°C (optimum, 30°C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The strain contained MK-7 as the predominant menaquinone, and diphosphatidylglycerol and phosphatidylglycerol as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C15:0 and anteiso-C17:0, and the DNA G + C content was 40.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 089168T should be assigned to the genus Virgibacillus, being related most closely to the type strains of Virgibacillus carmonensis (sequence similarity 97.6%), Virgibacillus necropolis (97.3%) and Virgibacillus halodenitrificans (97.1%). Levels of DNA–DNA relatedness between strain JSM 089168T and the type strains of V. carmonensis, V. necropolis and V. halodenitrificans were 20.4, 14.3 and 12.0%, respectively. The combination of phylogenetic analysis, DNA–DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 089168T represents a novel species of the genus Virgibacillus, for which the name Virgibacillus litoralis sp. nov. is proposed. The type strain is JSM 089168T (=DSM 21085T =KCTC 13228T).

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (NSFC; 30860002, 30860013), Jishou University (jsdxkyzz200811), the Ministry of Science and Technology, PR China (2006BAE01A01-9, 863 Program 2007AA021306), The Key Projects of International Cooperation (2007DFB31620) and Yunnan University (2008YB005). We are grateful to Mr Huai-Dong Xiao and Mr Ke Huang for their excellent technical assistance.

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Authors and Affiliations

  1. College of Biology and Environmental Sciences, Department of Financial Management, Jishou University, Jishou, 416000, People’s Republic of China

    Yi-Guang Chen, Zhu-Xiang Liu, De-Jiao Peng & Yan-Qi Liu

  2. Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People’s Republic of China

    Yong-Xia Wang, Shu-Kun Tang, Wen-Jun Li & Xiao-Long Cui

  3. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, People’s Republic of China

    Yu-Qin Zhang

  4. Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Wen-Jun Li

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  1. Yi-Guang Chen
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Correspondence to Yan-Qi Liu.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 089168T is FJ425909.

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Chen, YG., Liu, ZX., Peng, DJ. et al. Virgibacillus litoralis sp. nov., a moderately halophilic bacterium isolated from saline soil. Antonie van Leeuwenhoek 96, 323–329 (2009). https://doi.org/10.1007/s10482-009-9349-0

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