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Halobacillus salicampi sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment

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Abstract

A Gram-positive, moderately halophilic bacterium, designated strain TGS-15T, was isolated from the sediment of a solar saltern pond located in Shinan, Korea. Strain TGS-15T was found to be a strictly aerobic, non-motile rod which can grow at pH 6.0–10.0 (optimum, pH 9.0), at 20–35 °C (optimum, 28 °C) and at salinities of 1–20 % (w/v) NaCl (optimum, 9 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TGS-15T belongs to the genus Halobacillus, with sequence similarity of 98.5–96.0 % to known type strains, showing high sequence similarity to Halobacillus locisalis MSS-155T (98.5 %), Halobacillus faecis IGA7-4T (98.2 %) and Halobacillus alkaliphilus FP5T (98.0 %), and less than 98.0 % sequence similarity to other currently recognised type strains of the genus. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The cell wall peptidoglycan was found to be based on L-Orn-D-Asp, the predominant isoprenoid quinone was identified as menaquinone-7 (MK-7) and the major fatty acids were identified as anteiso-C15:0, iso-C15:0, anteiso-C17:0 and C16:1 ω7c alcohol. The DNA G+C content of this novel isolate was determined to be 45.3 mol %. Levels of DNA:DNA relatedness between strain TGS-15T and the type strains of 13 other species of the genus ranged from 52 to 9 %. On the basis of the polyphasic analysis conducted in this study, strain TGS-15T is concluded to represent a novel species of the genus Halobacillus, for which the name Halobacillus salicampi sp. nov. is proposed. The type strain is TGS-15T (=KACC 18264T = NBRC 110640T).

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References

  • Amoozegar MA, Malekzadeh F, Malik KA, Schumann P, Spröer C (2003) Halobacillus karajensis sp. nov., a novel moderate halophile. Int J Syst Evol Microbiol 53:1059–1063

    Article  CAS  PubMed  Google Scholar 

  • An SY, Kanoh K, Kasai H, Yokota Y (2007) Halobacillus faecis sp. nov., a spore-forming bacterium isolated from a mangrove area on Ishigaki Island, Japan. Int J Syst Evol Microbiol 57:2476–2479

    Article  CAS  PubMed  Google Scholar 

  • Chen YG, Liu ZX, Zhang YQ, Zhang YX, Tang SK, Borrathybay E, Li WJ, Cui XL (2009) Halobacillus naozhouensis sp. nov., a moderately halophilic bacterium isolated from a sea anemone. Anto van Leeuwenh 96:99–107

    Article  Google Scholar 

  • Collins MD, Jones D (1981) Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354

    CAS  PubMed  PubMed Central  Google Scholar 

  • Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press, London

    Google Scholar 

  • DeLong EF (1992) Archaea in coastal marine environments. Proc Natl Acad Sci USA 89:5685–5689

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229

    Article  Google Scholar 

  • Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limit on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  • Hua NP, Kanekiyo A, Fujikura K, Yasuda H, Nagamura T (2007) Halobacillus profundi sp. nov. and Halobacillus kuroshimensis sp. nov., moderately halophilic bacteria solated from a deep-sea methanecold seep. Int J Syst Evol Microbiol 57:1243–1249

    Article  CAS  PubMed  Google Scholar 

  • Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, Park SC, Jeon YS, Lee JH, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721

    Article  CAS  PubMed  Google Scholar 

  • Kim SJ, Lee JC, Han SI, Whang KS (2015) Halobacillus sediminis sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment. Int J Syst Evol Microbiol 65:4434–4440

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions 301 through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Lányi B (1987) Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67

    Article  Google Scholar 

  • Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  • Leifson E (1963) Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184

    CAS  PubMed  PubMed Central  Google Scholar 

  • Liu WY, Zeng J, Wang L, Dou YT, Yang SS (2005) Halobacillus dabanensis sp. nov., and Halobacillus aidingensis sp. nov., isolated from salt lakes in Xinjiang. China. Int J Syst Evol Microbiol 55:1991–1996

    Article  CAS  PubMed  Google Scholar 

  • Logan NA, Berge O, Bishop AH, Busse HJ, De Vos P, Fritze D, Heyndrickx M, Kämpfer P, Rabinovitch L, Salkinoja-Salonen MS, Seldin L, Ventosa A (2009) Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121

    Article  CAS  PubMed  Google Scholar 

  • Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  • Murray RGE, Doetsch RN, Robinow F (1994) Determinative and cytological light microscopy. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 21–41

    Google Scholar 

  • Nunes I, Tiago I, Pires AL, da Costa MS, Veríssimo A (2006) Paucisalibacillus globulus gen. nov., sp. nov., a Gram-positive bacterium isolated from potting soil. Int J Syst Evol Microbiol 56:1841–1845

    Article  CAS  PubMed  Google Scholar 

  • Parte AC (2015) http://www.bacterio.net List of prokaryotic names with standing in nomenclature

  • Romano I, Finore I, Nicolaus G, Huertas FJ, Lama L, Nicolaus B, Poli A (2008) Halobacillus alkaliphilus sp. nov., a halophilic bacterium isolated from a salt lake in Fuente de Piedra, southern Spain. Int J Syst Evol Microbiol 58:886–890

    Article  PubMed  Google Scholar 

  • Saito H, Miura KI (1963) Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629

    Article  CAS  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Schleifer KH, Kandler O (1972) Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477

    CAS  PubMed  PubMed Central  Google Scholar 

  • Shida O, Takagi H, Kadowaki K, Nakamura LK, Komagata K (1997) Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47:289–298

    Article  CAS  PubMed  Google Scholar 

  • Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654

    Google Scholar 

  • Soto-Ramírez N, Sánchez-Porro C, Rosas-Padilla S, Almodóvar K, Jiménez G, Machado-Rodríguez M, Zapata M, Venrosa A, Montalvo-Rordíguez R (2008) Mangrovei sp. nov., a moderately halophilic bacterium isolated from the black mangrove Avicenniagerminans. Int J Syst Evol Microbiol 58:125–130

    Article  PubMed  Google Scholar 

  • Spring S, Ludwig W, Marquez MC, Ventosa A, Schleifer KH (1996) Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov., and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov. Int J Syst Bacteriol 46:492–496

    Article  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis. Version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tomaoka J, Komagata K (1984) Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128

    Article  Google Scholar 

  • Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Krichevsky MI, Moore LH, Moore WEC, Murray RGE, Stackebrandt E, Starr MP, Trüper HG (1987) International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approches to bacterial systematics. Int J Syst Bacteriol 37:463–464

    Article  Google Scholar 

  • Yabuuchi E, Yano I, Oyaizu H, Hashimoto Y, Ezaki T, Yamamoto H (1990) Proposals of Sphingomonas paucimobilis gen. nov. and comb. nov., Sphingomonas parapaucimobilis sp. nov., Sphingomonas yanoikuyae sp. nov., Sphingomonas adhaesiva sp. nov., Sphingomonas capsulata comb. nov., and two genospecies of the genus Sphingomonas. Microbiol Immunol 34:99–119

    Article  CAS  PubMed  Google Scholar 

  • Yabuuchi E, Kosako Y, Naka T, Suzuki S, Yano I (1999) Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown 1990) comb. nov., Sphingomonas natatoria (Sly 1985) comb. nov., Sphingomonas ursincola (Yurkov et al. 1997) comb. nov., and emendation of the genus Sphingomonas. Microbiol Immunol 43:339–349

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Weiss N, Lee KC, Lee IS, Kang KH, Park YH (2001) Jeotgalibacillus alimentarius gen. nov., sp. nov., a novel bacterium isolated from jeotgal with l-lysine in the cell wall, reclassification of Bacillus marinus Rüger 1983 as Marinibacillus marinus gen. nov., comb. nov. Int J Syst Evol Microbiol 51:2087–2093

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang KH, Park YH (2003) Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea. Int J Syst Evol Microbiol 53:687–693

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang KH, Oh TK, Park YH (2004) Halobacillus locisalis sp. nov., a halophilic bacterium isolated from a marine solar saltern of the Yellow Sea in Korea. Extremophiles 8:23–28

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang KH, Oh TK, Park YH (2005) Halobacillus yeomjeoni sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 55:2413–2417

    Article  CAS  PubMed  Google Scholar 

  • Yoon JH, Kang SJ, Jung YT, Oh TK (2007) Halobacillus campisalis sp. nov., containing meso-diaminopimelicacid in the cell-wall peptidoglycan, and emended description of the genus Halobacillus. Int J Syst Evol Microbiol 57:021–2025

    Google Scholar 

  • Yoon JH, Kang SJ, Oh TK (2008) Halobacillus seohaensis sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 58:622–627

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was supported by a grant from the Regional SubGenBank Support Program of the Rural Development Administration, Republic of Korea.

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    Authors and Affiliations

    1. Department of Microbial & Nano Materials, College of Science & Technology, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 302-318, Republic of Korea

      Su-Jin Kim, Jae-Chan Lee, Song-Ih Han & Kyung-Sook Whang

    2. Institute of Microbial Ecology and Resources, Mokwon University, 88 Doanbuk-ro, Seo-gu, Daejeon, 302-318, Republic of Korea

      Jae-Chan Lee & Kyung-Sook Whang

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    1. Su-Jin Kim
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    Correspondence to Kyung-Sook Whang.

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    Su-Jin Kim and Jae-Chan Lee have contributed equally to this work.

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    Kim, SJ., Lee, JC., Han, SI. et al. Halobacillus salicampi sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment. Antonie van Leeuwenhoek 109, 713–720 (2016). https://doi.org/10.1007/s10482-016-0672-y

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