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Two Novel Exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and Effect on Oxidative Stress

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Abstract

Two odorless, water-soluble exopolysaccharide (EPS) fractions, EPS-1 and EPS-2, were isolated from a newly isolated Bacillus amyloliquefaciens strain C-1 and purified by ion exchange and gel chromatography. The purified EPS-1 contained glucose/mannose/galactose/arabinose in a relative proportion of 15:4:2:1, and possessed a molecular weight of 79.6 kDa, while EPS-2 contained only glucose and mannose in a 3:1 ratio, with the molecular weights of 19.8 kDa. The antioxidant activity results showed that EPS-1 exhibited strong reducing power, superoxide radicals (O2−·), and hydroxyl free radicals (OH·) scavenging activities. For the H2O2-induced injury in HepG2 cells, EPS-1 significantly decreased the formation of reactive oxygen species, intracellular malondialdehyde levels, and restored intracellular superoxide dismutase activity. For EPS-2, there had no detectable antioxidant activities. And all these results collectively showed that as a natural antioxidant, only EPS-1 produced by C-1 had considerable potential to be used as medical compounds or functional additives.

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Acknowledgments

This work was financially supported by the Natural Science Foundation of China (31200031), the Fundamental Research Funds for the Central Universities (xjj2012125), Open Project Program of the State Key Laboratory of Food Science and Technology, Nanchang University (SKLF-KF-201201), and China Postdoctoral Science Foundation funded Project (2013M532055).

Conflict of interest

The authors declare that they have no competing interests to this paper.

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Authors and Affiliations

  1. Health Science Center, Xi’an Jiaotong University, P.O. 44, #76 Yanta West Road, Xi’an, 710061, Shaanxi, China

    Haixia Yang, Yue Yuan, Ruijuan Zhang & Bei Han

  2. Shaanxi Key Laboratory of Degradable Biomedical Materials, Department of Food Science and Engineering, College of Chemical Engineering, Northwest University, Xi’an, 710069, China

    Jianjun Deng & Daidi Fan

  3. Department of Neuroscience Training, University of Louisville, School of Medicine, Louisville, KY, 40202, USA

    Yan Zhang

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  1. Haixia Yang
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  2. Jianjun Deng
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  3. Yue Yuan
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  4. Daidi Fan
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  5. Yan Zhang
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  6. Ruijuan Zhang
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  7. Bei Han
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Correspondence to Bei Han.

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Yang, H., Deng, J., Yuan, Y. et al. Two Novel Exopolysaccharides from Bacillus amyloliquefaciens C-1: Antioxidation and Effect on Oxidative Stress. Curr Microbiol 70, 298–306 (2015). https://doi.org/10.1007/s00284-014-0717-2

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  • DOI: https://doi.org/10.1007/s00284-014-0717-2

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