Skip to main content
SpringerLink
Log in

Physicochemical characterization and antioxidant activity of a polysaccharide isolated from oolong tea

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

Oolong tea polysaccharide (OTPS) is a kind of water-soluble polysaccharide isolated from oolong tea. Its molecular weight, composition, and structure were analyzed by laser light scattering analysis, chemical methods, gas chromatography, and atomic force microscopy (AFM). Antioxidant potential was detected by hydroxyl radical scavenging, inhibition of lipid peroxidation, DPPH radical scavenging, and reducing power assays. Results showed that OTPS was a non-reductive acid heteropolysaccharide bound to protein. The amounts of uronic acid, protein and neutral sugar in OTPS were 40.65, 19.59, and 26.66%, respectively. The neutral sugar was composed of d-rhamnose, l-arabinose, d-galactose, and d-glucose with a molar ratio of 1.37:1.89:1.00:1.30. The molecular weight of OTPS was 1.28 × 106 Da and the second viral coefficient was 2.36 × 10−4 cm3 mol/g2. A globular image of OTPS was imaged by AFM. OTPS showed effective antioxidant activities on hydroxyl radical scavenging assay and inhibition assay of lipid peroxidation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Lin JK, Lin CL, Liang YC, Lin-Shiau SY, Juan IM (1998) J Agric Food Chem 46:3635–3642

    Article  CAS  Google Scholar 

  2. Tanida M, Tsuruoka N, Shen J, Kiso Y, Nagai K (2008) Metab Clin Exp 57:526–534

    CAS  Google Scholar 

  3. Han LK, Takaku T, Li J, Kimura Y, Okuda H (1999) Int J Obes Relat Metab Disord 23:98–105

    Article  CAS  Google Scholar 

  4. Chen HX, Zhang M, Xie BJ (2004) J Agric Food Chem 52:3333–3336

    Article  CAS  Google Scholar 

  5. Chen HX, Zhang M, Qu ZS, Xie BJ (2008) Food Chem 106:559–563

    Article  CAS  Google Scholar 

  6. Wang DF, Wang CH, Li J, Zhao GW (2001) J Agric Food Chem 49:507–510

    Article  CAS  Google Scholar 

  7. Ye H, Wang KQ, Zhou CH, Liu J, Zeng XX (2008) Food Chem 111:428–432

    Article  CAS  Google Scholar 

  8. Tao YZ, Zhang LN, Cheung PCK (2006) Carbohydr Res 341:2261–2269

    Article  CAS  Google Scholar 

  9. Qi HM, Zhang QB, Zhao TT, Chen R, Zhang H (2005) Int J Biol Macromol 37:195–199

    Article  CAS  Google Scholar 

  10. Zhang J, Wu J, Liang JY, Hu ZA, Wang YP, Zhang ST (2007) Carbohydr Polym 67:213–218

    Article  CAS  Google Scholar 

  11. Sun RG, Zhang J (2006) Acta Chim Sin 64:2467–2472

    CAS  Google Scholar 

  12. Ikeda S, Shishido Y (2005) J Agric Food Chem 53:786–791

    Article  CAS  Google Scholar 

  13. Chen HX, Zhang M, Xie BJ (2005) Food Chem 90:17–21

    Article  CAS  Google Scholar 

  14. Pushpamali WA, Nikapitiya C, Zoysa MD, Whang I, Kim SJ, Lee J (2008) Carbohydr Polym 73:274–279

    Article  CAS  Google Scholar 

  15. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  16. Bitter T, Muir HM (1962) Anal Biochem 4:330–334

    Article  CAS  Google Scholar 

  17. Bradford MM (1976) Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  18. Chaplin MF, Kennedy JF (1994) Carbohydrate analysis: a practical approach. Oxford University Press, London

    Google Scholar 

  19. Halliwell B, Gutteridge JMC, Aruoma OI (1987) Anal Chem 165:215–219

    CAS  Google Scholar 

  20. Liu XL, Dong MS, Chen XH, Jiang M, Lv X, Yan GJ (2007) Food Chem 105:548–554

    Article  CAS  Google Scholar 

  21. Anup S, Shereen RH, Shivanandappa T (2006) LWT Food Sci Technol 36:1059–1065

    Google Scholar 

  22. Oyaizu M (1986) Jpn J Nutr 44:307–315

    CAS  Google Scholar 

  23. Chen HX, Wang ZS, Lu XM, Xie BJ (2008) J Sci Food Agric 88:2523–2528

    Article  CAS  Google Scholar 

  24. Ioan CE, Aberle T, Burchard W (1999) Macromolecules 32:7444–7453

    Article  CAS  Google Scholar 

  25. Tao YZ, Zhang LN, Cheung PCK (2007) Carbohydr Polym 67:213–218

    Article  Google Scholar 

  26. Xu WT, Zhang FF, Luo YB, Ma LY, Kou XH, Huang KL (2009) Carbohydr Res 344:217–222

    Article  CAS  Google Scholar 

  27. Kuda T, Ikemori T (2009) Food Chem 112:575–581

    Article  CAS  Google Scholar 

  28. Giovanelli G, Buratti S (2008) Food Chem 112:903–908

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful for the financial support for this research from the National Natural Science Foundation of China (Grant No. 30600470).

Author information

Authors and Affiliations

  1. Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, People’s Republic of China

    Haixia Chen, Zhaoshuai Wang, Zhishuang Qu, Lingling Fu, Peng Dong & Xin Zhang

Authors
  1. Haixia Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhaoshuai Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhishuang Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lingling Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Peng Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Haixia Chen.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, H., Wang, Z., Qu, Z. et al. Physicochemical characterization and antioxidant activity of a polysaccharide isolated from oolong tea. Eur Food Res Technol 229, 629–635 (2009). https://doi.org/10.1007/s00217-009-1088-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-009-1088-y

Keywords

Search

Navigation