Abstract
Halophilic archaeal strain R28T was isolated from the brown alga Laminaria produced at Dalian, Liaoning Province, China. The cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative, and formed red-pigmented colonies. Strain R28T was able to grow at 25–50 °C (optimum 42 °C), in the presence of 3.1–5.1 M NaCl (optimum 3.9 M NaCl), with 0.005–1.0 M MgCl2 (optimum 0.01 M MgCl2) and at pH 6.0–9.5 (optimum pH 7.0–7.5). The minimal NaCl concentration to prevent cell lysis was 15 % (w/v). The major polar lipids of the strain were identified as phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, and two glycolipids chromatographically identical to those of Halovenus aranensis CGMCC 1.11001T. The 16S rRNA gene and rpoB′ gene of strain R28T were phylogenetically related to the corresponding genes of Hvn. aranensis CGMCC 1.11001T (91.9–97.2 and 82.9 % nucleotide identity, respectively). The DNA G+C content of strain R28T was determined to be 56.3 mol%. The phenotypic, chemotaxonomic, and phylogenetic properties suggest that strain R28T (=CGMCC 1.10592T = JCM 17269T) represents a novel species of the genus Halovenus, for which the name Halovenus rubra sp. nov. is proposed.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31370054), the Grant from China Ocean Mineral Resources Research and Development Association (COMRA) Special Foundation (DY125-15-R-03), the Qinglan Project of Jiangsu Province and a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene and rpoB ′ gene sequences of strain R28T are HM159605 and KF573409, respectively.
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Han, D., Zhang, WJ., Cui, HL. et al. Halovenus rubra sp. nov., Isolated from Salted Brown Alga Laminaria . Curr Microbiol 70, 91–95 (2015). https://doi.org/10.1007/s00284-014-0685-6
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DOI: https://doi.org/10.1007/s00284-014-0685-6