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Characterization of rhizosphere prokaryotic diversity in a horizontal subsurface flow constructed wetland using a PCR cloning-sequencing based approach

  • Environmental Biotechnology
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Abstract

Performance of biological wastewater treatment systems may be related to the composition and activity of microbial populations they contain. However, little information is known regarding microbial community inhabiting these ecosystems. The purpose of this study was to investigate archaeal and bacterial diversity, using cultivation-independent molecular techniques, in a constructed wetland receiving domestic wastewater. Two 16S rRNA gene libraries were constructed using total genomic DNA and amplified by PCR using primers specific for archaeal and bacterial domains. A high microbial diversity was detected. The Proteobacteria phylum is the most abundant and diversified phylogenetic group representing 31.3 % of the OTUs, followed by the Bacteroidetes (14.8 %), Planctomycetales (13.8 %), Actinobacteria (12 %), and Chloroflexi (8.2 %). Sequences affiliated with minor phylogenetic divisions such as the TM7, Nitrospira, OP10, and BRC1 are represented by <6 % of total OTUs. The Archaea domain was represented by the Thaumarchaeota phylum dominated by the Candidatus Nitrososphaera genus.

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Acknowledgments

The authors are very grateful to technical assistance of the Genoscope sequencing and bioinformatics teams. We also thank the staff of the wastewater treatment plant in Ksour Essef (Tunisia) and the Tunis International Center for Environmental Technologies.

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Authors and Affiliations

  1. Institut de Génomique/CEA//CNRS-UMR 8030, Genoscope, 2, rue Gaston Crémieux, 91057, Evry, France

    Moez Bouali, Eric Pelletier, Sébastien Chaussonnerie, Denis Le Paslier & Abdelghani Sghir

  2. Université d’Evry Val d’Essonne, 50 avenue François Mitterrand, 91025, Evry, France

    Abdelghani Sghir

  3. Laboratoire d’Analyses, Traitement et Valorisation des Polluants de l’Environnement et des Produits, Faculté de Pharmacie, rue Avicenne, 5000, Monastir, Tunisia

    Moez Bouali & Amina Bakhrouf

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  1. Moez Bouali
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  2. Eric Pelletier
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Correspondence to Moez Bouali.

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Bouali, M., Pelletier, E., Chaussonnerie, S. et al. Characterization of rhizosphere prokaryotic diversity in a horizontal subsurface flow constructed wetland using a PCR cloning-sequencing based approach. Appl Microbiol Biotechnol 97, 4221–4231 (2013). https://doi.org/10.1007/s00253-012-4239-1

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  • DOI: https://doi.org/10.1007/s00253-012-4239-1

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