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Chemically inhibited ATP synthesis promoted detachment of different-age biofilms from membrane surface

  • Environmental biotechnology
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Abstract

This study investigated the response of different-age biofilms developed on membrane surface to a chemical uncoupler 3, 3′, 4′, 5-tetrachlorosalicylanilide (TCS). Results showed that adenosine triphosphate (ATP) dissipation caused by TCS would promote different-age biofilms detachment, whereas chemically inhibited cellular ATP synthesis subsequently suppressed autoinducer-2 (AI-2) and extracellular polymeric substances (EPS) production. The extent of biofilm detachment was found to be closely related to AI-2-regulated EPS contents of bacteria. It was revealed that energy dissipation induced biofilm detachability was controlled by AI-2 regulated cellular communication via AI-2-mediated EPS secretion. This study would lead to a new cleaning strategy of biologically fouled membrane.

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Authors and Affiliations

  1. Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore

    Huijuan Xu, Kaiwen Teo, Huiling Neo & Yu Liu

  2. Advanced Environmental Biotechnology Centre, Nanyang Environment & Water Research Institute, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore

    Yu Liu

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  1. Huijuan Xu
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  2. Kaiwen Teo
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  3. Huiling Neo
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  4. Yu Liu
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Correspondence to Yu Liu.

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Xu, H., Teo, K., Neo, H. et al. Chemically inhibited ATP synthesis promoted detachment of different-age biofilms from membrane surface. Appl Microbiol Biotechnol 95, 1073–1082 (2012). https://doi.org/10.1007/s00253-011-3770-9

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  • DOI: https://doi.org/10.1007/s00253-011-3770-9

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