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Duplex real-time PCR for the determination of wasabi (Eutrema wasabi) contents in horseradish (Armoracia rusticana) products applying the ΔΔct-method

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Abstract

Many products claim to contain wasabi. Due to its high price, original wasabi (Eutrema wasabi) is often replaced by its cheaper close relative horseradish (Armoracia rusticana). To analyze such products, we developed a duplex real-time PCR method determining the DNA content of wasabi and horseradish, applying the ΔΔct-method. The developed horseradish-specific system turned out to be highly specific within the plant family Brassicaceae. We validated this duplex real-time PCR system and present results from common commercial products. The system proved to be reliable for uncovering fraudulent practices in the wasabi product group.

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Acknowledgments

We thank the Food Control Authority of the Canton Zürich for providing the resources for this work.

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  1. Food Control Authority of the Canton Zürich, Zurich, Switzerland

    René Köppel & Thomas B. Bucher

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  1. René Köppel
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  2. Thomas B. Bucher
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Correspondence to René Köppel.

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Köppel, R., Bucher, T.B. Duplex real-time PCR for the determination of wasabi (Eutrema wasabi) contents in horseradish (Armoracia rusticana) products applying the ΔΔct-method. Eur Food Res Technol 242, 1111–1115 (2016). https://doi.org/10.1007/s00217-015-2615-7

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  • DOI: https://doi.org/10.1007/s00217-015-2615-7

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