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Supercritical fluid extraction as a method for fat content determination and preparative technique for fatty acid analysis in mesh feed for pigs

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Abstract

Objective of this investigation was supercritical fluid extraction (SFE) with CO2, as analytical technique for total fat extraction from food and feed samples. Most significant advantages of this technique, compared with the others, are safety, cleanness, and shorter analysis time. Its main limitation includes difficulty of extracting polar lipids, due to the non-polar character of the CO2, which is used as a solvent. Influence of added absorbent and co-solvent on the SFE of mash pig feed was investigated in this paper. Total fat content was determined for ten commercially available mesh pig feeds. Optimization of absorbent weight and volume of added co-solvent showed that the maximum yield of extracted fat has been achieved at adsorbent mass of 1.8 g and ethanol volume of 0.54 ml. Yields of fat extracted with both Soxhlet method (AOCS Method Ba 3-38) and SFE were plotted one against the other. There were no significant (p > 0.05) differences between results of these two methods, which were confirmed by low value for standard deviation (SD). SFE appears to be reliable, faster, and less hazardous alternative for traditional extraction method. Fatty acid compositions from lipid extracts obtained by both methods were determined by gas chromatography—flame ionization detection (GC–FID). Results obtained by SFE were far more representative than results obtained by analyzing Soxhlet extracts, and in consistence with literature results. SFE method gave significantly higher (p < 0.001) contents of polyunsaturated fatty acids (PUFA) than Sohxlet method, especially of linoleic acid, C18:2 n-6.

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References

  1. Katan MB, Zock PL, Mensink RP (1994) Effects of fats and fatty acid on blood lipids in humans: an overview. Am J Clin Nutr 60(1):1017–1022

    Google Scholar 

  2. Bernardo-Gil MG, Cardoso Lopes LM (2004) Supercritical fluid extraction of Cucurbita ficifolia seed oil. Eur Food Res Technol 219:593–597

    Article  CAS  Google Scholar 

  3. Okanović Đ, Ilić N, Ivanov D, Palić D, Drobnjaković R, Vukčević Č, Ikonić P (2010) Influence of linseed enriched diet on omega-3 fatty acid content in pork. Krmiva 52(4):189–194

    Google Scholar 

  4. Johnson RB, Barnett HJ (2003) Determination of fat content in fish feed by supercritical fluid extraction and subsequent lipid classification of extract by thin layer chromatography-flame ionization detection. Aquaculture 216:263–282

    Article  CAS  Google Scholar 

  5. Heikes DL, Scott B, Gorzovalitis NA (2001) Quantifitation of voilatile oils in ground cumin by supercritical fluid extraction an gas chromatography with flame ionization detection. J AOAC Int 84(4):1130–1134

    CAS  Google Scholar 

  6. Devittori C, Gumy D, Kusy A, Colarow L, Bertoli C, Lambelet P (2000) Supercritical fluid extraction of oil from millet bran. JAOCS 77(6):573–579

    Article  CAS  Google Scholar 

  7. Fratianni A, Caboni MF, Irano M, Panfili G (2002) A critical comparison between traditional methods and supercritical carbon dioxide extraction for the determination of tocochromanols in cereals. Eur Food Res Technol 215:353–358

    Article  CAS  Google Scholar 

  8. Domagała J, Sady M, Grega T, Pustkowiak H, Florkiewicz A (2010) The influence of cheese type and fat extraction method on the content of conjugated linoleic acid. J Food Compost Anal 23:238–243

    Article  Google Scholar 

  9. Ixtaina VY, Vega A, Nolesco SM, Tomás MC, Gimeno M, Bárzana E, Tecante A (2010) Supercritical carbon dioxide extraction of oil from Mexican chia seed (Salvia hispanica L.): characterization and process optimization. J Supercrit Fluids 55:192–199

    Article  CAS  Google Scholar 

  10. Sihvonen M, Järvenpää E, Hietaniemi V, Huopalahti R (1999) Advances in supercritical carbon dioxide technologies. Trends Food Sci Tech 10:217–222

    Article  CAS  Google Scholar 

  11. Lau HLN, Choo YM, Ma AN, Chuah CH (2006) Characterization and supercritical carbon dioxide extraction of palm oil (Elaeis Guineensis). J Food Lipids 13:210–221

    Article  CAS  Google Scholar 

  12. Temelli F (2009) Perspectives on supercritical fluid processing of fats and oils. J Supercrit Fluids 47:583–590

    Article  CAS  Google Scholar 

  13. Artz WE, Kinyanjui T, Cheryan M (2005) Solubility optimization of oil components in supercritical carbon dioxide. J Food Lipids 12:91–102

    Article  CAS  Google Scholar 

  14. Sahena F, Zaidul ISM, Karim AA, Abbas KA, Norulaini NAN, Omar AKM (2009) Application of supercritical CO2 in lipid extraction—a review. J Food Eng 95:240–253

    Article  CAS  Google Scholar 

  15. Barthet VJ, Daun JK (2002) An evaluation of supercritical fluid extraction as an analytical tool to determine fat in canola, flax, solin, and mustard. JAOCS 79(3):245–251

    Article  CAS  Google Scholar 

  16. Aro H, Järvenpää EP, Könkö K, Sihvonen M, Hietaniemi V, Huopalahti R (2009) Isolation and purification of egg yolk phospholipids using liquid extraction and pilot-scale supercritical fluid techniques. Eur Food Res Technol 228:857–863

    Article  CAS  Google Scholar 

  17. AOAC (1990) 1990 AOAC 950.46 official methods of analysis, 15th edn. J AOAC Int, Arlington

    Google Scholar 

  18. AOCS (2001) Method Ba 3-38. In: Firestone D (ed) Official methods and recommended practices, 5th edn. AOCS Press, Champaign

  19. Organic application note (2003) LECO Corporation, St. Joseph. http://www.leco.com/resources/application_note_subs/pdf/organic/-183.pdf

  20. Shih F, Daigle K (2001) Supercritical fluid extraction of encapsulated oil products. JAOCS 78(10):1057–1059

    Article  CAS  Google Scholar 

  21. Tanaka Y, Sakaki I, Ohkubo T (2004) Extraction of phospholipids from salmon Roe with supercritical carbon dioxide and an entrainer. J Oleo Sci 53(9):417–424

    Article  CAS  Google Scholar 

  22. Taher H, Al-Zuhair S, AlMarzouqui A, Hashim I (2011) Extracted fat from lamb meat by supercritical CO2 as feedstock for biodiesel production. Biochem Eng J 55:23–31

    Article  CAS  Google Scholar 

  23. Berg H, Mågård M, Johansson G, Mathiasson L (1997) Development of a supercritical fluid extraction method for determination of lipid classes and total fat in meats and its comparison with conventional methods. J Chromatogr A 785(1–2):345–352

    Article  CAS  Google Scholar 

  24. Bellaloui N, Burns HA, Gillen MA, Abbas HK, Zablotowicz RM, Mengistu A, Paris RL (2010) Soybean seed protein, oil, fatty acids, and mineral composition as influenced by soybean-corn rotation. Agric Sci 1(3):102–109

    Google Scholar 

  25. Ashraf-Khorassani M, Ude M, Doane-Weideman T, Tomczak J, Taylor LT (2002) Comparison of gravimetry and hydrolysis/derivatization/gass chromatography-mass spectrometry for quantitative analysis of fat from standard reference infant formula powder using supercritical fluid extraction. J Agric Food Chem 50:1822–1826

    Article  CAS  Google Scholar 

  26. King JW, Eller FJ, Snyder JM, Johnson JH, McKeith FK, Stites CR (1996) Extraction of fat from ground beef for nutrient analysis using analytical supercritical fluid extraction. Agric Food Chem 44:2700–2704

    Article  CAS  Google Scholar 

  27. Snyder JM, King JW, Jackson MA (1996) Fat content for nutritional labeling by supercritical fluid extraction and an on-line lipase catalyzed reaction. J Chromatogr A 750:201–207

    Article  CAS  Google Scholar 

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Acknowledgments

This work is a part of Integrated and Interdisciplinary Research Project No. III 46012, funded by Serbian Ministry of Science and Technological Development.

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Authors and Affiliations

  1. Institute of Food Technology, University of Novi Sad, Bul. Cara Lazara 1, 21000, Novi Sad, Serbia

    Dušica Ivanov, Radmilo Čolović, Jovanka Lević & Slavica Sredanović

  2. Department of Material Engineering, Faculty of Technology, University of Novi Sad, Bul. Cara Lazara 1, 21000, Novi Sad, Serbia

    Oskar Bera

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  1. Dušica Ivanov
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  2. Radmilo Čolović
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  3. Oskar Bera
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  4. Jovanka Lević
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  5. Slavica Sredanović
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Correspondence to Dušica Ivanov.

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Ivanov, D., Čolović, R., Bera, O. et al. Supercritical fluid extraction as a method for fat content determination and preparative technique for fatty acid analysis in mesh feed for pigs. Eur Food Res Technol 233, 343–350 (2011). https://doi.org/10.1007/s00217-011-1521-x

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  • DOI: https://doi.org/10.1007/s00217-011-1521-x

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