Abstract
Arsenic trioxide has been successfully used as a therapeutic in the treatment of acute promyelocytic leukemia (APL). Detailed monitoring of the therapeutic arsenic and its metabolites in various accessible specimens of APL patients can contribute to improving treatment efficacy and minimizing arsenic-induced side effects. This article focuses on the determination of arsenic species in saliva samples from APL patients undergoing arsenic treatment. Saliva samples were collected from nine APL patients over three consecutive days. The patients received 10 mg arsenic trioxide each day via intravenous infusion. The saliva samples were analyzed using high-performance liquid chromatography coupled with inductively coupled plasma mass spectrometry. Monomethylarsonous acid and monomethylmonothioarsonic acid were identified along with arsenite, dimethylarsinic acid, monomethylarsonic acid, and arsenate. Arsenite was the predominant arsenic species, accounting for 71.8 % of total arsenic in the saliva. Following the arsenic infusion each day, the percentage of methylated arsenicals significantly decreased, possibly suggesting that the arsenic methylation process was saturated by the high doses immediately after the arsenic infusion. The temporal profiles of arsenic species in saliva following each arsenic infusion over 3 days have provided information on arsenic exposure, metabolism, and excretion. These results suggest that saliva can be used as an appropriate clinical biomarker for monitoring arsenic species in APL patients.
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Introduction
Acute promyelocytic leukemia (APL) is a distinctive subtype of acute myelocytic leukemia characterized by reciprocal translocations between chromosomes 15 and 17. Many clinical trials have demonstrated that both new and relapsed APL patients can achieve complete remission after arsenic trioxide treatment [1–7]. Generally, APL patients receive intravenous infusions of an aqueous solution of inorganic arsenic over the course of 2–5 h [1, 2, 4, 7]. In spite of the good efficacy of arsenic treatment, fatalities of APL patients and arsenic-induced side effects, e.g., skin and gastrointestinal reactions, liver and cardiac dysfunction, and neuropathy, have also been reported [2, 4, 7–9]. With the aim of alleviating side effects and maximizing treatment efficacy, extensive efforts have been made to elucidate the mechanisms of action, such as the effects of arsenic on apoptosis, differentiation, degradation of oncogenic fusion proteins, and signal transduction [10–14], as well as metabolism of arsenic in APL patients [15–17].
Inorganic arsenic can be readily transformed in many organisms through a biological process consisting of alternating reduction and oxidative methylation reactions, which consequently leads to the formation of various arsenic metabolites [15, 18–22]. Monomethylarsonic acid (MMAV) and dimethylarsinic acid (DMAV) have commonly been detected in human urine, saliva, and blood [15, 16, 20–26]. However, monomethylarsonous acid (MMAIII) and dimethylarsinous acid (DMAIII), which are proposed as important intermediates in the transformation process of inorganic arsenic, have been detected only in human urine [15, 27–29]. Recently, thiolated methylated arsenicals have also been identified as metabolites of inorganic arsenic in human urine [30, 31].
Various arsenic metabolites exhibit differing toxicities. For instance, trivalent methylated arsenicals are more toxic than their pentavalent counterparts [12, 32–35], and the toxicities of thiolated arsenicals are greater than those of pentavalent oxygen-containing analogs [30, 36–38]. Owing to the extreme variations in the toxicity of arsenic metabolites, it is necessary to identify and quantify arsenic species in APL patients undergoing arsenic treatment. The speciation information will contribute to improve our understanding of arsenic metabolism and to the design of timely clinical intervention and personalized therapy.
Although previous studies have determined arsenicals in the urine and blood of APL patients undergoing arsenic treatment [15–17], arsenic speciation in the saliva of APL patients has not been reported. Salivary glands have high blood flows, and arsenic species in the blood could be distributed to saliva. Collection of saliva samples is noninvasive and readily achievable. Thus, determination of arsenic species in saliva can complement biomonitoring of arsenic species in urine and blood, serving as a potential biomarker for assessment of recent exposure. The primary objective of this work is to identify and quantify arsenic species in saliva samples from APL patients undergoing arsenic treatment. Achieving this objective involves the development of arsenic speciation analysis and the application of this technique to determining temporal profiles of arsenic species in saliva.
Materials and methods
Reagents
MMAIII and DMAIII were synthesized in the iodide form of CH3AsI2 and (CH3)2AsI according to the literature [39], and were kept at 4 and −20 °C, respectively. Monomethylmonothioarsonic acid (MMMTAV) and dimethylmonothioarsinic acid (DMMTAV) were prepared according to a reported method [40], and were stored at −20 °C. Their stock solutions were freshly prepared in deionized water. Stock solutions of other standards (1,000 mg/L as arsenic) were prepared by dissolving appropriate amounts of AsIII, AsV, DMAV (all from Aldrich), and MMAV (Chem Service) in deionized water. Their working solutions were prepared daily by serial dilutions in deionized water.
Tetrabutylammonium hydroxide (Aldrich), malonic acid (Aldrich), and high-performance liquid chromatography (HPLC) grade methanol (Fisher) were used for preparing the mobile phase. The mobile phase was filtered through a 0.45-μm membrane filter.
A certified reference material (“Toxic Metals in Freeze-Dried Human Urine,” certified reference material no. 18 from the National Institute for Environmental Studies, Japan Environment Agency) was used for quality control purposes. This certified reference material was reconstituted by dissolving it in 20.0 mL deionized water according to the instructions provided by the supplier. The certified value for DMAV was 36 ± 9 μg/L.
APL patients and sample collection
Saliva samples were collected from nine patients who were undergoing arsenic treatment at Harbin Medical University Hospital. Informed consent was acquired from each patient prior to the study, which was conducted in compliance with the guidelines and regulations of the ethical review boards of the University of Alberta and Harbin Medical University Hospital (200816). Demographic information on the APL patients is given in Table 1. Two relapsed patients were returning for arsenic treatment, and the other patients were newly diagnosed with APL. Before the start of sample collection, two patients (one returning and one new) did not receive arsenic treatment, whereas the other patients had been treated with arsenic for differing durations, as shown in Table 1.
Ten milligrams of As2O3 (Yida Pharmaceutical, Harbin, China) dissolved in a 10-mL aqueous solution was added to 500 mL of 5 % glucose normal saline solution for intravenous infusion. The infusion usually takes 2–4 h. Arsenic was administered to patients 4, 5, and 9 over a period as long as 18 h because it caused them serious side effects. White blood cell counts and routine blood analysis were performed to monitor the health of APL patients. One saliva sample was collected from each patient immediately prior to daily infusion of arsenic. All patients then received intravenous infusions of the same dose of AsIII (10 mg As2O3), and saliva samples were collected consecutively in the subsequent 24 h before the next infusion. Sample collection was repeated for two or three 24-h cycles following different AsIII infusions. In total, 108 saliva samples were collected from nine APL patients. APL patients were required to rinse their mouths thoroughly with deionized water three times before saliva sampling; then they spat saliva samples directly into 15-mL polyethylene centrifuge tubes. Immediately after sample collection, saliva samples were divided into three 1-mL aliquots in 1.5-mL vials. The vials were sealed with Parafilm and transported on dry ice. The samples were stored at −20 °C until arsenic speciation analysis.
Pretreatment of saliva for arsenic speciation analysis
The method of pretreatment of saliva for arsenic speciation analysis has been described in the literature [23]. In brief, frozen saliva samples were thawed at room temperature and thoroughly vortex-mixed. A 0.5-mL aliquot of saliva samples was diluted three times with deionized water in a 15-mL centrifuge tube, and thoroughly vortex-mixed again. The diluted saliva samples were sonicated for 30 min, and then filtered through a 0.45-μm membrane filter prior to arsenic speciation analysis.
Identification and quantification of arsenic species by HPLC–inductively coupled plasma mass spectrometry
An Agilent 1100 series HPLC system consisting of a pump, degasser, autosampler, column temperature control, and reversed-phase C18 column (ODS-3, 150 mm × 4.6 mm, 3-μm particle size; Phenomenex, Torrance, CA, USA) was used for the separation of arsenicals. An octadecylsilane guard cartridge (4 mm × 3 mm) was mounted before the analytical column. The mobile phase consisted of 5 mM tetrabutylammonium, 5 % methanol, and 3 mM malonic acid (pH 5.65). The column was equilibrated with the mobile phase for at least 0.5 h at a flow rate of 0.8 mL/min before sample injection. A 50-μL aliquot of pretreated saliva samples was injected and separation was performed at a flow rate of 1.2 mL/min; the column temperature was maintained at 50 °C.
The effluent from HPLC was introduced directly into the nebulizer of a 7500ce inductively coupled plasma (ICP) mass spectrometry (MS) instrument (Agilent Technologies, Japan) using PEEK tubing. The collision cell of the ICP MS instrument was operated in helium mode. Helium (3.5 mL/min) was used in the octopole reaction cell to reduce isobaric and polyatomic interferences. The ICP operated at a radio-frequency power of 1,550 W, and the flow rate of argon carrier gas was 0.9–1.0 L/min. Arsenic was monitored at m/z 75. Chromatograms from HPLC separation were recorded by ICP-MS ChemStation (Agilent Technologies, Santa Clara, CA, USA).
Identification of MMMTAV by HPLC–electrospray ionization tandem mass spectrometry
For optimization of operating conditions, 1–5 μM MMMTAV in a solution of methanol and water (1:1, v/v) was infused into a triple-quadrupole mass spectrometer (5000 QTRAP, MDS SCIEX, Concord, ON, Canada) equipped with an electrospray ion source. The electrospray ionization (ESI) tandem mass spectrometry (MS/MS) instrument was operated in negative ionization mode. The characteristic multiple reaction monitoring transitions of MMMTAV were 155/107, 155/121, and 155/137. The optimal parameters were as follows: IonSpray voltage −4,500 V, interface temperature 200 °C, curtain gas flow rate 10 L/min, and declustering potential −75 V. The collision energy and the cell exit potential were −34 and −13 V for 155/107, -22 and −11 V for 155/121, and −22 and −11 V for 155/137.
The ESI-MS/MS instrument was coupled with an 1100 series HPLC system (Agilent, Santa Clara, CA, USA) equipped with a quaternary pump, degasser, column temperature control, and temperature-controlled autosampler. An anion-exchange column (PRP-X100, 50 mm × 4.6 mm, 10 μm; Hamilton, Reno, NV, USA) was used for separation, with the mobile phase comprising 50 % methanol and 5 mM ammonium formate (pH 6) at a flow rate of 1 mL/min. The temperature of the autosampler was kept at 4 °C, and the injection volume was 50 μL.
Results
Trivalent and thiolated monomethylated arsenicals, MMAIII and MMMTAV
Separation of eight arsenicals—AsIII, MMAIII, DMAV, MMAV, AsV, DMMTAV, DMAIII, and MMMTAV—was achieved using ion-pair chromatography, as shown in Fig. 1, chromatogram A. A typical chromatogram of saliva samples from APL patients undergoing arsenic treatment is shown in Fig. 1, chromatogram B. These results demonstrated that AsIII, MMAIII, DMAV, MMAV, AsV, and MMMTAV were detectable in the saliva of APL patients. The presence of MMAIII and MMMTAV in human saliva has not been reported in previous studies.
To confirm the presence of MMAIII and MMMTAV in the saliva of APL patients, we analyzed the saliva samples spiked with authentic standards of MMAIII or MMMTAV. Figure 2 shows a comparison of the chromatograms of the original sample and the same sample spiked with authentic standards. The spiked MMAIII or MMMTAV standard was co-eluted with suspected compounds, giving rise to a distinct increase in the intensity of peak corresponding to MMAIII or MMMTAV. The MMMTAV and MMAIII standards are not stable. MMMTAV can decompose easily into MMAIII, and thereafter MMAIII can be oxidized to MMAV. Even when stored at −80 °C after synthesis, MMMTAV still can produce some MMAIII and MMAV during the thawing process. Thus, spiking of MMMTAV also increased the intensities of the peaks of MMAIII and MMAV (Fig. 2, chromatograms B). Because MMAIII, MMAV, and MMMTAV were well resolved in the chromatogram, these spiking experiments could still demonstrate the existence of MMAIII and MMMTAV in the saliva of APL patients. The chromatographic fraction containing MMAIII was also collected and treated with hydrogen peroxide (H2O2) to render the oxidation of MMAIII to MMAV. As demonstrated in Fig. 3, detection of MMAV in an H2O2-pretreated MMAIII-containing chromatographic fraction provided further evidence that MMAIII was present in the saliva of APL patients.
ESI-MS/MS can provide information on the chemical structures of the compounds; therefore, an HPLC-ESI-MS/MS method was developed for the identification of MMMTAV. Unfortunately, the concentration of MMMTAV in the saliva of APL patients was below the detection limit of ESI-MS/MS. Nevertheless, the authenticity of the synthesized MMMTAV standard used was confirmed using the ESI-MS/MS method. As shown in Fig. 4, the peaks representing characteristic ion transitions of MMMTAV (155/107, 155/121, and 155/137) were superimposed, which demonstrated the authenticity of the MMMTAV standard synthesized in our laboratory.
The results for MMAIII and MMMTAV in the saliva of APL patients are summarized in Table 2. MMAIII and MMMTAV were detected in 49 % and 22 % of saliva samples from APL patients, respectively. The mean concentration of MMAIII in all detectable saliva samples was 1.4 ng/mL, and the mean concentration of MMMTAV was 3.6 ng/mL. MMAIII and MMMTAV accounted for 2.2 % and 4.0 % of total arsenic in the saliva of APL patients, respectively. Table 3 shows the concentrations of MMAIII and MMMTAV in the saliva of each of the APL patients. MMAIII was detected in saliva samples from each of the APL patients. However, MMMTAV was only found in the saliva of patients 3, 7, and 8. The mean concentrations of MMAIII and MMMTAV differed among the APL patients. The saliva of patient 8 contained the highest concentrations of MMAIII (1.9 ± 0.6 ng/mL) and MMMTAV (5.3 ± 2.9 ng/mL) among all patients.
AsIII, DMAV, MMAV, and AsV
The results for AsIII, DMAV, MMAV, and AsV in saliva samples from APL patients are also shown in Table 2. AsIII, DMAV, MMAV, and AsV were detectable in 98 % of saliva samples. The mean concentration was 39.1 ng/mL for AsIII, 9.2 ng/mL for AsV, 1.0 ng/mL for DMAV, and 2.0 ng/mL for MMAV. The summed concentration of all arsenicals in the saliva ranged from 0.1 to 210.1 ng/mL, with a mean concentration of 52.2 ng/mL. AsIII was the predominant species in the saliva of APL patients, and accounted for 71.8 % of arsenic in the saliva.
The arsenic concentration was low in two saliva samples collected from patients 1 and 4 before the first arsenic injection (Table 3). The saliva of patient 1 contained only 0.3 ng/mL AsIII, and the saliva of patient 4 contained 0.1 ng/mL DMAV and 0.1 ng/mL AsV. The rest of the saliva samples were collected after the first infusion of arsenic, and the concentrations of arsenic were much higher than in the samples collected before any infusion of arsenic. AsIII, DMAV, MMAV, and AsV were detected in all saliva samples collected after the first infusion of arsenic, and AsIII was consistently the predominant arsenic species in the saliva of all patients. Although the same dose of arsenic trioxide was administered to all APL patients, the mean concentrations of arsenicals differed among individual APL patients. Patients 2, 3, 7, and 8 had higher concentrations of arsenic in the saliva compared with the other patients.
Temporal profiles of common arsenic species
Figure 5 shows the temporal profiles of four common arsenic species (AsIII, DMAV, MMAV, and AsV) and their sum in the saliva of patients 4 and 6. Before the study, patient 4 did not receive an arsenic injection, but arsenic was administered to patient 6 (relapsed) for 11 days. Saliva samples were consecutively collected for 72 h from patient 4 (Fig. 5a) and for 52 h from patient 6 (Fig. 5b). Three infusions of arsenic were administered to patient 4 during the sample collection period, and two infusions were administered to patient 6. The interval between arsenic infusions was 24 h. As shown in Fig. 5a, the arsenic concentration was low in the saliva sample of patient 4 before arsenic infusion. Following the first arsenic infusion, the concentrations of arsenicals in the saliva increased immediately, and decreased thereafter. The profiles were similar following the second and third infusions. Nevertheless, the overall level of arsenic in the saliva increased with the progression of arsenic treatment, indicating that arsenic had accumulated in the body of patient 4. Compared with patient 4, the background arsenic concentration in the first saliva sample of patient 6 (Fig. 5b) was higher because arsenic trioxide had been administered to this patient 11 times over 11 days prior to the study. The arsenic concentration in the saliva of patient 6 showed a delayed increase in response to arsenic infusion, and similar patterns of changes in the two subsequent monitoring cycles.
Temporal profiles of percentages of methylated arsenicals in the saliva of patients 4 and 6 are presented in Fig. 6. Following arsenic infusion, the percentages of MMAV, DMAV, and their sum decreased in the saliva of both patients, suggesting that the methylation efficiency of AsIII was inhibited by accumulating AsIII. However, there was a difference in the temporal profiles of the percentages of salivary arsenicals between patients 4 and 6. The percentage of methylated arsenicals in the saliva of patient 4 declined continuously from 35 % to 6 %, as shown in Fig. 6a. For patient 6, the percentages of methylated arsenicals decreased immediately following arsenic infusion, and increased thereafter (Fig. 6b). A comparably similar profile between the two patients was repeated only after the second infusion.
Discussion
Following arsenic trioxide administration, a portion of AsIII can be transformed into methylated arsenicals in the body of APL patients through the processes of biological methylation [19, 41]. Salivary glands have high blood flow, and chemicals and their metabolites in blood can be distributed in saliva through passive diffusion, active transport, and ultrafiltration [42–45]. Our previous efforts showed that AsIII and its metabolites are present in saliva in populations who were chronically exposed to arsenic via drinking water or from chromated copper arsenate treated wood in playgrounds [23–25]. No attention has been paid to arsenic speciation in human saliva in clinical settings, in which APL patients were acutely exposed to high doses of AsIII via intravenous infusion.
Most arsenic in the saliva of APL patients was detected as AsIII. MMAV and DMAV, metabolites of AsIII commonly found in human urine [15, 16, 20–26], were also detected in saliva of APL patients. As important intermediates in the transformation processes of AsIII, MMAIII and DMAIII were found in human urine in previous studies [15, 27–29]. Our present study demonstrates the presence of MMAIII in the saliva of APL patients who received a high dose of AsIII, which provides additional evidence of biomethylation of AsIII in the human body. The cytotoxicity and genotoxicity of MMAIII were shown to be greater than those of inorganic arsenic and pentavalent methylated arsenicals [12, 32–35]
Thiolated methylated arsenicals have recently been identified as arsenic metabolites in human urine and nails [30, 31]. The present study also shows the presence of MMMTAV in the saliva of APL patients (patients 3, 7, and 8) undergoing arsenic treatment. Little is known about how these thiolated metabolites of arsenic are produced in the human body. Previous studies demonstrated that thiolated arsenicals may be produced in the gastrointestinal tract of rats by intestinal flora [46, 47]. However, in our study arsenic was administered to APL patients via intravenous infusion, and it was unlikely that MMMTAV in the saliva of APL patients was produced in the gastrointestinal tract. Thiolated arsenicals could probably be formed using H2S produced from enzymatically catalyzed reactions in mammalian cells [48, 49]. Thiolated methylated arsenicals were shown to be more toxic than their oxygen-containing analogs and exhibited toxicity comparable to that of trivalent arsenicals [30, 36–38].
Most arsenic infused is excreted from the bodies of APL patients through urine [15, 50]. The temporal profiles of salivary arsenicals depicted in our present study might well indicate the overall process of arsenic infusion, metabolism, and tributary excretion from the body. Arsenic accumulated in the saliva of patient 4 in the initial stage of treatment, but in the saliva of patient 6 the arsenic concentration reached a steady state after the adaptation to arsenic treatment. Likewise, a steady state was also reached in the urine within 5 days after repeated ingestion of 125–1,000 μg AsIII daily [50].
Many efforts have been made to understand whether high doses of ingested AsIII can affect methylation of arsenic in the living body. It was demonstrated that the formation of DMAV could be effectively inhibited by heavy ingestion of AsIII [51, 52]. Compared with the general population, APL patients who received high doses of AsIII intravenously had a lower fraction of DMAV in their urine [15], an observation that was supported by the saliva analysis in the present study. Methylated arsenicals accounted for 24 % of arsenic in the saliva of a general population chronically exposed to arsenic in drinking water [23]. In contrast, the percentage of methylated arsenicals in the saliva of APL patients was only 7.8 %. Additionally, the percentage of methylated arsenicals in the saliva of APL patients significantly decreased following each AsIII infusion, reflecting possible saturation of arsenic methylation by the high levels of AsIII in the body of APL patients.
References
Shen ZX, Chen GQ, Ni JH, Li XS, Xiong SM, Qiu QY, Zhu J, Tang W, Sun GL, Yang KQ, Chen Y, Zhou L, Fang ZW, Wang YT, Ma J, Zhang P, Zhang TD, Chen SJ, Chen Z, Wang ZY (1997) Use of arsenic trioxide (As2O3) in the treatment of acute promyelocytic leukemia (APL): II. Clinical efficacy and pharmacokinetics in relapsed patients. Blood 89(9):3354–3360
Niu C, Yan H, Yu T, Sun HP, Liu JX, Li XS, Wu W, Zhang FQ, Chen Y, Zhou L, Li JM, Zeng XY, Yang RR, Yuan MM, Ren MY, Gu FY, Cao Q, Gu BW, Su XY, Chen GQ, Xiong SM, Zhang TD, Waxman S, Wang ZY, Chen Z, Hu J, Shen ZX, Chen SJ (1999) Studies on treatment of acute promyelocytic leukemia with arsenic trioxide: remission induction, follow-up, and molecular monitoring in 11 newly diagnosed and 47 relapsed acute promyelocytic leukemia patients. Blood 94(10):3315–3324
Zhu J, Chen Z, Lallemand-Breitenbach V, de The H (2002) How acute promyelocytic leukaemia revived arsenic. Nat Rev Cancer 2(9):705–713
Mathews V, George B, Lakshmi KM, Viswabandya A, Bajel A, Balasubramanian P, Shaji RV, Srivastava VM, Srivastava A, Chandy M (2006) Single-agent arsenic trioxide in the treatment of newly diagnosed acute promyelocytic leukemia: durable remissions with minimal toxicity. Blood 107(7):2627–2632
Quezada G, Kopp L, Estey E, Wells RJ (2008) All-trans-retinoic acid and arsenic trioxide as initial therapy for acute promyelocytic leukemia. Pediatr Blood Cancer 51(1):133–135
Leu L, Mohassel L (2009) Arsenic trioxide as first-line treatment for acute promyelocytic leukemia. Am J Health Syst Pharm 66(21):1913–1918
Wang H, Hao L, Wang X, Li J, Wu Q, Bian S (2010) Retrospective study of arsenic trioxide for childhood acute promyelocytic leukemia in China: a single-center experience. Int J Hematol 91(5):820–825
Westervelt P, Brown RA, Adkins DR, Khoury H, Curtin P, Hurd D, Luger SM, Ma MK, Ley TJ, DiPersio JF (2001) Sudden death among patients with acute promyelocytic leukemia treated with arsenic trioxide. Blood 98(2):266–271
Cashin R, Burry L, Peckham K, Reynolds S, Seki JT (2008) Acute renal failure, gastrointestinal bleeding, and cardiac arrhythmia after administration of arsenic trioxide for acute promyelocytic leukemia. Am J Health Syst Pharm 65(10):941–946
Chen GQ, Shi XG, Tang W, Xiong SM, Zhu J, Cai X, Han ZG, Ni JH, Shi GY, Jia PM, Liu MM, He KL, Niu C, Ma J, Zhang P, Zhang TD, Paul P, Naoe T, Kitamura K, Miller W, Waxman S, Wang ZY, de The H, Chen SJ, Chen Z (1997) Use of arsenic trioxide (As2O3) in the treatment of acute promyelocytic leukemia (APL): I. As2O3 exerts dose-dependent dual effects on APL cells. Blood 89(9):3345–3353
Zhu Q, Zhang JW, Zhu HQ, Shen YL, Flexor M, Jia PM, Yu Y, Cai X, Waxman S, Lanotte M, Chen SJ, Chen Z, Tong JH (2002) Synergic effects of arsenic trioxide and cAMP during acute promyelocytic leukemia cell maturation subtends a novel signaling cross-talk. Blood 99(3):1014–1022
Chen GQ, Zhou L, Styblo M, Walton F, Jing Y, Weinberg R, Chen Z, Waxman S (2003) Methylated metabolites of arsenic trioxide are more potent than arsenic trioxide as apoptotic but not differentiation inducers in leukemia and lymphoma cells. Cancer Res 63(8):1853–1859
Li L, Wang J, Ye RD, Shi G, Jin H, Tang X, Yi J (2008) PML/RARα fusion protein mediates the unique sensitivity to arsenic cytotoxicity in acute promyelocytic leukemia cells: mechanisms involve the impairment of cAMP signaling and the aberrant regulation of NADPH oxidase. J Cell Physiol 217(2):486–493
Zhang XW, Yan XJ, Zhou ZR, Yang FF, Wu ZY, Sun HB, Liang WX, Song AX, Lallemand-Breitenbach V, Jeanne M, Zhang QY, Yang HY, Huang QH, Zhou GB, Tong JH, Zhang Y, Wu JH, Hu HY, de The H, Chen SJ, Chen Z (2010) Arsenic trioxide controls the fate of the PML-RARα oncoprotein by directly binding PML. Science 328(5975):240–243
Wang Z, Zhou J, Lu X, Gong Z, Le XC (2004) Arsenic speciation in urine from acute promyelocytic leukemia patients undergoing arsenic trioxide treatment. Chem Res Toxicol 17(1):95–103
Fukai Y, Hirata M, Ueno M, Ichikawa N, Kobayashi H, Saitoh H, Sakurai T, Kinoshita K, Kaise T, Ohta S (2006) Clinical pharmacokinetic study of arsenic trioxide in an acute promyelocytic leukemia (APL) patient: speciation of arsenic metabolites in serum and urine. Biol Pharm Bull 29(5):1022–1027
Yoshino Y, Yuan B, Miyashita S, Iriyama N, Horikoshi A, Shikino O, Toyoda H, Kaise T (2009) Speciation of arsenic trioxide metabolites in blood cells and plasma of a patient with acute promyelocytic leukemia. Anal Bioanal Chem 393(2):689–697
Thomas DJ, Waters SB, Styblo M (2004) Elucidating the pathway for arsenic methylation. Toxicol Appl Pharmacol 198(3):319–326
Bentley R, Chasteen TG (2002) Microbial methylation of metalloids: arsenic, antimony, and bismuth. Microbiol Mol Biol Rev 66(2):250–271
Cullen WR, Reimer KJ (1989) Arsenic speciation in the environment. Chem Rev 89:713–764
Le XC (2001) Arsenic speciation in the environment and humans. In: Frankenberger WT Jr (ed) Environmental chemistry of arsenic. Dekker, New York, pp 95–116
Sun G, Xu Y, Li X, Jin Y, Li B, Sun X (2007) Urinary arsenic metabolites in children and adults exposed to arsenic in drinking water in Inner Mongolia, China. Environ Health Perspect 115(4):648–652
Yuan C, Lu X, Oro N, Wang Z, Xia Y, Wade TJ, Mumford J, Le XC (2008) Arsenic speciation analysis in human saliva. Clin Chem 54(1):163–171
Lew K, Yuan CG, Acker JP, Le XC (2008) Salivary arsenic as a biomarker for arsenic exposure. Cell Biol Toxicol 24:367–371
Lew K, Acker JP, Gabos S, Le XC (2010) Biomonitoring of arsenic in urine and saliva of children playing on playgrounds constructed from chromated copper arsenate-treated wood. Environ Sci Technol 44(10):3986–3991
Hall M, Chen Y, Ahsan H, Slavkovich V, van Geen A, Parvez F, Graziano J (2006) Blood arsenic as a biomarker of arsenic exposure: results from a prospective study. Toxicology 225(2–3):225–233
Le XC, Lu X, Ma M, Cullen WR, Aposhian HV, Zheng B (2000) Speciation of key arsenic metabolic intermediates in human urine. Anal Chem 72(21):5172–5177
Le XC, Ma M, Cullen WR, Aposhian HV, Lu X, Zheng B (2000) Determination of monomethylarsonous acid, a key arsenic methylation intermediate, in human urine. Environ Health Perspect 108(11):1015–1018
Del Razo LM, Styblo M, Cullen WR, Thomas DJ (2001) Determination of trivalent methylated arsenicals in biological matrices. Toxicol Appl Pharmacol 174(3):282–293
Raml R, Rumpler A, Goessler W, Vahter M, Li L, Ochi T, Francesconi KA (2007) Thio-dimethylarsinate is a common metabolite in urine samples from arsenic-exposed women in Bangladesh. Toxicol Appl Pharmacol 222(3):374–380
Mandal BK, Suzuki KT, Anzai K, Yamaguchi K, Sei Y (2008) A SEC-HPLC-ICP MS hyphenated technique for identification of sulfur-containing arsenic metabolites in biological samples. J Chromatogr B Anal Technol Biomed Life Sci 874(1–2):64–76
Petrick JS, Ayala-Fierro F, Cullen WR, Carter DE, Vasken Aposhian H (2000) Monomethylarsonous acid (MMA(III)) is more toxic than arsenite in Chang human hepatocytes. Toxicol Appl Pharmacol 163(2):203–207
Mass MJ, Tennant A, Roop BC, Cullen WR, Styblo M, Thomas DJ, Kligerman AD (2001) Methylated trivalent arsenic species are genotoxic. Chem Res Toxicol 14(4):355–361
Styblo M, Del Razo LM, Vega L, Germolec DR, LeCluyse EL, Hamilton GA, Reed W, Wang C, Cullen WR, Thomas DJ (2000) Comparative toxicity of trivalent and pentavalent inorganic and methylated arsenicals in rat and human cells. Arch Toxicol 74(6):289–299
Charoensuk V, Gati WP, Weinfeld M, Le XC (2009) Differential cytotoxic effects of arsenic compounds in human acute promyelocytic leukemia cells. Toxicol Appl Pharmacol 239(1):64–70
Naranmandura H, Ogra Y, Iwata K, Lee J, Suzuki KT, Weinfeld M, Le XC (2009) Evidence for toxicity differences between inorganic arsenite and thioarsenicals in human bladder cancer cells. Toxicol Appl Pharmacol 238(2):133–140
Suzuki S, Arnold LL, Pennington KL, Chen B, Naranmandura H, Le XC, Cohen SM (2010) Dietary administration of sodium arsenite to rats: relations between dose and urinary concentrations of methylated and thio-metabolites and effects on the rat urinary bladder epithelium. Toxicol Appl Pharmacol 244(2):99–105
Naranmandura H, Bu N, Suzuki KT, Lou Y, Ogra Y (2010) Distribution and speciation of arsenic after intravenous administration of monomethylmonothioarsonic acid in rats. Chemosphere 81(2):206–213
Cullen WR, McBride BC, Manji H, Pickett AW, Reglinski J (1989) The metabolism of methylarsine oxide and sulfide. Appl Organometal Chem 3:71–78
Naranmandura H, Suzuki N, Iwata K, Hirano S, Suzuki KT (2007) Arsenic metabolism and thioarsenicals in hamsters and rats. Chem Res Toxicol 20(4):616–624
Challenger F (1945) Biological methylation. Chem Rev 36:315–361
Nigg HN, Stamper JH, Malory LL (1993) Quantification of human exposure to ethion using saliva. Chemosphere 26:897–906
Rafael G, Pascale B, Zul V, Paul H, Gideon K (1997) Is saliva suitable for therapeutic monitoring of anticonvulsants in children: an evaluation in the routine clinical setting. Ther Drug Monit 19:637–642
Denovan LA, Lu C, Hines CJ, Fenske RA (2000) Saliva biomonitoring of atrazine exposure among herbicide applicators. Int Arch Occup Environ Health 73(7):457–462
Barbosa F Jr, Tanus-Santos JE, Gerlach RF, Parsons PJ (2005) A critical review of biomarkers used for monitoring human exposure to lead: advantages, limitations, and future needs. Environ Health Perspect 113(12):1669–1674
Kuroda K, Yoshida K, Yoshimura M, Endo Y, Wanibuchi H, Fukushima S, Endo G (2004) Microbial metabolite of dimethylarsinic acid is highly toxic and genotoxic. Toxicol Appl Pharmacol 198(3):345–353
Yoshida K, Kuroda K, Zhou X, Inoue Y, Date Y, Wanibuchi H, Fukushima S, Endo G (2003) Urinary sulfur-containing metabolite produced by intestinal bacteria following oral administration of dimethylarsinic acid to rats. Chem Res Toxicol 16(9):1124–1129
Kamoun P (2004) Endogenous production of hydrogen sulfide in mammals. Amino Acids 26(3):243–254
Suzuki KT, Iwata K, Naranmandura H, Suzuki N (2007) Metabolic differences between two dimethylthioarsenicals in rats. Toxicol Appl Pharmacol 218(2):166–173
Buchet JP, Lauwerys R, Roels H (1981) Urinary excretion of inorganic arsenic and its metabolites after repeated ingestion of sodium metaarsenite by volunteers. Int Arch Occup Environ Health 48(2):111–118
Styblo M, Delnomdedieu M, Thomas DJ (1996) Mono- and dimethylation of arsenic in rat liver cytosol in vitro. Chem Biol Interact 99(1–3):147–164
Li J, Waters SB, Drobna Z, Devesa V, Styblo M, Thomas DJ (2005) Arsenic (+3 oxidation state) methyltransferase and the inorganic arsenic methylation phenotype. Toxicol Appl Pharmacol 204(2):164–169
Acknowledgments
We thank the patients and hospital staff for their participation in this study. This work was supported by the Canadian Institutes of Health Research, the Natural Sciences and Engineering Research Council of Canada, the Canada Research Chairs Program, Alberta Innovates, Alberta Health and Wellness, and the National Natural Science Foundation of China (21077033).
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B. Chen and F. Cao contributed equally to this paper.
Published in the topical collection Metallomics with guest editors Uwe Karst and Michael Sperling.
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Chen, B., Cao, F., Yuan, C. et al. Arsenic speciation in saliva of acute promyelocytic leukemia patients undergoing arsenic trioxide treatment. Anal Bioanal Chem 405, 1903–1911 (2013). https://doi.org/10.1007/s00216-012-6700-5
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DOI: https://doi.org/10.1007/s00216-012-6700-5