Abstract
The main aim of this study was to evaluate the toxicity of different poultry manure landfill leachates, using a well-known toxicity test system (MS3). The bioassay was made using a battery of toxicity tests including acute toxicity with crustacean (Daphnia magna), algae (Chlorella vulgaris) and the in vitro toxicity test with the fish cell line RTG-2. On D. magna was high mortality for zero time and almost 100 % and 70 %–80 % mortality for sawdust and straw poultry manure respectively. No effects on C. vulgaris, was observed after the leachate exposure. None of the parameters considered: protein, EROD activity, β-gal activity and neutral red, showed differences between control test and the leachate collected from exposure to poultry manure.
Similar content being viewed by others
References
Babin MM, Tarazona JV (2005) In vitro toxicity of selected pesticides on RTG-2 and RTL-W1 fish cell lines. Environ Pollut 135:267–274
Borenfreund E, Puerner JÁ (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol Lett 24:119–124
Delgado M, Martín JV, Miralles de Imperial R, León C, Garcia MC (2010) Phytotoxicity of uncomposted and composted poultry manure. Afr J Plant Sci 4(5):154–162
Delgado M, Rodriguez C, Martín JV, Miralles de Imperial R, Alonso F (2012) Environmental assay on the effect of poultry manure application on soil organisms in agro-ecosystem. Sci Total Environ 416:532–535
Dikinya O, Mufwanzala N (2010) Chicken manure-enhanced soil fertility and productivity: effects of application rates. Soil Sci Environ Manag 1(3):46–54
Fernández C, Cagigal E, Vega M, Tarazona JV (2005) Ecological risk assessment of contaminated soils through direct toxicity assessment. Ecotoxicol Environ Saf 62:174–184
Institute SAS (2001) SAS user’s guide: statistic. SAS inst, Cary, NC
ISO 6341 (1996) Water quality. Determination of the inhibition of the mobility of Daphnia magna straus (Cladocer, Crustacea). Acute toxicity test. 3rd edn
López-Masquera ME, Cabaleiro F, Sainz MS, López-Fabal A, Carral E (2008) Fertilizing value of broiler litter: effects of dryng and pelletizing. Bioresour Technol 99:5626–5633
Navas J, Babin M, Casado S, Fernandez C, Tarazona JV (2006) The Prestige oil spill: a laboratory study about the toxicity of the water-soluble faction of fuel oil. Marine Environ Res 62:s352–s355
OECD (organization for economic cooperation and development) (2004) OCDE guidelines for testing of chemicals. Daphnia sp acute toxicity test. OECD Guideline No 202, Paris, France 2004
Ramos C, de la Torre AI, Tarazona JV, Muñoz MJ (1996) Desarrollo de un ensayo de inhibición del crecimiento de Chlorella vulgaris utilizando un test en microplacas. Rev Toxicol 13:97–100
Slomczynska B, Slomczynski T (2004) Physico-chemical and toxicological characteristics of leachates from MSW landfills. Pol J Environ Stud 6:627–637
Vega M, Fernandez C, Blazquez T, Tarazona JV, Castaño A (1996) Biological and chemical tools in the toxicological risk assessment of Jarama river, Madrid Spain. Environ Pollut 93:135–139
Acknowledgments
We are grateful to INIA-FEDER for funding this study as part of the RTA2005-00120-CO2-01 and RTA2009-00074-00-00 projects. The authors wish to extend their sincere thanks to Ecotoxicology Laboratory for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Delgado, M., de Imperial, R.M., Alonso, F. et al. Ecotoxicity Bioassays on Leachates from Poultry Manure. Bull Environ Contam Toxicol 90, 401–404 (2013). https://doi.org/10.1007/s00128-012-0936-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-012-0936-9