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Clubroot resistance QTL are modulated by nitrogen input in Brassica napus

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Nitrogen levels can modulate the effectiveness of clubroot resistance in an isolate- and host-specific manner. While the same QTL were detected under high and low nitrogen, their effects were altered.

Abstract

Clubroot, caused by Plasmodiophora brassicae, is one of the most damaging diseases of oilseed rape and is known to be affected by nitrogen fertilization. However, the genetic factors involved in clubroot resistance have not been characterized under nitrogen-limiting conditions. This study aimed to assess the variability of clubroot resistance under different nitrogen levels and to characterize the impact of nitrogen supply on genetic resistance factors. Linkage analyses and a genome-wide association study were conducted to detect QTL for clubroot resistance and evaluate their sensitivity to nitrogen. The clubroot response of a set of 92 diverse oilseed rape accessions and 108 lines derived from a cross between ‘Darmor-bzh’ (resistant) and ‘Yudal’ (susceptible) was studied in the greenhouse under high- and low-nitrogen conditions, following inoculation with the P. brassicae isolates eH and K92-16. Resistance to each isolate was controlled by a major QTL and a few small-effects QTL. While the same QTL were detected under both high and low nitrogen, their effects were altered. Clubroot resistance to isolate eH, but not K92-16, was greater under a low-N supply versus a high-N supply. New sources of resistance were found among the oilseed rape accessions under both low and high-N conditions. The results are discussed relative to the literature and from a crop improvement perspective.

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Acknowledgements

We would like to acknowledge Cyril Falentin, Gwenaëlle Deniot and Gilles Lassalle for the production of the genetic maps. The authors are grateful to the technical teams for data collection, especially to Jocelyne Lemoine, Christine Lariagon and Kevin Gazengel, as well as to the technical team in charge of the greenhouses: Laurent Charlon and Patrick Rolland. We acknowledge the CRMPO (Centre Régional de Mesures Physiques de l’Ouest) for the technical assistance with N analyses. The authors thank the students of AGROCAMPUS OUEST who participated in the phenotyping and who carried out the firsts runs of the analysis. The authors would like to thank the BrACySol biological resource center (INRA Ploudaniel, France) for providing the seeds used in this study.

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    Authors and Affiliations

    1. IGEPP, AGROCAMPUS OUEST, INRA, Université de Rennes 1, 35650, Le Rheu, France

      A. Laperche, Y. Aigu, M. Jubault, M. Ollier, S. Guichard, P. Glory, A. Gravot & MJ. Manzanares-Dauleux

    2. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada

      SE. Strelkov

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    1. A. Laperche
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    2. Y. Aigu
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    Correspondence to MJ. Manzanares-Dauleux.

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    This study was funded by the French Association for the Promotion of Oilseed Crops Breeding (PROMOSOL). Yoann Aigu is the recipient of a 3-year PhD fellowship from the University of Rennes1.

    Additional information

    Communicated by Isobel AP Parkin.

    A. Laperche and Y. Aigu have contributed equally to the work.

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    Laperche, A., Aigu, Y., Jubault, M. et al. Clubroot resistance QTL are modulated by nitrogen input in Brassica napus . Theor Appl Genet 130, 669–684 (2017). https://doi.org/10.1007/s00122-016-2842-8

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