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Fine genetic mapping of RXopJ4, a bacterial spot disease resistance locus from Solanum pennellii LA716

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Abstract

The RXopJ4 resistance locus from the wild accession Solanum pennellii (Sp) LA716 confers resistance to bacterial spot disease of tomato (S. lycopersicum, Sl) caused by Xanthomonas perforans (Xp). RXopJ4 resistance depends on recognition of the pathogen type III effector protein XopJ4. We used a collection of Sp introgression lines (ILs) to narrow the RXopJ4 locus to a 4.2-Mb segment on the long arm of chromosome 6, encompassed by the ILs 6-2 and 6-2-2. We then adapted or developed a collection of 14 molecular markers to map on a segregating F2 population from a cross between the susceptible parent Sl FL8000 and the resistant parent RXopJ4 8000 OC7. In the F2 population, a 190-kb segment between the markers J350 and J352 cosegregated with resistance. This fine mapping will enable both the identification of candidate genes and the detection of resistant plants using cosegregating markers. The RXopJ4 resistance gene(s), in combination with other recently characterized genes and a quantitative trait locus (QTL) for bacterial spot disease resistance, will likely be an effective tool for the development of durable resistance in cultivated tomato.

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Acknowledgments

We thank R. Bart and S. Goritschnig for critical reading of the manuscript. This research was supported by the Two Blades Foundation and a National Science Foundation Graduate Research Fellowship (Awarded to M.S.).

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Authors and Affiliations

  1. Department of Plant and Microbial Biology, University of California, 111 Koshland Hall, Berkeley, CA, 94720, USA

    Molly Sharlach, Douglas Dahlbeck, Lily Liu, Joshua Chiu & Brian J. Staskawicz

  2. Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829, Köln, Germany

    José M. Jiménez-Gómez

  3. Department of Bioresource and Environmental Sciences, Kyoto Sangyo University, Kita-ku, Kyoto-shi, Kyoto, 603-8555, Japan

    Seisuke Kimura

  4. Department of Molecular Biology, Max Planck Institute for Developmental Biology, Spemannstrasse 37-39, 72076, Tübingen, Germany

    Daniel Koenig

  5. Department of Plant Biology, University of California, 1 Shields Ave., Davis, CA, 95616, USA

    Julin N. Maloof & Neelima Sinha

  6. Plant Pathology Department, University of Florida, 2515 Fifield Hall, P.O. Box 110680, Gainesville, FL, 32611, USA

    Gerald V. Minsavage, Jeffrey B. Jones & Robert E. Stall

Authors
  1. Molly Sharlach
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  2. Douglas Dahlbeck
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  3. Lily Liu
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  6. Seisuke Kimura
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  7. Daniel Koenig
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  8. Julin N. Maloof
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  9. Neelima Sinha
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  10. Gerald V. Minsavage
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  11. Jeffrey B. Jones
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  12. Robert E. Stall
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  13. Brian J. Staskawicz
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Corresponding authors

Correspondence to Molly Sharlach or Brian J. Staskawicz.

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Communicated by M. Havey.

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Sharlach, M., Dahlbeck, D., Liu, L. et al. Fine genetic mapping of RXopJ4, a bacterial spot disease resistance locus from Solanum pennellii LA716. Theor Appl Genet 126, 601–609 (2013). https://doi.org/10.1007/s00122-012-2004-6

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  • DOI: https://doi.org/10.1007/s00122-012-2004-6

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